XB-CLONE-3251581
Clone Name: IMAGE:7692075
|
Gene:
enpp4
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC121205 [+]
AACAGAGGAAAAATCAGTGTGACAACTCGGAGCCTGAAACTTATCGGTGGGACTGTCCCCGCCGCCCGCAGGCACATCCCCGGCAGTGTAGGAGCCCCTG
TGTCCCCCGCCATGGCCACCCTGTAAGGCAGTACCGGCTCCGTGAGTTTCACCTCTCAGGTAACAGCAAGGAATCGTCACATCAAGATGTTGGGAAGGGC
TTTCATTGTGGCTGTATTGTACTGTGCGACGTTCTGTAAGGCAGATAAACCTACTAACAGTTCCACTCCCAGGTTAATCATCCTATCCTTCGATGGATTC
CGGGCTGACTACCTAACAAGATTCCCCATGCCTAACCTTGATGAGTTTATGAAGGAAGGGGTTCAAGTGGAAGAAGTCAAAAATGTCTTCATAACCAAGA
CTTTTCCCAACCATTACTCCCTAGTCACCGGCCTTTATGCCGAAAGCCACGGCATCGTGGCCAATCACATGTATGATAATGACACTCGGCAGGTATTCCA
TATGAACGATTCAAATTCCAAGTGGTGGGACGAGGCAACTCCTATCTGGGTGACAAATCAGAAGCAAGGCCATAAAAGCGGATGTGCCATGTGGCCCGGG
TGTAATGTGCCCATGCACAATGTAACCCTCAATGCATCTCTTAATTATAATCCCAATGTTACCTTTACTGAAAGAGTAAATAACGTCACCATGTGGCTTA
CGAGGCCCAATGATCCAATCAACTTTGCCACTATTTACTGGGAGGAGCCTGATGCAAGCGGACACCGTTTTGGGCCCGATGACCATGACAATATGGCCAA
AGTACTGAAAGAAGTGGACGAGCACGTGGGCTACTTGATGTCAGAGCTAAAAAAGGCCAAGCTTTGGGATACAGTCAATGTGATTATCACTAGTGACCAT
GGCATGGCCCAGTGCTCCAAGGACAGAATTATAAAACTGAATGACTGCATCGGCCCCGGAAACTATACGCTGGTGGACGACAATCCTGTTGCAGCAATCC
TTCCACTTTCAGATACACAGCTTGTCTATGATCTGCTAAGGAACTGCCACCCTCACATGAAGGTGTACAGGAAAGAAGAGATCCCAGAGCGCTGGCACTA
TAAACACAACAGCCGGATCCAGCCGCTGCTTCTGGTTGCAGATGAGGGATGGATGATTACCCAGAACCATTCAATCTCTATGTTGGGAGATCATGGCTAC
GACAACGACCTTCACAGCATGCACCCCTTCCTGGCCGCCCACGGGCCGGCATTTCGCAAGGGTTACAGAATGCGCACCATAAACAGCGTGGATATCTATC
CCTTAATGTGCCACATCCTTGGCATAACCGGGCTGCCCAACAACGGGACACTGAAGGATATCAAATGCTTATTGGTCAACCAGTGCTACATTCAAATCCC
GGAAGCTATCGGAATAGTCATCGGAGCCATCATGGTGCTCACCACCCTGACGTGCATCATCGTCATGCTGAAGAAGAAGATGCCCTCCACGCGCCAGTTT
TCACGGCTTCAGTTTCAGGATGATGACGATCCTCTGATTGGTTGAAGCTAAAGATTTCCTACTACCAGATTGTAATGTGCTTAATGCTTCTGATGGTGGC
CAAGGTAGACGGGTTTTGGGGTGTGGAAGAATGCTGACCCTCTTGTGTGTAGGGATATTCGGGTACACTCTGTGTGTCACCATCTCCAGATTAACCATTT
GGTTTCCATTGAATTCTGTGCCACATACAGGCATACAGCTACGGCGGCACACTGTCCGTATGTATGTGCCAACCTCCCCGGCACAGAGACCTCCGGCTGC
GACTAAGCAATATTGGTTGCTGATTGGATAGAACTGATCGACTACCCGGAGATAACGACAGTCCTTTATTACTGGAAGAATGAAAGCCCCAATTGTGTAG
CCTTATTGCACTTTGCCGGTGGGAGTCGCTTCTGATTGGCTTGTGCTTTCCATGATTTTAGGAACTAAAGAAAATAACAGCACATAGCGAAACCTTTCCC
TGCTTGCATTACTGGGGCACAGATTGGGGAAAATACTTGAATTGTACTTTCTTATTTAAATATTGCCTTTTCTTCCGTCTTGCCTTTGTTTAAAGCAATA
TTATTCCTTCCTTCCGACGCCCTGTATTTAGGGTTGTTTGTTATCATAGGTTGTTTCCAAAATTGCACTATGTGAGCTTCTGCCTCCGTATGATCCATGG
ATCTTCCATCTATGGTATATTATTGGTTTGTCTGCACTGAGCCGTAGCACTCGCTTCTATGGCCGCTTGCCCTTTAAAGAGACAGCGCCCCGTATTTCTC
CATTTTCATATGCCAAGTGTGGTTCATCTTTGGCGCTTTACTTGACTTTTTATTGTCACGCAGGGTTAATGTTGATCTTTTGCTCGCGTAACAACCATCA
AATTCTCATTTTCCATAAATTTGACATTTGTTCTATTTGTAAGTGATACTTAGGGAAGAAAGTCATTTGTATTCTTTGCATTTCACAGTCCTTACAGGGT
TGCTTTAAAATGAAGTTTTAGTATAGACCATAGTAATCCAAATAGGATTAGTCTTTATATTTGGGTATTTTCTTTATTGTTGTACAGTCCTGAGGCTTTA
AAGGCAAACTTTCTGGTTGCTAGGTCCTTAGTTACCCTGGCAATGTGGCAGCAGTTTGAATGTCAGACTGGAAGATGAAAAGTACAGTTCCTACATAAAA
ACATTTAGTGAAAAAATAAATGAGGGAGATGTATTCCTTTTCAAATGAGTTTACAAATGTTTAAACTCACTGGACTATTGAACCCCCTCCCCTATACTGT
ATGGAACTTTTTTTTTGATTACTCTCAAAAATGAGTTTGGAATTAAAAAAAAAAAAAAA
5' EST: CX917436 [+]aacagaggaaaaatcagtgtgacaactcggagcctgaaacttatcggtgggactgtccccgccgcccgcaggcacatccccggcagtgtaggagcccctg
tgtcccccgccatggccaccctgtaaggcagtaccggctccgtgagtttcacctctcaggtaacagcaaggaatcgtcacatcaagatgttgggaagggc
tttcattgtggctgtattgtactgtgcgacgttctgtaaggcagataaacctactaacagttccactcccaggttaatcatcctatccttcgatggattc
cgggctgactacctaacaagattccccatgcctaaccttgatgagtttatgaaggaaggggttcaagtggaagaagtcaaaaatgtcttcataaccaaga
cttttcccaaccattactccctagtcaccggcctttatgccgaaagccacggcatcgtggccaatcacatgtatgataatgacactcggcaggtattcca
tatgaacgattcaaattccaagtggtgggacgaggcaactcctatctgggtgacaaatcagaagcaaggccataaaagcggatgtgccatgtggcccggg
tgtaatgtgcccatgcacaatgtaaccctcaatgcatctcttaattataatcccaatgttacctttactgaaagagtaaataacgtcaccatgtggctta
cgaggcccaatgatccaatcaactttgccactatttactgngaggagccctgatgcagcggacaccgttttgggcccgatgaccatgancatatggccaa
agtactgaaagaagtggacgagcacgtgggctacttgatgtc
3' EST: CX917435 [+]ccaaactcatttttgagagtaatcaaaaaaaaagttccatacagtataggggagggggttcaatagtccagtgagtttaaacatttgtaaactcatttga
aaaggaatacatctccctcatttattttttcactaaatgtttttatgtaggaactgtacttttcatcttccagtctgacattcaaactgctgccacattg
ccagggtaactaaggacctagcaaccagaaagtttgcctttaaagcctcaggactgtacaacaataaagaaaatacccaaatataaagactaatcctatt
tggattactatggtctatactaaaacttcattttaaagcaaccctgtaaggactgtgaaatgcaaagaatacaaatgactttcttccctaagtatcactt
acaaatagaacaaatgtcaaatttatggaaaatgagaatttgatggttgttacgcgagcaaaagatcaacattaaccctgcgtgacaataaaaagtcaag
taaagcgccaaagatgaaccacacttggcatatgaaaatggagaaatacggggcgctgtctctttaaagggcaagcggccatagaagcgagtgctacggc
tcagtgcagacaaaccaataatataccatagatggaagatccatggatcatacggaggcagaagctcacatagtgcaattttggaaacaacctatgataa
caaacaaccctaaatacagggcgtcggaaggaaggaataatattgctttaacaaaggcaagacggaagaaaggncatatttaaataagaaagtacaattc
aagtattttccccaatctgtgccccagtaatgcaagcaggg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: