XB-CLONE-3251676
Clone Name: IMAGE:7691624
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Gene:
iqsec1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC155699 [+]
GGAGTGACGGGCCGTTATCTGCCCAGCCGGAAAGCGCTGTAAGGTTCCGCCCATCCGGTACCGGCTGCTCCTCGTCGCTCGGCCTCCTCCCCGGGGGGAT
AAAGCTGTCAGTACGGAGCATCCTCTGCGCCGCGGAGAGGGAGGGGAGGCCAGAATCCCCGGAGCCCGGAGTGGTGTTGAGGGGGACGTTCCAGGGAATG
AGAGCGGCGGCTCGTTAGACGGCACCAGTTACCACCAAGGGGCAATTACACACAGCTCATCGCTGAGTCCGGATCAGTACGATCACCCCCCGTACGGCAT
TTACCCAGTGACTCCCGGCCAGAGGTCCCGCCGGCCCAAGCTCCAACACTCCACCTCCATATTGCGCAAACAGGCCGAGGAGGACGCCATTAAAAGGTCG
CGCTCGTTATCGGAGAGCTATGAGTTGTCATCAGATCTACAGGACAAGCAGGTGGAAATGCTGGAGCGCAAATATGGAGGCCGTCTCGTAAGCAGACATG
CCGCCCGCACCATCCAGACCGCGTTCCGCCAGTACCAGATGAACAAGAACTTTGAGCGGCTGAGAAGTTCCATGTCGGAGAATCGCATGTCGCGGCGCAT
CGTTCTCTCCAACATGAGGATGCAGTTTTCCTTCGAGGGCCCCGAGAAAGTCCACAGCTCGTATTTCGAGGGGAAACAGGTGTCGCTGAGCAACGACGGC
CCCAAAATGGGGCGCATGGTTCAGTCCGAATGCGGAGACCGCGGCGAGCAAACGGCCATGAAGTCGCCGGCGGCCTCCAGTGACTTTGCGGACGCCATTA
CGGAGCTGGAGGACGCTTTCTCGCGGCAGGTCAAGTCCCTGGCGGAGTCCATAGACGACGCTTTAAACTGCCGCAGCCTCCACGCGGACGAGGGCCAATC
GGCCGATCCGATCAGGGGGCGGGAGATGGAGCGCAGCAGTTCGGGGCAGCTGAAAGCGGCGCATAATCTCGACCACAGGAAACTGGACGAAATGACGGCG
TCCTACAGCGACGTGACTCTGTACATCGACGAGGAAGAGCTGTCCCCTCCTCTCCCTCTCTCCCAGTCCGGAGACAGAACCCCGAGCACAGAATCCGAAC
TGAGACTCAGGTCTATGAACTCTCAGGAGTACTGGTCCATGACGCACAAGGAGGACAAGTTGGACACGGACACCAGCTGTAGGAGCACCCCCTCTCTGGA
CTGCCAGGACCACCGGTCGCGGATCGACCACCTACCGCTGCTCACCATCGAGCCGCCCAGCGACAGTTCCGTGGATCTCAGCGACCGCTCGGAACGAGGG
TCCGTAAAGAGGCAAAATGTGTACGAGAGGGGGATCCCCAATCAGCAGGGGAGCCCCAAGCACATCCCCCATATCCCCACTAAGATTTTACCCCGCGAGG
AGGAGCAGCCCCGCCACAGGCCCCGCCCCATAGACGCCCAATTGATCATTAACGGCACCATCAACCGGCAGAGTAAGTCGGAATCGGACTATTCCGACGG
CGACAACGACAGTATCAACAGCACGTCCAACTCCAACGACACCATCAACTGCAGCTCCGAGTCGTCCCGCGACAGCCTACGGGAACAGACGCTCAGCAAG
CAGACCTATCACAAGGAGACGCGCAACAGCTGGGATTCTCCGGCCTTCAGCAACGATGTCATCAGGAAACGCCACTATCGGATTGGCTTAAACCTATTCA
ACAAGAAGCCGGAGAAGGGGATCCAGTACCTGATAGAGAGGGGGTTCGTGCCGGACACGCCGGTCGGGGTGGCACATTTTCTGCTGCAGAGGAAGGGGCT
GAGCCGGCAGATGATCGGAGAATTTCTGGGGAACCGGCAGAAGCAGTTCAACAGGGACGTCCTGGACTGTGTGGTGGATGAAATGGACTTTTCAGCAATG
GAACTGGATGAAGCTCTCAGGAAATTCCAGGCTCACATTCGGGTGCAGGGGGAGGCGCAGAAGGTGGAGCGTTTGATTGAAGCCTTTAGCCAACGATACT
GCATCTGTAACCCCGGGGTTGTCCGGCAATTCCGCAACCCCGACACCATCTTCATCTTAGCGTTCGCCATCATCCTCCTCAATACGGACATGTACAGCCC
CAACGTCAAGCCCGAGCGCAAGATGAAATTGGAGGATTTTGTGAAAAACTTACGAGGGGTAGATGACGGAGAGGACATCCCGCGCGAAACTCTGGTTGGG
ATCTATGAACGGATCCACAAACGGGAGCTGAAGACAAATGAGGATCACGTGTCGCAGGTGCAGAAAGTGGAAAAACTCATCGTAGGCAAAAAGCCGATCG
GATCCTTGCACCATGGACTTGGTTGTGTTCTTTCGTTACCGCACCGCAGGCTCGTCTGCTACTGTCGGCTTTTTGAGGTTCCAGATCCAAATAAACCACA
GAAACTGGGTCTGCACCAGCGGGAAATCTTCCTGTTTAATGACCTGCTTGTGGTCACAAAGATTTTCCAGAAAAAGAAGAATTCTGTAACTTACAGTTTC
CGGCAGTCGTTTTCCCTTTATGGAATGCAAGTTCTTCTGTATGAGAATCATAATTACCCTAACGCCATTCGTCTCAGCTCGGCCATCCCTGGCGCAGACG
TTAAGGTTCTCATCAACTTCAACGCCCCAAATACTCAGGACCGTAAGAAATTCACGGATGACTTGCGAGAGTCGATAGCCGAAGTTCAAGAGATGGAAAA
GTACAGAATAGAGTCGGAGTTAGAAAAGCAAAAGGGAGTGGTGCGGCCCAGCATGTCCCAGAGCTCCAGCCTGAAGAAGGATTCGGGCAACGGCGCCCTG
AACAGGGCGTGTCTAGACGACAGCTACGCCACGGGGGAGGGGCTGAAACGCAGCGCCCTAAGCAGCTCTCTCAGGGACCTCTCGGAAGCAGGCAAGCGTG
GGAGACGCAGCAGTGCAGGATCGCTAGACAGCAATATGGAAGGGTCCATCATTAGCAGCCCCCACATACGCCGACGAACCCCATCTACCCGGGAGTGCCC
GTCTCGCCAGAGCATGCCCAACTCTTCCTCCTCCCTCTTAGGCTCTTTATTTGGCACAAAACGGGGCAAACCCCCATCCTCCTCCTCGTCCCAAGGCCAC
CTGGCTTCCGGCGCCTCGCAGCACCCGACTCTGATCTCCCACCAACGCCACTCAACGGGGGAACTGGTGGGGCCGCCGGAGGGCCAAACCCAGGCACAAG
TACACGCACAGCACTCCCAGTACTACCATATGCAGCAGAACCCTCCTCCCTACCATCATCATCATCACTATCTCCCACCCCAGCACCTTCAGCAGCACCA
GTACCACCCCCTGGCACAGCACCCCCCCTACATGCAACATTCCCACCTCCCCCCGACCCACTCTGCACATTCCCAGCATGCACAGCACCACCACAGCCAG
CAGCTCTCCTCCTCATCCGTAAGCACGAAACCAAAACACAGCGGCATCAGTACAATAGTCTAGGCCCCACAATGCATTGCTCCCGGCGGAAGCGAATCCG
GCCGACGCGTTAACGTTTACATAGTTAGTGGGAGAGACGTTAATGGTGGGTTGGGCAGAGGGGCCCCCCAGCAGGGCCCACAGAACCTCCGTAGATAGAA
CCAAGAACATATTCTACCGTTTAACACTTTAGAGCCGAATCACGTGGCCCCTCTCGTCCCACTGTGGCCCTGCATTAACGCTACGAATAAGTGTCATGTT
TTAGGGGCGGGGAATCAAATCGTATTTATTGCTTGATAACCGAGTAAGTGTACAAATAGACCAACTGCATCGTTGCTGTGTAACTTCCCCCCTGCTTTGC
TTCCCGGAGCTGCTAGGGTTAATATGAGATTAGGATTTAGCGGATTATTCTACTTGAACCCGAAACACCTTTTGTTCTGACTTCGAGCAGTTGTAGCAAT
ATGGGGGCGGAGCCTGGACGGAACGCACTACTGCTGGGACATTTGCACTTAATCCACAAATAAATGTGCCAACCCCTCACTCACACTCACTCTGCTTCAC
TCACACTCACCCCCCTGACATTGGATCAGTAATCGCCCCCCCCCAAACCGCTCTCTCATGCCCCTCCCCCTTATTTATTTATCATTTTAATTAAAAAGTA
ACATTTTTAAGTTAAAAAAAAAAAAAAAA
5' EST: CX917288 [+]gagtgacgggccgttatctgcccagccggaaagcgctgtaaggttccgcccatccggtaccggctgctcctcgtcgctcggcctcctccccggggggata
aagctgtcagtacggagcatcctctgcgccgcggagagggaggggaggccagaatccccggagcccggagtggtgttgagggggacgttccagggaatga
gagcggcggctcgttagacggcaccagttaccaccaaggggcaattacacacagctcatcgctgagtccggatcagtacgatcaccccccgtacggcatt
tacccagtgactcccggccagaggtcccgccggcccaagctccaacactccacctccatattgcgcaaacaggccgaggaggacgccattaaaaggtcgc
gctcgttatcggagagctatgagttgtcatcagatctacaggacaagcaggtggaaatgctggagcgcaaatatggaggccgtctcgtaagcagacatgc
cgcccgcaccatccagaccgcgttccgccagtaccagatgaacaagaactttgagcggctgagaagttccatgtcggagaatcgcatgtcgcggcgcatc
gttctctccaacatgaggatgcagttttccttcgagggccccgagaaagtccacagctcgtatttcgaggggaaacaggtgtcgctgagcaacgacggcc
ccaaaatggggcgcatggttcagtccgaatgcggagaccgcggcgagcaaacggccatgaagtcgccggcggcctccagtgactttgcggacgccattac
ggagctggaggacgctttctcgcggc
3' EST: CX917287 [+]ttttttttttttttttaacttaaaaatgttactttttaattaaaatgataaataaataagggggaggggcatgagagagcggtttgggggggggcgatta
ctgatccaatgtcaggggggtgagtgtgagtgaagcagagtgagtgtgagtgaggggttggcacatttatttgtggattaagtgcaaatgtcccagcagt
agtgcgttccgtccaggctccgcccccatattgctacaactgctcgaagtcagaacaaaaggtgtttcgggttcaagtagaataatccgctaaatcctaa
tctcatattaaccctagcagctccgggaagcaaagcaggggggaagttacacagcaacgatgcagttggtctatttgtacacttactcggttatcaagca
ataaatacgatttgattccccgcccctaaaacatgacacttattcgtagcgttaatgcagggccacagtgggacgagaggggccacgtgattcggctcta
aagtgttaaacggtagaatatgttcttggttctatctacggaggttctgtgggccctgctggggggcccctctgcccaacccaccattaacgtctctccc
actaactatgtaaacgttaacgcgtcggccggattcgcttccgccgggagcaatgcattgtggggcctagactattgtactgatgccgctgtgtt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: