XB-CLONE-3251379
Clone Name: IMAGE:7691840
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Gene:
ccdc39
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121424 [+]
ATTGGAACCGGGAGGTTGAACTGAAGCGCTCTGATACCGGGAGCCCCGCTCTCTCTCACCCGTTACACTTGGCGTTTCGCTTATAGGCGCCTGTTCCCGT
TACAATGAACCGTAAGGTGCTGGACGAGCTTAGATGGGACGATGGCTATGCCATTCCCGTGGCCAATGCGGAGAATAAAACCCTGGAGGAAGAGATGCAG
AAGAAAGAAAAGGAAAAGATCCAATTGTCGAACCAAGTGAAGGGATTCGAAGAACGAATCCAAGCAATGGCAGACCATCTCAGGAATGTCAGACAGGAGT
TGACATACACTCAATCCCTCGTAAGGGCCAGGGAAAATGAGACAGAGAGTGAAGAACACTTCAAGACACTGTCAGAAAGGGAAATTGGACGTCTCAAACA
AGAAATTCAAAGGCTTGAAAATGAGCTAGTGTCCTTGAGAGAAAAGAAAAACAGCCATGAAAACAACATATTCAAAACCACTCAAAAGCTGGAAAATCTT
AAGCGTCAGCTTAACTGGGACCAGCAGACTTTGGAAGCTTGGCTCGAAGAATCTGCACGCAAAGATGAAGATTCCATGGCAATACAAAAATATTCCCAGA
AAGATGATGGAAAAATTAAAGAACTGTCACTGCTGATTGAAAAACTGACTCTGGAGGCTGGTCAGAAACGTAAACTTATGGACAATGAGTTGACAGAGAC
TATCACTGCACAGATAGAGCTGGACAAAACAGCTGAAGATTTTCGGAAAGCTCATACTGAAAGACAGCAACTGATTAGTCAGTGGGAAAGCACCATAGGA
CAAATGCAGAAAAGGGACAGAGACATTGATCACTGTGCAATGTTACTAGCCCAGGTCAAACAGGAGCTAAGAGAAAAAGAAAGTATAATCAAAGAGAAAA
TTCAGTTCCTGCAAAGTGAAATTGACAATAATAAGGAATATGAGAAAAAGATTTCTGCTGCAGAACGACAGGCTGCAAAGCTGAGATTGGAGCTGCAGGA
ACAAGAGATCATCCGAGGTCGTCTGCAAGATGAGCTGGATTCTTTAAAAGGTGTTGTTGATCGGACAGCTAGTGACTTAGAGACTATGAGAACAGAGGTT
ACCAGTCTAAAGAAAGACATCCGGAATAGGCAAAACAGGCTACAAATGGCACAAGAATATAATGATGGCCTATCTGACAAGCTTAACTTTGTCACTGAAT
CAGCCTTAAGTCTGGAAGAAAGAGCTTTACGGATGGAAGAAATGTTCAAGGAACAAGAAAAAACTGTTAAGGATCTTGAAGTCCAGTTAAAACAGATAAG
GGAAATACTTTTCAAAAAATCTCAGGAGCTGCATGAGTGTAAAGCAAAAGAAAAGGACGTCATAGCAGAAATCAATGGAGGCCAAGTTACACTCAAGAAT
CTCAATAGTCAACTACAAAAACTTGACTCACATGCTCTAAAGCAGCAAGGAATAATGTACAATCAGGATTTTCAGATCCAGCAATTAGAGAGACGGCTAT
CACGTTTAAAAGGTGAGGTCAATACAGATGAGAAGCAAGCCTTGGAAATGAGGGTATCGCAGCTTACTAAAGTATTTGAGGAGCGCAGAGCTGCATTAAA
ACTATTGAATACCCAGCACAAGAAACTCCAGGGGGATGTGCGTTATATCAAAAGGGAACTTGAAAAAACTGGCCAGGAAAAGGGAGATTTGATCAGCAAA
AAGGAAGAATTTAATCTCTTCAATATGATGTCTGAAAAGACTCTGAAAAAGATCAAAGCATCTAAGCAGGATTTAATGGTTGAAGACAATATTTTAAAGC
TGGAAATAAAAAGGCTAAGGGACATGCTCTACAGTAAGGCAGATGATGTTTTCTCACTGGAAAAACGCAAGTTGCAGTTACAAACTGCAATGAAAGAACG
AACCGAAGAGATTTCAGTCCACAAAGAAATGCTTAAATCACATATAAGATACGTGGATCAGGAACGCCAAAATATAAGTTCACAGCTTCATGAGAGACTT
GCAAAAGTTGAGAAAATGAGGAAGAGGTTTGAAATACTAACTATTGCAATGATGCCACCAGAGGGTGAAGAAGAGAAATCTCAGGCCTATTACGTAGTTA
AGGCTGCTCAGGAAAAGGAGGAGCTACAGCGAGTAGGTGATGATCTGGATGGGAAAATTCGTAAAGCTGAAAAAGAGATCAGGGCTTTAGAAAACACCCT
GCGAGTTATTAATGGGTGTAATACCTCTTTCAAAAAATCATTTTCCAAAATCACAGAAACAAGTGAAGAATACGACGAAAAAGTTCAACTTGAAGAAGAA
AAAAAAGCTGCAGAAGAAAAGCATCGATACAAACAGAGGCAGATTAGGGAACTGCAGGAAGATACGCAGGTTATGAAAAACACATTTGATAGCATGATAA
ATGAGGAACTGTCCTATCAAGAGAAAGCAAAGGAACTGCTTCCAGCCATTACCCAACTTAACAAAGAAACAGAGGACCAAAAGCAAAAACTGGAAAGAGT
ATTGAAACAGTATTCCAAATTGTCAAAACATGTCCGAAATGCTAAGAAAAGCAAAGAAGAAACACAAGAGGAAAAAGATATTAATGTTCGGGATCTGCGA
GACTTCAACCAATCCTTAAACAAGATGCTGGCTCAAGCAATGGAGAAGAATCCGGACTTAGCCACAGCCCTTCAGTTATACTTTCATCAGTTTGGTTTAG
AAATACCTACAATTGGGTCAACTCCTGGAAGTTACAGTTCTAGATCAAGTTCATCACGTAGTTCCACTATTTCCGTCAGATCAGGCACATCCAGGATATC
AGTCAATTCTGGTCAATCTCCCCGGATTAAAGTAGTGGACCTAGGACTGCCTCCTGCCTGTCCTTCATCAAATGTATCATCTGCCCGGAGCTCCAGGACA
TCATCCAGGAGTGGCTCGTCAAAAAGCCAGAAACAGGAGTAAAATGTATTTATGTACTAATTATTATTTAGAGAAAAACTTCTCATTTAATTACTTATCT
ATATAATAGCATACATGATTTATTTTTCACCACCAAAACAGTAGTTAGTGAGAAATATAAGGACAGAAGACAAACTAGGAGAGGGAAGATAACAAGAAAG
GGACTGAAAGTAGAACATAAAAACCAAAAAGATTTCAAAAAAAAAAAAAAA
5' EST: CX917812 [+]attggaaccgggaggttgaactgaagcgctctgataccgggagccccgctctctctcacccgttacacttggcgtttcgcttataggcgcctgttcccgt
tacaatgaaccgtaaggtgctggacgagcttagatgggacgatggctatgccattcccgtggccaatgcggagaataaaaccctggaggaagagatgcag
aagaaagaaaaggaaaagatccaattgtcgaaccaagtgaagggattcgaagaacgaatccaagcaatggcagaccatctcaggaatgtcagacaggagt
tgacatacactcaatccctcgtaagggccagggaaaatgagacagagagtgaagaacacttcaagacactgtcagaaagggaaattggacgtctcaaaca
agaaattcaaaggcttgaaaatgagctagtgtccttgagagaaaagaaaaacagccatgaaaacaacatattcaaaaccactcaaaagctggaaaatctt
aagcgtcagcttaactgggaccagcagactttggaagcttggctcgaagaatctgcacgcaaagatgaagattccatggcaatacaaaaatattcccaga
aagatgatggaaaaattaaagaactgtcactgctgattgaaaaactgactctggaggctggtcagaaacgtaaacttatggacaatgagttgacagagac
tatcactgcacagatagagctggacaaaacagctgaagattttcggaaagctcatactgaaagacagcaactgattagtcagtgggaaagcaccatagng
acaatgcagaaaagggacagagacattgatcactgtg
3' EST: CX917811 [+]tttttttttttttttgaaatctttttggtttttatgttctactttcagtccctttcttgttatcttccctctcctagtttgtcttctgtccttatatttc
tcactaactactgttttggtggtgaaaaataaatcatgtatgctattatatagataagtaattaaatgagaagtttttctctaaataataattagtacat
aaatacattttactcctgtttctggctttttgacgagccactcctggatgatgtcctggagctccgggcagatgatacatttgatgaaggacaggcagga
ggcagtcctaggtccactactttaatccggggagattgaccagaattgactgatatcctggatgtgcctgatctgacggaaatagtggaactacgtgatg
aacttgatctagaactgtaacttccaggagttgacccaattgtaggtatttctaaaccaaactgatgaaagtataactgaagggctgtggctaagtccgg
attcttctccattgcttgagccagcatcttgtttaaggattggttgaagtctcgcagatcccgaacattaatatctttttcctcttgtgtttcttctttg
cttttcttagcatttcggacatgttttgacaatttggaatactgtttcaatactctttccagtttttgcttttggtcctctgtttctttgttaagttggg
taatggctggaagcagttcctttgctttctcttgataggacagttcctcatttatcatgctatcaaatgtgtttttcataacctgcgtatcttcctgcag
ttccctaatctgcctctgtttgtatcgatgcttttcttctgcagc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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