XB-CLONE-3251694
Clone Name: IMAGE:7691426
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Gene:
elp1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121944 [+]
CAGAATTAAGGCCCTGGAATGAGAAATCTCAAGTTAAAAAGGTGTTTGCGCTTCAACACAAGTGGAAGTTTAGGCTCCCCACAATGCAGCGCCATTCGGT
CAGAAACTGGAACGGTGCTAATTGGATCAGGCTATGGTTTTGTGGAATTTGACCCGAATTCTCAAGAGATCATCAAAGACATATCTTTAACAGCTGCGAA
TTTCCTCCCAGAGGATGGAAATGGCCATCTTGTTGGAATCCAGGATGTCCCTGATCAAGAATCAGTATGTGTTGCAACATCTACAGGTGATGTCCTGCTA
TCTAATATTAACACTGGCCAGTTGGAGTGTGTTGGCAGTGTGGATAGTGGCATTCTTGCAATGAGTTGGAGTCCAGATCAAGAACTTGTTCTTCTTGTCA
CAGGTCAACAGACTCTAATCTTAATGACCAAAGACTTTGAGCCAATTGCTGAAAAATCAATCTACCAAGATGATTTTGGTGAAGAAAAATTTATCACTCT
TGGGTGGGGCAAAAAAAGAAACGCAGTTCCATGGGTCCGAAGGAAAAAAAGCAGCTCAACAAAAAATAATGGCAGTACAGCCTGCTTTGCCGTGGGACGA
CCATAAACCAAGAATCACATGGAGAGGAGATGGACAGCTGTTTGCTGTTAGCTCAGTTTGCAAAGAGACAGGTAATCGTAAGATTCGAGTGTGGAATAGG
GAACTTGCACTGCAGTCTACCAGCGAATCTATTGAAGGCTTGGAGCAAGCATTGTCCTGGAAGCCTTCAGGAGCTCTAATTGCCTCATCACAGAGCAAAC
CCAATAAAAACAGCATTATTTTTATTGAAAAAAATGGTCTTGTACATGGAGAGTTTACACTTCCATTCATTAAAGGCCAAAAAAAAAGGTGAAAGAACTT
CTTTGGAATTCTGACTCCACAGTTTTGGCCATCTGGCTTGAAGATGTTGAAAAAGAAGGGTCCAGCTCAAGCTGTTGTGTTCAGCTCTGGACAGTTGGAA
ACTACCACTGGTATCTTAAACAGAGTTTACATTTTGGAAATAATAAAATGAATAATGTAGAGTGTTTGATGTGGGACCCTGAGAATGCATACAGACTCCA
TATCATCTGCACAGGCTGGCATTACTTTTGTTTTGACTGGTTCTGGAGCACAGACTGCAGTGACGGAGGGCAGGGTGATGTGGCTGTAATTGATGGAGAT
AAAGTTTTAGTGACATCATTTCAACACGCTGTGGTCCCTCCTCCAATGAGCACATACTTTATTCAGTTATCATGTGCAGTAAATGAAGTTGCATTTCAGC
TTGATCCCAAGGAAAGCAGTGGTCTTGCAATCCTTGATTCTGCTAATACCCTAAGCATTTACAGATATGGGAACAGCACAGTTAATGATCCAACAGTAAA
ATTAGAAGCAGTGGGGGGCAATGGATTTAAAACATCATACAAGACTCCAAAACTAGAGAAAAAACTTAGATTGCCAAGTAATTTATGTGATGTGCAGCCA
CTGAACTTCCGCCTCCTTACATGGGTACAGGATGATACATTCATAGCTATTAGCCAGGAGCCCAATTCTTCCATATCAATTGTCCACCAAATGACTGCTG
ATCAAATGGATGGTCAGGATATTAATGTCAGGGACGTAGGAACAATAACTGGACATGTAATAAGTCTCTGTTATAGCTCAAATACAAAGCACTGTGCTAT
ACAGACATCAAATGGACAGTTATGGAAATATCTCTGGGAAAACCCCACTCCAGTTGTAGAGCCCTGGATTGATGGTATGGGCCATAAAGTGAAATTTCCC
CAACCTTGTCTACAGACTGCATTAACAAGCATTGAAGGAGAGGAAGTGGTAATAGGTTTGACGGAGAGATCACGCCTCTTTATCAATAGTTCAGAGGTTG
CAAGCAATATCACTTCATTTCACCTGTATAAGGAGTTTCTTCTATTAACAACCCACTCACATACATGTAGATGTGTGTCGCTTCAAGACACTTCACTAAA
AGCACTGGAAGCACAGCTTAACAGCACAAGTAACCCAAATGATGAAACCATTCGCAAAGTGGAAAGAGGATCTCGAATTGTCACAGTAGTGCCATTTGAC
ACAAAGCTTATCTTACAGATGCCTAGGGGAAATTTGGAAACCGTACACCATCGAGCACTGGTGTTGGCTCAGATTCGGAAGTGGCTCGACAGCCTTTTAT
TTAAAGAAGCCTTTGAATGTATGCGTAAACTCAGAATCAACCTCAATTTACTTTATGACCACAATCCAAAGGCATTTTTGGACAATGTGGATCTTTTCAT
AACACAGATAGGCTCTGTAAATTACATCAATTTGTTTTTGACAGAGATAAAAGATGAAGATGTAACAAAGACTATGTATGCAACAGCTTCTCTTATCACT
ATGCAGTCCTCTCAAGGAGCTAAGGCTAAAAAAGTTGACATCATTTGTGATGCTGTGAGGACAGCCATGGAAAAGCTGGATCCTCAGAAATTTTGCTTAT
CAATTCTCACATCTTATGTCCGGAAAACAAAACCAGAGTTGGAAATAGCACTCCAAAAGGTTCATGAGCTAAGAGAGAGCCCTTCAACTACAGTTGTCAG
TGCTGATGAAGCCTTGAAGTACTTACTGTTCTTGGTTGATGTAAATGAACTTTATGATTATTCCCTGGGGACATATGATTTTGATCTAGTTGTGATGGTG
GCAGAAAAATCACAGAAGGATCCCAAAGAGTATCTTCCATTTTTGAACAAGCTGAAGAAAATGGAAATTAATTATCAGAGATACACCATTGACAAACATT
TAAAAAGATACAGAAAAGCACTTAGAAATCTTAGCAAATGTGGATCAGGTTATTTCGCTGAGTTCTTGAACTTGGTTAAAGATCAAAATTTATATACTGA
AGCCTTAGAACTTTATCAGCATGGAACTGTTGAATATAAGGCCATTAATGCTGCTTATGGGGATCATCTTGTTTCTAAACAGCAGTATGAACTAGCAGGC
TTGATATATGCCCGCTGTGATAGCACTGAGAAGGCACTTGATGCTTTTGTTGCAAGTAGCAACTGGCATCAGGTAATGTGTATGGCATCTCAACTGGAAT
ACTCTGGGGAAAAAATAGCTGCCCTAGCTAGAACTGTTGCTGGTAAACTTTTGGAGCAGAGGAAGCAAGCAGATGCAGCTGTACTTCTAGAGCAATATGC
TGAGGACTATGAAGAAGCAATACTGTTATTATTAGATGGTGCACACTGGGAAGAGGCTTTGCGCTTGATCTACAAATACAAGCGCCTTGATATACTGGAA
ACAAATTTAAAACCAGCACTCTTAGATGCTCAAAGAAATCACATGATACTCTTTGAAAACCAGAAAACCACATTTACTCGCCACAAGGAGCGCCTTTCTG
TGGTACGAGAAATGAAGGAGAAAGCCCGCCAGGGTTTGCTGGATGAAGATATGACTGGTTGTGCTGAAGCTGACCTTTTCTCTGAAACCAGCAGTATTAT
GACTGCAAGTAACGCGAGTGGCAAATATTCTCAAAGCAACTCAAGGATTTCTTCGAGGTCCTCAAAGAACCGTCGAAAAGCTGAAAGAAAGAAGCACAGC
CTGAAGGAAGGAAGTCCACTTGAAGACTTAGCATTGCTTGAGGCTCTAGCAGAAAACATTAAAATGGTGGATAAATTAAGAGGAGATGTACATAGTCTTT
TAAAAGTGCTTATTCTCTTTGAGTATGACACAATGGCCAAAGAACTTCAGCAGGCTTATGATGATTTGCTTCAGCTATTTGAGACATCAATTCTGGATAT
TTGGCCCCCAGGTGTACAACAAAATACTGCGGCACCAATTCTAGGTCCTCATTCAACTGCAAACAGTATTTCAATTTCCTATCAACACCAAAGGAATGTA
AACACTTCAGTGCAAGATTCAGAACTGTTTACTGCTCCGAAACTTAGTAAAAACATCCAATGGAAACTCTGTGTGCTTCAGCAGTATGAAGATTTTCTAT
AAATAAATTATAACTAAAGATACAAACAGGTTTCTTCTCAATGTACTGCCTTAATAAAGATTTATTTGCTCTACAAAAAAAAAAAAAAA
5' EST: CX917238 [+]cagaattaaggccctggaatgagaaatctcaagttaaaaaggtgtttgcgcttcaacacaagtggaagtttaggctccccacaatgcagcgccattcggt
cagaaactggaacggtgctaattggatcaggctatggttttgtggaatttgacccgaattctcaagagatcatcaaagacatatctttaacagctgcgaa
tttcctcccagaggatggaaatggccatcttgttggaatccaggatgtccctgatcaagaatcagtatgtgttgcaacatctacaggtgatgtcctgcta
tctaatattaacactggccagttggagtgtgttggcagtgtggatagtggcattcttgcaatgagttggagtccagatcaagaacttgttcttcttgtca
caggtcaacagactctaatcttaatgaccaaagactttgagccaattgctgaaaaatcaatctaccaagatgattttggtgaagaaaaatttatcactct
tgggtggggcaaaaaaagaaacgcagttccatgggtccgaaggaaaaaaagcagctcaacaaaaaataatggcagtacagcctgctttgccgtgggacga
ccataaaccaagaatcacatggagaggagatggacagctgtttgctgttagctcagtttgcaaagagacaggtaatcgtaagattcgagtgtggaatagg
gaacttgcactgcagtctaccagcgaatctattgaaagcttggagcaagcattgtcctggaagcctttcaggagctctaattgcctcatcacagagc
3' EST: CX917237 [+]tttttttttttttttgtagagcaaataaatctttattaaggcaggacattgtgaagaaacctgtttgtatctttagttataatttatttatagaaaatct
tcatactgctgaagcacacagagtttccattggatgtttttactaagtttcggagcagtaaacagttctgaatcttgcactgaagtgtttacattccttt
ggtgttgataggaaattgaaatactgtttgcagttgaatgaggacctagaattggtgccgcagtattttgttgtacacctgggggccaaatatccagaat
tgatgtctcaaatagctgaagcaaatcatcataagcctgctgaagttctttggccattgtgtcatactcaaagagaataagcacttttaaaagactatgt
acatctcctcttaatttatccaccattttaatgttttctgctagagcctcaagcaatgctaagtcttcaagtggacttccttccttcaggctgtgcttct
ttctttcagcttttcgacggttctttgaggacctcgaagaaatccttgagttgctttgagaatatttgccactcgcgttacttgcagtcataatactgct
ggtttcagagaaaatgtcagcttcagcacaaccagtcatatcttcgtccagc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: