XB-CLONE-3251776
Clone Name: IMAGE:7691639
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Gene:
cybc1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121946 [+]
GAGTAGAGCTGGGAGCACACAGATAGCCGGACAGGACCAGCCATGTATATGCAGGTAGAGAGCCGGACAGGAACCCTCTTGCACCTCAAGAGGAACCCCA
GCATACGGTCCTGGTCGCTCCTGGTGGGAATCTCCTCCGTGGGGCTGGCCGCTGCTTACTATAGCACAGATACTTGGCTGTGGAAGCTGTTTTATGTGGC
TGGCTGTGCTTTTGTGGCTCTTCAGAACTTGGAAGACTGGGAGGAAGCCATTTTTGATAAAAAGAGTGGAAAGGCCATTCTGATTACGTACAGCTTATAC
AAGAAACTACTGACACTCTGCAAAGGAGGACAGGAACAAGTGGTGGTGCTCCTGAAGGAGATCAGAGACGTCAATGTTGAAGAAGAGAGAGTGCGTTACT
TCGGGAGCGGTTACGTGATCGTCCTGCGCTTTGTAACCGGAATCTCTCACCCATTGACGCAGAGCGCGGTGCTGGGGGCTAGAAGTGATGTTGAAGCCGT
GGCTAAAGAACTGACCAAATTTTTGGAATTTGACCTTGTGGGGTCCCGGCCACAAGCAGTGGAAGAGAGCAATGACAGTGAAAGCGATGAAGCCCTGGAC
ACACAATAACTCTCCGCCGGCTGATCTGTGTATATTGAGTAAGGGCTTGTTATACCATAGGGCATTCCTGTGACCATCGGGAGCTAGACTAGAACATTCT
TTGCATCTGATGACATCACATAGTCTGGGTGACAGGAAGTGCTGTAGCTGTGACATCATCATTGGACCTATAACATCACACACTGAACCATTCCAGGACT
GCGGAAGAGTAATGACAGTTAATGACATTGTGGTCCAACCATTAATCTTCCCATGTTGCACACCCAGGCCTGTACTTAGCAAACCGGCCAGCTGGCTGTG
ACTTTCTCTTACCGGCTACCAATAGAATTAGTGCAGAGCGTTTGCCTATAGGTGCATGTAGTAAGAGGCACGCCTGCACTATTCTAATTGGTGACTGGGA
ACAGCAGGGGGTGGGGACGGACAGCTGATCCAGTTCAGTGCTGATTCGCACAAATTGATATTTTTGCCCGATGAGGGAAATTCAGTCGCTTTTGTCTTGC
TTTTTATGTAGCAAAATTGTTCCGCTTGGCATAGAGAATGCTTGTAGCATTTATTCATTTTGCCTTTACTTCCCCTTTAATGGCTGGTGCATGGAGCCTT
AACATCCAATATCTTGCTGACAATTGGGCAGCTTAGCCAATCCCGCTGATGCCTGCATCGCCTCTTGATCCCCTCCTCTCCCGACAGTTCCACACAGGCT
AACATTGTCATTCCGTCTTTGGCCCAAGGGCCAAGTATCTGATGAAGCTGATTCGGCCCAGTACCCAGGAGGGCAAATTAGACAAATTCAGATGCCAAAG
AGCAGATCTCAAGTCTGTTTATCTTTTCCCCTAGACGGTCAGTGTAGTGCAGGGTCCCTTACCTGCCGGTGGGATAGAATCATGGGACCAGGGCTAGAAT
GGCTGAATACCAACTGAACCGTACCAAGGTGAATGGTCACATTCTTCCATATAACTGCTTGGCTGGGTGACTGCGGGGACCTGACCCAGAAGGATTGGGT
ACCGGCTCTTCTAATTAGTGTAATTCTTCATCTTACTGAGCCGTTATTGTTTAATAGAAACCCACAAAGGGAGGGATGGACGCAAAGTTAGCTGTCCCCG
ACTTACTCATTGCAGTGGCTTCTGCCCATGGTACTGACTTTGCAACTAGCTATTACACTTAGGTACTATTTAAAATATAATATGTAAGAGAGAAAATTTT
GCACTCTGTCTCATCATCATTCCAGTATTCAGCTAAAACGAAATACTCCATCCAAACTTGCAGTGTTTCTACTTACTTTTAGTTTCTTTCAGATAATGGT
TTGGCTGGGGGCCTTCGGGGGGGACCATTCCCATAAGAATTTAATGCGGGCTGTGTTAAAAACACAATGATATGTACAATAGTGGTTTATCTTTTACATT
GTGCAATAGAAGGGATATATATAAACCCTGCTGCAAGCTGGGAAACGCCAAAAGCCCCGCCCATCTTCCTCATTGGGTCGGCTCCTGGCCATGGTACTGA
CAGATGGAACCTGCTTCCTACCTTCTTAACTACCTGTTACACTCCGGTACTATTAATGTATCTGTATAATATACAGTTTGGTCAACTGTAAATTATATCT
TAGTGATGTCATCAGTTATAATCGGAGCTTAGTGATATCATTTCTGTCACGTGACTCACTAAAACTTGTATATAATAATATAGTGCCCCCTGTTGTAAAA
TATGAGGATATTAGAAGTCACCTCGGAATTCCATGATCTCGGCCTTCAGCCTTGTACCTTTATATGGTCATGGAACATATAAACTCCCCATATATAACTC
CCCAGTGGCTTATAATACCCCTTTATTGCTCCCCTAAACCCGCAGTCTATCCACTTATTTTTATTGTATTTCATTCAAATAAAAGCAGTTTTTCTATAAA
TAAAATAAAGTCCTTGGGAACCAATCTACCTGGGGACATAAGGAGAACCTGAGACACTTTCGATGTTGCAAAACTACAACTCACTACACTTTGTAGTGCA
GACATCAGCTTTATCTGCTAATAAGCCTCACTCCCACCATTTATTCTCTCTGTATCCCAGCAGCCTTCCCATTTTAACGTTGCCTTAAATCAGTGTTTCC
ATTTGGGGGGGGGGGGGGGTTGTTAAGCTTCTGCTGTATTTAATTCAATGTATGTAAAGGCCAAATCATCCCGGTGTATACTGCAGCGTGTGCCTTTTTT
ATGTACTTATAATTGACTTTTTCCAAGGCTTTGGGGAATAATTAGCAACAGGAAAGAAGGCTATTCCAGCAGCAACCAATCAGTAGGTTTCTTTCATTAC
TCTCTATTAACTGTACTAAACGGCTCATTGGTTACTATGGGTTACCAACTGCCTGTTGAACGATACGCCTCTTTTTAAATTACTTGAAATAATATTTACA
TGAATTTTAGCTGTAGGACATACTCTGTTTTTATACAATGAATAAAATGGTTTAAAATGCAAAAAAAAAAAAAAAA
5' EST: CX917057 [+]tagagctgggagcacacagatagccggacaggaccagccatgtatatgcaggtagagagccggacaggaaccctcttgcacctcaagaggaaccccagca
tacggtcctggtcgctcctggtgggaatctcctccgtggggctggccgctgcttactatagcacagatacttggctgtggaagctgttttatgtggctgg
ctgtgcttttgtggctcttcagaacttggaagactgggaggaagccatttttgataaaaagagtggaaaggccattctgattacgtacagcttatacaag
aaactactgacactctgcaaaggaggacaggaacaagtggtggtgctcctgaaggagatcagagacgtcaatgttgaagaagagagagtgcgttacttcg
ggagcggttacgtgatcgtcctgcgctttgtaaccggaatctctcacccattgacgcagagcgcggtgctgggggctagaagtgatgttgaagccgtggc
taaagaactgaccaaatttttggaatttgaccttgtggggtcccggccacaagcagtggaagagagcaatgacagtgaaagcgatgaagccctggacaca
caataactctccgccggctgatctgtgtatattgagtaagggcttgttataccatagggcattcctgtgaccatcgggagctagactagaacattctttg
catctgatgacatcacatagtctgggtgacaggaagtgctgtagctgtgacatcatcattggacctataacatcacacactgaaccattccaggactgcg
gaagagtaatgacagttaatgacattgtggtccaaccattaaatctccatgttgcacac
3' EST: CX917056 [+]ttttttttttttttttgcattttaaaccattttattcattgtataaaaacagagtatgtcctacagctaaaattcatgtaaatattatttcaagtaattt
aaaaagaggcgtatcgttcaacaggcagttggtaacccatagtaaccaatgagccgtttagtacagttaatagagagtaatgaaagaaacctactgattg
gttgctgctggaatagccttctttcctgttgctaattattccccaaagccttggaaaaagtcaattataagtacataaaaaaggcacacgctgcagtata
caccgggatgatttggcctttacatacattgaattaaatacagcagaagcttaacaacccccccccccccccaaatggaaacactgatttaaggcaacgt
taaaatgggaaggctgctgggatacaaaaagaataaatggtgggagtgaggcttattagcagataaagctgatgtctgcactacaaagtgtagtgagttg
tagttttgcaacatcgaaagtgtctcaggttctccttatgtccccaggtagattggttcccaaggactttattttatttatagaaaaactgcttttattt
gaatgaaatacaataaaaataagtggatagactgcgggtttaggggagcaataaaggggtattataagccactggggagttatatatggggagtttatat
gttccatgaccatataaaggtacaaggctgaaggccgagatcatggaat
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: