XB-CLONE-3251934
Clone Name: IMAGE:7691507
|
Gene:
stag3
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC161103 [+]
cgtttatgttgacgtcgtaaaagtcggtaaaagggaacgttgttgcggaacaattatacgcttgtgaagtcgggacgcatttacgtgttcaggatgcatc
actcacaccagaaatcctcctcagttatgtctgctcatggctcctcttcaatagagagttctccgcaccccaaatacagcggttcctctcctgaaggcgg
agcaccttacctgacaccctccagtgtcgcttccgaagggcctgtgtctcagttggcatcagaacaaagaggcctcgacgagacaccgacagtactggat
atagtcaccgagactgatgagtccatatccgacgaaggaagcgattttgaggaaactttgagtaaaagaccccggagagcatcaccgggagaaactgtgg
cagctaaacgtcccaggattgcagaggaaacggtgtgcgccaaggacttgtttgaggcaattagaatgggcaggagtgccatgcacgtactggttgacga
ttggttggatagttacaaacaggatcgtgaggcggctctgttagaactgatcaatttcctcatgctggcgtgtggctgtaatggtgtggtcactctggaa
atgctggaaaacatgcagaattctgacatcattcgcaagatgacaatggagtttaacgaggaaaccccagattacccgttgctcttaagcaccaagcctt
ggaagaaatttcatgctaattttggggatttctttaaagttctggtaaacagatgtcagtacaacatcatatatgatgagcttcttatggacgcactcat
ctctctgctcacggggctgtcggattctcaagtccgtgcatttcgccataccagcacatttgcagccgtgaagctaatgagcggcctggtgaaggtggca
tgggatgtcagtcatcatctaaacacgaccaagcgccagtttgttgtggaacatgcaaagagcccagagacgagatctccagagagactggaggccctgt
tggccaagcagagagggcttcaggggaatctggaagaggtcgtggatatgatgaatggtatttttaaaggagtttttgtgcatcgttactgtgacacgtc
tgcagacatccgagccatctgtatggaagagatcggggtttggatgagaacatacagccaatcatttctgagcgacagctgcctcaaatatgtcggatgg
actctctatgacaagcagggggcggtgaggctgcagtgcatccggacattgcacagtttatacagcgtcccagagatggccccaaagctggaacttttca
ctagcagattcaagaaccgtatggtattcatggtgttagataaagagcagcaggttgctgtagaggcaattaaactgattggactcatttcacagaacat
ggaggatatgctgagtaagaaggactgtgacactatttaccccttcgtgtttgtatccagtcgagcagtttcatctgcagcaggggcatttctgtatcag
agggttctagctcacacagtagctgaaacaagtcccaaaggcagaaatcggctcggaaatgtatccttctttcgactgctgatgtcattcttcataaaga
gtaagctccctgaacatgctgcgtatctggtagacagtttgtgggaatgcgctggggcccccctgcaagactgggtgtgccagactgatctgctgctgct
ggaagaggagggcttggatgacgcggatgaaagtgctctgatagaaatcttagtggcgtctatgagacaggcagtcgaggggacgtctcctgtgggtaga
gtgcccgctagaagggcttttgcattaaaggacaggaaggctgaagacaaaagccgattgacccgtcatatgattcttgcacttccccctctcttggcga
agttttcagctgatagcaagaaggtgagaactcttctgaagatcacttcctttatggagctggagatttactgtacagacagaatggaaaagtttctaga
tatgctgttggctgaggttcaggagatcttggagaagcatacagaggaagatgttttagagtcctgctctcgggctctgtacatcttgtgtgaccgcaag
caggccttgtaccagcgcgcagacattgcacgcagcaccttaatggaccggctcactggccgctttttgcagcaggttccggaaatcatgcaggagactg
agccagatgaagatgccgtttataatatcgctgcaacaatgaaaaggatatcaccattgtacagtggccatgatctgtctcgctgggagctgtttggtcc
atgcagccaaatactgctcaaggggatggacactggagaggttccagaacagatagtaatggctgctcttacctcctgccacttctctctcttgtgggag
cttttccatatctccaactccaaaggcccacagaatcagatttcccctctgaagcagaagctcaggcagttttgtgacatttgccaaggctgtctctctg
atctcaacgctgccatcaggaagcaggcatttgtcctcctcagtgatttgctggttatatttggcagtcccatagcccaggagcagagattttacctgca
gcctcttgcgtatcgtcctagtctgtctctgcaggctgagctggctggatttctgttggataatgtattcactgaccctgaagaagagaatgaggcccga
cgcactgaaacgctacacgagagaagaaacctgctggccggttactgcaaactgatcctgtatagttccctgcagctgcattctgccagcgatatcttta
ggcattatgtcaagtttaacgccagttacggggatctcattaaggagacgctgcacaagtgccgaagcatcagcagagaggagaccaccaagactgtgct
gctcagtctcactcaggcctttacagagctgtgccaagagggcgactccccaccccagcgctcctctcgccccttcctagaaattcgaggcttggccagg
agacttgctttgctctatggaccggatcagaggagatatcgccaggatattgttttgctgcacaaagagggcatcaaatactgtctgcgggagaataaaa
catctgattggagccctcagaacctgctcttcctggatgtgctgagtgaattctcccacaaaaccatcaaggaggacaagaaggcactcctgagatattt
agaagagaccactgagcggtgcctgccttcccaaccagtgaaagacaatgagatttgggcccctttcctggcgtacaggaagtccctgtgtgcagacgaa
gagtcccctcccagtaccgaaagcgatgtgtcgccgcgcagggtgggaacaaagaggagcactccgaggcctgaagtccctccccaaagcaagaggagga
gagggagcataggcacacttgatatttccagcctgatgatagaggaggggaaacctcgactgcccttaatgacttccacccagctaaaagaacaaaaaac
ccgcaccctccaggaggaagaaagatcggagacagactatgagcccagccaactcagtttcccgcgcatccagaggacgccaagctccaggccctgccca
caggctaggactccagcaacagtgatcagcaggctgagcttaatggaggaggaggaggaagacgaatacgtgatacaagaggatgagagcagcgagggtt
cagcagagtacaagccagatctgtttgattctgcactatatgaggaggagggcgtttaacttacagccgatatcccagagcgaagccaaacttcgggatt
actttgtgttagcgacccaagcgcagttgtttcctattcagttagccattaattaaacgtttgtttattctgcaaaaaaaaaaaaaaa
5' EST: CX916785 [+]cgtttatgttgacgtcgtaaaagtcggtaaaagggaacgttgttgcggaacaattatacgcttgtgaagtcgggacgcatttacgtgttcaggatgcatc
actcacaccagaaatcctcctcagttatgtctgctcatggctcctcttcaatagagagttctccgcaccccaaatacagcggttcctctcctgaaggcgg
agcaccttacctgacaccctccagtgtcgcttccgaagggcctgtgtctcagttggcatcagaacaaagaggcctcgacgagacaccgacagtactggat
atagtcaccgagactgatgagtccatatccgacgaaggaagcgattttgaggaaactttgagtaaaagaccccggagagcatcaccgggagaaactgtgg
cagctaaacgtcccaggattgcagaggaaacggtgtgcgccaaggacttgtttgaggcaattagaatgggcaggagtgccatgcacgtactggttgacga
ttggttggatagttacaaacaggatcgtgaggcggctctgttagaactgatcaatttcctcatgctggcgtgtggctgtaatggtgtggtcactctggaa
atgctggaaaacatgcagaattctgacatcattcgcaagatgacaatggagtttaacgaggaaaccccagattacccgttgctcttaagcaccaagcctt
ggaagaaatttcatgctaattttggggatttctttaaagttctggtaaacagatgtcagtacaacatcatatatgatgagcttcttatggacgcactcat
ctctctgctcacggggctgtcggattctcaagtccgtgcattttcgcataccagcacatttgcagccgtgaagctaatgagcggcctggtgaaggtggca
3' EST: CX916784 [+]tttttttttttttttgcagaataaacaaacgtttaattaatggctaactgaataggaaacaactgcgcttgggtcgctaacacaaagtaatcccgaagtt
tggcttcgctctgggatatcggctgtaagttaaacgccctcctcctcatatagtgcagaatcaaacagatctggcttgtactctgctgaaccctcgctgc
tctcatcctcttgtatcacgtattcgtcttcctcctcctcctccattaagctcagcctgctgatcactgttgctggagtcctagcctgtgggcagggcct
ggagcttggcgtcctctggatgcgcgggaaactgagttggctgggctcatagtctgtctccgatctttcttcctcctggagggtgcgggttttttgttct
tttagctgggtggaagtcattaagggcagtcgaggtttcccctcctctatcatcaggctggaaatatcaagtgtgcctatgctccctctcctcctcttgc
tttggggagggacttcaggcctcggagtgctcctctttgttcccaccctgcgcggcgacacatcgctttcggtactgggaggggactcttcgtctgcaca
cagggacttcctgtacgccaggaaaggggcccaaatctcattgtctttcactggttgggaaggcaggcaccgctcagtggtctcttctaaatatctcagg
agtgccttcttgtcctccttgatggttttgtgggagaattcactcagcacatccaggaagagcaggttctgagggctccaatcagatgttttattctccc
gcagacagtatttgatgccctctttgtgcagcaaaacaatatcctggcgatatctcctctg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: