XB-CLONE-3251870
Clone Name: IMAGE:7691711
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Gene:
kansl1l
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122934 [+]
AACATAGCAGAGCGGATGGAGGGCAAACAATTCTGCAGCCGAGACATCGCATTGATGAAGTCGCAGGGAGGAGGATGAATGAGGGAGTTGGGACCTATCA
GGAAAAAGAACCAATCAGAGGCACGTATGCTCGTTGCTGGATGTCTATTCTCTGTTCCAACGGAGGTCTGGCTGCACAGAGAGATAAGGACAGCCGGTGG
CTACCTAAGTTTCTTGAACAGCTGAGGTAGGGCTGGCTGAGGCTGCCTGTTAGAGATCACCCAAGCAAAAATGACCCCTGCTCTTACAGAAACAGTCCCA
CAGGGGCCTGGGGCTCACCTGGCACCATCCTTTGCCCTACAGAGTCTAAACTCTGACACTATTATTTGCATGGATAACTCCCAAGCTGTGGAGCATATCA
GCCTTCCGAAAGCCAATGGTCCCAAGGTTGCAGACAAAAAGCAGCTTCTCAAAACAGAATTTATGAATGGAAACAGCATTGGCTCTCAGACTTCAGGGCA
TTATCAAACAGTCTTTCTTTTGACTTCCAACACTGCTCTGAGTTTGCCAAATAAAGGCAGTTCTACATGGAAGATGGAAAATCAAGATGTTTGCAAGCAG
AGGTTTCATATAACGAAAGGCTCCCCCTCTCTGCCTAATGGTATGAGTTATCAGCCTGAAAGGAAAGCTAAGAGAGAACTTTTCCCTGGGTGCGTGAGCC
AGCTTTTAGCAGATGTGCACAAGCTCTGGGATGTATCTTTGACTAAGACTCAGGGTACTGAAGGAATCTGCTTACCAGAATGTATGTTAAATGGACATAG
AGGTACTGAATCTCTGGCTGTTAGCTCTTGCCCCTCACTTATTTCACATGTCTCTGCAAATGTACCCAGTGTTGCCTCCGTGAAGGCAGTAGGTACAAAT
TTGCTTTTGAAATGTCTCAACCATCAGCAAGTGCTCCTAAATCGGGCAAGAAGAAATCAGAAGCGTTTACAGTCTTATTTGGCACAGCATGCTGTACAGC
ATTTCAACCAACAAATAAGAGCCTTTGTGAATCACCAGATCCATGACAAGCCAGCTGGAATTTTTGATAGCCAACCAACCAAAGTGATCAACAATAATCT
AAATGTAGGGTCAAGTGGAATAAGTTCAGCAACAAGCGATGGGTTAAAAAATGGAGTTTATCATTCAGTGAAAAGGTTTTCAGTGTCTGCAAAGGAAATT
CTCAAACAGATTAAGAAAGATTTTGATTCTGATGCCACAGGGAGCAGTTCTGATGAAGACTGGGATGAAAAGGCTAGACAAAATTCGGATGAATGTAATG
CTGAAAGGAGCTGGCTTTCTGTCAGAACCAGAATTGGGAGTCGCTGGGTCTGGCTACAGTCTCAGATTTCTGAACTAGAGTACAAAATCCAGCATCTCAC
AGATCTTCATTCTCAGATACGCAAGGCAAAGGGGACACTGAAGTTTGAGGAACCCTCTAAAGGCAATTTTAAGCAGAAGCTGTGGCTGCCTGATTCAGAA
ATGTTATTAAGTCTTGCAGAGAGGATGCCAAAATCCCCACAGGGAACAAATCCGTCTCCAACAAATGATTTAGACATGTCACCAAGCAGTCCAACACTGC
TACTGAGGAACATAGAAAAGCAGAGTGCTCGGTTGACTGAAATAGTCAGCAGTCTAACAGTTCCACTTCTTTCTCCGAATGATTCTGCCAAATCTAGTGT
GTATAAAAGAGTGGCCAATGGATTCTCTGATAGGATGCATACTGATGGATTTCCTTCATTTAATGAATTCTGTGAGCAACAAGTGAAACGAAGGAAAAAA
GTTAGAGTAAAAGCCTCCTCTGCTTTGGGAAGCAATCTGTGCAGCTCAGCCAGAACCAGACCCCTGCAGGCATTGAAAAAACGGAAGCTCTACAAACTGA
GTGCAGAATATTCTTATGTAAATAAAGTGAATCTCTTCTCCTCACCAGTATACCAGTATGAAGAGCTGCCTTCTAACGGAAACCACTGCTCTACATGGAA
ATGTTCCAAGGTGCTGCACAGGCCCTGGTTAATGGCACAAAATGCCTGTGAGGCAGATTCATGCTTTCATCCTGTCTTGTCATTTCCTTATGAACTTCCG
CTAAATGTACACCTTGCTGCATTGCCAATGAAGAATCATAATATTAAGGGAAATTCAGTGGACTCCATAGCGCTCAGAGGGGAGCTGGATAAAAGCCAAT
CATATGTATCTGCCTCTTGGAGCACGGAATGCACGTCCAGTTATAGCCCTGACCCACAAACTCCTGCTCACAGCTGGGAAAGAAGGCACCGGAGTGAGTC
TGAAGCTGATGCAGCATTACTTGAGAGTGGCCTCACGACTCCAGTCTCCACTCAGAAATCCTCTGCCCAGCAACTTCTAGCGCATGAATCCAGCAGCATG
GTCTGTGCAGCGAGGCGCCGGTTAAGGAGTGGGAATTCTTATGACATCAACAACATTGTGATCCCAATGAGTCTTATTGCACCCACAAAACTGGAGAAAC
TGAAATACAAGGAAATCATTACTCCTAGCTGGAAGGAGGTGGTGCTAGAACCTTTGGAAAGTCCTGCTCATGATATGCCAGAAGACTTATCAGATGAGGC
CTACATTTCCCGTCATGAAAAATACGAGCTGAAAGAAAAGGCCCGGTGGTCATTGTGGGATCATAGCAAGCGGCCAAAGAGGAACAGATCATCAAGTTAT
AGCTTTGGCACCTCTCCAAGAACTGTGTTGCTTTCCTGTGAATGCAGCTGTAGCCCAAACTCTCAGGCTCCGTCAGAGGCTCTGCCTAGTGATACAGGAG
GCTATAGAACGCTTCATTTTTCCCATGAATCCCCAAAAGGAAAGACAATTCACTGGGAGCGCAGAGTCTTCCCATTGACAGAAGAACCTGCCATGGAGTT
ACTAGGCAAATGTCCTTCACAGGCCCAAGTTAGCACAGTGGACAATCAGCAGTGCCCGAAAAATGACTGTGACTACACATGACGGCCCTGTCTATAATCT
TTACTCCAACCCTGCTACAGAGATGGAGAAGTAAAAAGTAAGTGGAGCTGCAGAAGTAGTGCATCCAATTGATACACGAAAGTGATCTTCCAGCCAGTCT
GTTCCTAGTATTTCTGCTCATCATCAAGTGCCTGAAGAGTAATTTGGTTGTGCAAAGAGGGCAATACAATATCAGATCTGGGATGTACATGGCTACTCTT
GGTGTAGAGCTCCATTCTGCAAAGAAGACCACTGAAGCATTTTCAGAGTTATGGAACACAAATTAAGTTGATGTTGATTTTATTAGCAGCATGAGAATTT
TAGATTATTATAGTTTCCCTGCTTGGTTGCACAGGTACACAAACAGAAGTTCCCTTTCAGAGTCAATAATACAAAGAGTAAATGATTGTGTCAGATGAAG
TATTGCTCCATCACTTAGGCAGAGAAAATGAACAGAGCGTATTACTTGCAAGAACTCTAGTGCACATTTCTTTATTTCCCATATAGAGCACTCGTGGGGC
TACTTTCAGGTGCTTCTCTTTGAACACAATGGGAAAATATAATGATTCATTCAGGGTCTCGTGACACTGCAATCTGCAGGTTGTAGTACTGGCAGTTCAG
ATCTATATGGGACACCTGGCACCATGTTTAAAGGTGATGTAAACTTTTCTTTTTCCAAGCTTTTTCTGGAGCACTTGTATAACTCTTTTTTTCATGAAAA
AAAAATCCCATTTTATTGATCTCCTTTAGCTAGCTCATATTGCAGTCAAATGTCTGGGAGAGATGTCATTCACAAGTTGCTCTTTTTAAATTTGTTTTTT
CTTTAAATTAGATGAAAATTGTTCTGCAAATATTATTTTAACCTACTTCTAATTCAGTTTCAGCTACTATAAGTTTTAAAGGAAGTATCTGCAAGAAATA
GCACACCCATTGTTTTAATAAATTATATATATTTAATGAAAGGTAATACGACCCCCTTTAAGATATAGATGGGAAAATATTATTAGTCTTTTTAATAAGT
GCATGGCTTTATCCTACTCCCCAAGTTCTCCAGTGCCATCACCCCAGTTTCACTATAAATGCAAACAAGATGAAACTGCTGTCTGCTCAGTATGCACCCA
GACTGATGTCTTTTAAAGCCATGTTGTTCGCCGAGGTGGCCCACAGTGGGGGAGGAAAGCCAAGTGGTGTAATTGACATTTGGTACACTTCTGCCCTGCG
TCTGGCTGCACTCTGCACCCTGGGCTGACAGAGTGAACACAGGCAGCACTGTATTCATAGGGATGGCTTGTAGGTTGCTTTGCTCTAAAACAGAGTACAG
AGACCTTTGGCAATTTAGGAAGTTAGTGTTCAAGACAAACTGAGTGCAGGCTGCTTGTAAATGACCCCTAAGGTCACACAGGTGACACAGGAGCTAATAA
ATAGGCATTAGCAAATATGAACCATTTCTCCAAAATCTGGACCAGCAAGTATTCAAAAGACACACAGAAACCTTGTCTCTGTAATGTCCTGATTTTTTTA
TTTAAATAAAAATGTTATTCTCAAAAAAAAAAAAAAA
5' EST: CX916883 [+]aacatagcagagcggatggagggcaaacaattctgcagccgagacatcgcattgatgaagtcgcagggaggaggatgaatgagggagttgggacctatca
ggaaaaagaaccaatcagaggcacgtatgctcgttgctggatgtctattctctgttccaacggaggtctggctgcacagagagataaggacagccggtgg
ctacctaagtttcttgaacagctgaggtagggctggctgaggctgcctgttagagatcacccaagcaaaaatgacccctgctcttacagaaacagtccca
caggggcctggggctcacctggcaccatcctttgccctacagagtctaaactctgacactattatttgcatggataactcccaagctgtggagcatatca
gccttccgaaagccaatggtcccaaggttgcagacaaaaagcagcttctcaaaacagaatttatgaatggaaacagcattggctctcagacttcagggca
ttatcaaacagtctttcttttgacttccaacactgctctgagtttgccaaataaaggcagttctacatggaagatggaaaatcaagatgtttgcaagcag
aggtttcatataacgaaaggctccccctctctgcctaatggtatgagttatcagcctgaaaggaaagctaagagagaacttttccctgggtgcgtgagcc
agcttttagcagatgtgcacaagctctgggatgtatctttgactaagactcagggtactgaaggaatctgcttaccagaatgtatgttaaatggacatag
aggtactgaatctctggctngtagctcttgcccctcacttatttcacatgtct
3' EST: CX916882 [+]tttttttttttttttgagaataacatttttatttaaataaaaaaatcaggacattacagagacaaggtttctgtgtgtcttttgaatacttgctggtcca
gattttggagaaatggttcatatttgctaatgcctatttattagctcctgtgtcacctgtgtgaccttaggggtcatttacaagcagcctgcactcagtt
tgtcttgaacactaacttcctaaattgccaaaggtctctgtactctgttttagagcaaagcaacctacaagccatccctatgaatacagtgctgcctgtg
ttcactctgtcagcccagggtgcagagtgcagccagacgcagggcagaagtgtaccaaatgtcaattacaccacttggctttcctcccccactgtgggcc
acctcggcgaacaacatggctttaaaagacatcagtctgggtgcatactgagcagacagcagtttcatcttgtttgcatttatagtgaaactggggtgat
ggcactggagaacttggggagtaggataaagccatgcacttattaaaaagactaataatattttcccatctatatcttaaagggggtcgtattacctttc
attaaatatatataatttattaaaacaatgggtgtgctatttcttgcagatacttcctttaaaacttatagtagctgaaactgaattagaagtaggttaa
aataatatttgcagaacaattttcatctaatttaaagaaaaaacaaatttaaaaagagcaacttgtgaatgacatctctcccagacatttgactgcaata
tgagctagctaaaggagatcaataaaatgggattttttttcatgaaaaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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