XB-CLONE-3252063
Clone Name: IMAGE:7691140
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Gene:
cul5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121941 [+]
GGCGGGAAGACAAGTTGAGGGAACATGGCGACGTCTAATTTGTTAAAGAATAAAGGTTCCCTTCAATTTGAGGACAAATGGGACTTTATGAGACCTATAG
TTCTGAAACTTTTACGCCAGGAATCCGTCACAAAACAGCAATGGTTCGATTTGTTCTCAGATGTACATGCAGTTTGCCTATGGGATGACAAGGGCCCTGC
AAAAATTCATCAGGCTTTAAAAGAAGACATCCTTGATTTTATAAAGCAAGCTCAGGCTAGGGTACTAAGCCATCAAGATGACACTGCATTACTTAAAGCT
TACATTGTAGAGTGGCGCAAGTTCTTCACGCAATGTGACATCTTACCAAAGCCTTTTTGTCAACTAGAAATCACTTTAATGGGAAAGCAAGGCAGCAACA
AAAAATCCAACGTAGAAGATAGTATTGTACGAAAGCTGATGCTGGACACATGGAATGAATCTATTTTTTCTAATATAAAAAATCGACTTCAGGATAGCGC
AATGAAATTGGTCCATGCCGAGCGACTAGGAGAGGCTTTTGATTCTCAACTTGTAATAGGAGTGAGGGAGTCATATGTTAATTTATGTTCTAATCCGGAA
GACAAACTCCAGATCTACAGGGATAATTTCGAAAAAGCCTACTTGGATTCAACAGAGCGTTTCTACCGCACACAGGCCCCTTCCTATTTGCAGCAGAATG
GGGTACAGAACTATATGAAATATGCAGACGCTAAACTAAAGGAAGAAGAGAACAGAGCGCTCCGTTATCTAGAGACACGACGGGAATGTAACTCTGTGCA
GGCATTAATGGAATGTTGTGTAAATGCTCTTGTGACGTCCTTCAAAGAGACAATTTTAGCTGAATGTCAAGGCATGATCAAGCGCAATGAAACGGAAAAG
CTGCATTTAATGTTTTCCCTTATGGATAAAGTCCCTAATGGAATAGAACCCATGTTAAAAGATCTAGAAGAGCACATTGTAAGTGCTGGCTTGGCTGACA
TGGTGGCTGCTGCAGAAACCATTACTACTGATTCTGAAAAATATGTTGAACAATTACTGACCCTGTTTAATAGATTTAGTAAACTAGTAAAAGAAGCCTT
CCAGGACGATCCACGGTTTCTCACTGCACGAGATAAGGCATACAAAGCTGTTGTAAATGATGCAACAATATTTAAACTTGAATTGCCACTAAAGCAGAAA
GGGGTGGGTCTGAAAACACAGCCGGAGTCCAAGTGCCCAGAGTTGCTCGCTAACTATTGTGATATGTTGTTACGGAAAACGCCACTCAGTAAGAAACTGA
CTTCTGAGGAAATCGAGGCAAAGTTGAAGGAAGTGCTGTTGGTGCTAAAATATGTGCAGAACAAGGATGTGTTCATGAGGTACCACAAGGCTCACCTGAC
ACGCCGCCTCATTTTAGATATCTCCGCCGACAGTGAAATAGAAGAAAACATGGTGGAGTGGCTCAGGGAGGTGGGCATGCCAGCTGATTATGTAAATAAA
CTAGCCAGAATGTTCCAGGACATCAAAGTATCAGAAGACTTAAACCAGGCTTTCAAAGAAATGCATAAAAATAATAAATTAGCCCTGCCAGCTGACTCTG
TGAATATTAAAATTTTGAATGCCGGTGCTTGGTCACGGAGCTCGGAAAAGGTATTTGTGTCCCTTCCCACTGAGCTCGAGGACCTCATTCCTGAAGTAGA
GGAGTTTTACAAGAAAAACCACAGCGGCAGAAAGCTTCACTGGCACCATCTTATGTCCAATGGCATTATAACATTTAAGAATGAGGTTGGCCAGTACGAC
TTGGAAGTTACCACTTTCCAGCTGGCAGTTCTGTTTGCTTGGAACCAAAGGCCGAGAGAAAAAATCAGCTTTGAAAATCTTAAACTGGCAACAGAGTTAC
CTGATGCTGAGCTTAGGAGAACTCTGTGGTCTCTTGTAGCATTTCCAAAGCTGAAACGCCAAGTATTATCCTATGAACCGACGGTAAATTCCCCAAAAGA
CTTCACAGAGGGTACCCTCTTCTCTGTAAATCAGGAGTTCAGCCTAATAAAAAATGGAAAGGTTCAGAAACGGGGTAAGATCAACTTAATCGGCCGCTTG
CAGCTGACCACGGAAAGGATGCGAGAAGAAGAGAATGAAGGCATTGTGCAGCTAAGGATATTAAGGACACAGGAGGCTATTATTCAAATCATGAAGATGA
GGAAGAAAATTACTAACGCCCAGCTCCAGACTGAACTTGTAGAAATTTTAAAAAACATGTTTTTGCCACAGAAGAAAATGATAAAAGAGCAAATTGAATG
GCTCATAGAACACAAGTACATCAAGAGAGACGAATCCGACATTAACACCTTCATATACATGGCGTAATATGGACAGCCTATCGGTTGGTATGGAAAATTG
CCCACAAGTCCTTGGAGCCGGCCATCTCAATCATTCATGCTGAAAGCGAACTCCTGACTTTTGTAATGTGTAACTATTACAACCCCCTGCCTTATTTTAA
GGACAAAAAAAAAATATCATTGCCAATGTCATATTGACAGCATGACACTGTCTTCTTCTCCATGTTTCTTTGACCAGCATGCAGTTTTAATGCAAACTCC
TTTATGAAGAACTCGTTGTGAGCTTGTAAAGTAGATTTCATTTGGTTCGACCAGAGAAACCTAGTTGGTCCCCTCTCCCCCCCCAGCGGACGAGGACATC
ACATTTTCATGCACTGAGGAACTGCTCGAATTGTAGTTTTGTGTTTAAAGAAAAAACAAAAATACAAAAAAAAAAAAAAA
5' EST: CX916551 [+]gcgggaagacaagttgagggaacatggcgacgtctaatttgttaaagaataaaggttcccttcaatttgaggacaaatgggactttatgagacctatagt
tctgaaacttttacgccaggaatccgtcacaaaacagcaatggttcgatttgttctcagatgtacatgcagtttgcctatgggatgacaagggccctgca
aaaattcatcaggctttaaaagaagacatccttgattttataaagcaagctcaggctagggtactaagccatcaagatgacactgcattacttaaagctt
acattgtagagtggcgcaagttcttcacgcaatgtgacatcttaccaaagcctttttgtcaactagaaatcactttaatgggaaagcaaggcagcaacaa
aaaatccaacgtagaagatagtattgtacgaaagctgatgctggacacatggaatgaatctattttttctaatataaaaaatcgacttcaggatagcgca
atgaaattggtccatgccgagcgactaggagaggcttttgattctcaacttgtaataggagtgagggagtcatatgttaatttatgttctaatccggaag
acaaactccagatctacagggataatttcgaaaaagcctacttggattcaacagagcgtttctaccgcacacaggccccttcctatttgcagcagaa
3' EST: CX916550 [+]ctttaaacacaaaactacaattcgagcagttcctcagtgcatgaaaatgtgatgtcctcgtccgctggggggggagaggggaccaactaggtttctctgg
tcgaaccaaatgaaatctactttacaagctcacaacgagttcttcataaaggagtttgcattaaaactgcatgctggtcaaagaaacatggagaagaaga
cagtgtcatgctgtcaatatgacattggcaatgatattttttttttgtccttaaaataaggcagggggttgtaatagttacacattacaaaagtcaggag
ttcgctttcagcatgaatgattgagatggccggctccaaggacttgtgggcaattttccataccaaccgataggctgtccatattacgccatgtatatga
aggtgttaatgtcggattcgtctctcttgatgtacttgtgttctatgagccattcaatttgctcttttatcattttcttctgtggcaaaaacatgttttt
taaaatttctacaagttcagtctggagctgggcgttagtaattttcttcctcatcttcatgatttgaataatagcctcctgtgtccttaatatccttagc
tgcacaatgccttcattctcttcttctcgcatcctttccgtggtcagctgcaagcggccgattaagttgatcttaccccgtttctgaacctttccatttt
ttattaggctgaactcctgatttacag
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: