XB-CLONE-3251179
Clone Name: IMAGE:7692177
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Gene:
asxl2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121991 [+]
CAACATGGCAGCGGGCAGCTGAGCGCGGCAACACCGACTCGCAGCGACTCCGAGCGGCACCCAAGCCGGGACATGAGGGACGCTGCACGGAGGAAGAGGA
GGAGGCGGCGGAGGAAGAAGAAGGGACGGACATGGGCTGAGGCCGCCAAGACGGTCCTGGAGAAATATCACAACACCCCAATGAGCCATAAGGAGATCCT
ACAAGTCATCCAGCGCGAAGGCCTGAAAGAGATCAGCGGCACGTCCCCCCTCGCCTGCCTGAACGCCATGTTGCACACTAATTCCCGGGGAGAAGAGGGA
ATATTCTACAAGGTTCCGGGGAGGATGGGAGTCTATACACTTAAGTCGCATCTAGAATATCCCAGCCATCCTCTCCCCAATCAGGCTGTCCATCTCCCTC
CATCCCTGCGGGGAAAGTCATCTCTCCATCTCAGAAGCACAGCAAGAAGGCCCTCAAACAGGCTTTGAAGCAGCAACAGCAAAGGAAGAAGCAGCAGCAG
AGGGTGGGAATGCCAGTCCCTGCCAGCCAGCGCCTCCTGCTGACTACAGTGAAGACTGCAAACAATGTTGCCCCTGCCAAGCCAACCCCAACCCACACAT
GGGAAGCCAAACAGCCAGAGGGCCAAGCAAGCAATGCCCACATCTCTGCGCCATCCGGCACCTCCCTGGCATCTCTACATGGACTGGGGAAGAAGGCATT
GCCGAGATCAGACCGACTGCAAGCCAGACATATAAAAAGAACAAAATGCGCAGAGATCGACGTAGAGACACCGGACTCCATCTTGGTCAATACCAACCTA
CGGGCACTGATAAACAAACACACCTTCTCACTCCTGCCCGGGGACTGTCAGCAACGGCTCCTCCTCCTGCTGCCTGAAGTGGACCGACCAACTGGTACTG
ACGGCCTGATGAAGCTCAACAGTTCCGCCCTTAACAACGAGTTCTTTACCTCGGCGTCCCAGAGCTGGAAGGAGCGGCTCTCAGAAGGGGAATTCACCCC
AGAGATGCAGCTTCGGATCCGCCAGGAAATTGAGAAAGAGAAGAAGGTGGAGTCGTGGAAGGAGCGCTTCTATGAGAGTTACTATGGGGAGAACTCTGGG
CTAAACCCTGACGATTACAAAGAGCTGATTGCAGAGACCAACGATACTTTGGCCAGAACCCAAGTCTCCCCTCCAAAGGAACAGTGTAAGTCCGACCCGT
GCCCTGAACTCCAGCCGGTTGAGGCCAAGGTTGAGACCACAGCCCAGAAGGGTATAAATGAAGGCCCACCAATAAAGAAGGAAGATGAAGGCACTGTTCA
TGTAAAGGCCGAATTACCAACAGCCGGTGATACCCAGCGCCCCCCCGAAGTCATGCCAGCGGATACCAAAGAGAAAGTCAGCACTGTTCCTGTGAAGACA
GCAGAGGGCGCTGCAGAGGTTATAACCCCAGTTCCCAGCAAAGAGACTGGGCATAAAATATCCCCTAAGCCGGAGGAGACAGACTCAGAGAGCCAGGGTA
CACCAACCCCAACCCCACTAACCAGTCCTGTCCATATGCAGCCTCCCAGCACTCCCACAGCCAAGGCTGCGCCGGCTCAGGGTTCCGGGGCAGAGGGGTC
GCCAGAGAAAGTGGAGCTCGCAATCGGCACACGAGTCAAGAGGAAGCTGGATGTGGGCGCCGGGGATTCGGATAATGCTGGGAAATGCATACGGATATCG
GAGCAGCAAGAAATCCAGCGGCCATTTCGTGACTTGTGTAGCCCCAGTACCAAAGGGACGGCCCAGGGGTCGGAACAAAAAGTGCCCCCACTTAAGATCC
CTGTCTCTTGGTTACCTACAAAGCCCTTTCCCAGCAGCCGGGTCTCTTCCAGGCCCTATTTTCCAACCACCGGCACCAGTCCCAGCAGAACAGGAGCAAG
AACCTTGGCAGACATCAAAGCCAAAGCCCAGCTGGCCAGAGCCCAGAGGGCAGCAGCGGCGGCAGCGGCAGCAGCAACATCAGCTGGCCTCTCTGTCGGT
TCATCAGTGCCAGGGCCAGGGCCTGGAGGGGGAACCGGACCTGGAGGAGGAGCCGGACCTGGAGGAGGAGCCAGACCTGGAGGAGAGGCGAGGAATCCAG
CAGCTGGCACCAGTGAAACAGGACACAGGGCCGGAGTACTGGACGTGGGAAGTACTGGAAGCTGGAGAGGTGAGAGGGGAAATCCAGCCCTTCCATCATG
CACCCAGACTTCCAGGGAGACCCAGAACCCACTGCAGGTATCACCTGACAGTACAGCAAGAACACAGCTACTGCAAAAATCCAGTCTACAACCCAGACAC
CCAATAACAGGAGCCCAGGCATCTCTAGCCTATGGCTGTCCCTTACCTGCCCGGAGCAGTGCCAGCATCAGAATGCCCAATCCGTTATCTGATAGACTGG
TGGCAACATCTGGCACTGTGGCCCCTGGGGTTACAGCTATACCTCCATCAAGCACAAGCTGCTCATCACCACAGAACCTAAAAGGTATCCGGGCCAACCT
AGCTGCTCAAATCAGACCTAGAATTTCTTTAGGCACTCAGACAATCCCACCAAGGCCTACGGAGACAGTCCCACCAAAGCCTGCCGAGACAATCCCACCA
AAGCCTGCCGAGACAATCCCACCAAGGCCTGCCGAGACAATCCCACCAAGGCCTGCCGAGACAATCCCACCAAGGCCTGCCGAGACAATCCCACCAAGGC
CTGCCGAGACAATCCCACCAAGGCCTGCCGAGACAATCCCACCAAGGCCTGCCGAGACAATCCCACCAAGGCCAGCTGAGACAATCCCACCAAGGCCAGC
TGAGACAATCCCACCAAGGCCTGTCGACACAATCCCACCAAGGCCTGTCCTGTCTCAGGCAGTAACTTCTAACCTTTCCACACCAGCCAAAGCTCAGGCA
CCCAGTCAGCCAGCAGGTCCCATTCCAAGCACTCAGAGGGTGCCCATAAGGACGGGCTCTAGCATACCCGCCAATAACCCTCTGGTTACCCAACTCCTGC
AGGGCAAGAGTGTCCCCATGGAGCAGATAATGCCCAGACCTCTCAACAAGATGGAAGTAAAGACCATTCCTATAGGTTCTGGTCAGAAGGAGAGGGTAAC
TAACACAGCTGGAGTTAGTGCCCCTGCAGGGGGCATCATGGTAAGTAGAGAAGATTCAGAAAAGCCCTCACACATGGCTGTCCAACAGCTGGAAAGGTTT
CTTAGCCAAAATCGGCAGCTGCCGTCCAGCCAAAGGATCCTGCAGCTCTTCTCTGGAAGAGATCTGAGCAGTATTAGCCAATCACAGAGCCAGGAAGCAG
CCAGCAGTACCGCCCAAGAGCAAACTCTCCAATCACTGATCAGAAAGGTTCAACAAGAGAAATCATTGGTCGGCCCCAATCCAGCTGAACCGAGTGAAAA
CACATCCGCCAGTCAGAGGTTCATGTTTGGCTTTGTGGGCCGGCGAACGTCTAAACCTGCCATGTCGGGGCACTACTTGCTAAACATCGCTACGTACGGC
CGCGTTCCCGAGAGCTTCCGGCGAGCACAGGCTGTAAGTCCTGAGCGGGGCATCAACCTGAGTGACCCAGAGAGGTCTCAACCCAGAGACGATGAAACAG
AATCCGTTACTGAGAGCGAAGAAGAAGAAAACAGTGAGAACAATCCAGAGGATAGTGAATATGCCCCCCGATATCTCCCCATAGCCGTAAGTCACCCCAC
CACACTCAGGACTGCAAAACTCGAGGCCCCCATGTTAGATCTGAAAGAAACGGTCCAGGCTTTATCAAGGGGGGACATTTATGATGAGTCCCACATAGCC
AGAGACTTTATCCAGGCCGCCCAGGCCAAGATGGTGAATGTATTGGGCATAAAGCTGAAAGGTGATGCTTCTGAGCTGTTCAGGGCGAGCAAGTCCCACG
AGGGCTCCCAGCCGCCCCATTCTGAGCAGATACACACGGGCGGGCCCGCCGGCCTCATCGGATCCAGTTACGGAGGAACCATCAATATCACCACTTCGCC
CGAGGGAATGCAGGGGCCCTCCATCCCTACAGGGGGCCATCTCACACCCACCAGCGCTGACAATCTGGTCTCCTTCTCTGTCACCGTCACAACCATCCCA
TCCAGCCACATCGTAAACTCAAGTGATCACGACCAACCCGTTTCTGTCCAGGCCTTCACCGAGGACCCCGGCGGGGACACGGCCCCTTCCAAATGCTACT
GTCGACTGAAAGCCATGATCATTTGTAAAGGCTGTGGCGCCTTCTGCCACGACGACTGCATTGGGCCCTCGAAACTCTGCGTCTCCTGCCTGGTGGTGCG
GTGACGGCAAACCCACGGCTACGATCTCATTGGCCGAGAAGGAAGGACACAGAGGGTACTCTAATGGATCAACAATGTTGACACGTTTGCACTTATGAAA
TCCTGTATATAATATATATATATATATAAAAAAAAAAAAAAA
5' EST: CX918182 [+]caacatggcagcgggcagctgagcgcggcaacaccgactcgcagcgactccgagcggcacccaagccgggacatgagggacgctgcacggaggaagagga
ggaggcggcggaggaagaagaagggacggacatgggctgaggccgccaagacggtcctggagaaatatcacaacaccccaatgagccataaggagatcct
acaagtcatccagcgcgaaggcctgaaagagatcagcggcacgtcccccctcgcctgcctgaacgccatgttgcacactaattcccggggagaagaggga
atattctacaaggttccggggaggatgggagtctatacacttaagtcgcatctagaatatcccagccatcctctccccaatcaggctgtccatctccctc
catccctgcggggaaagtcatctctccatctcagaagcacagcaagaaggccctcaaacaggctttgaagcagcaacagcaaaggaagaagcagcagcag
agggtgggaatgccagtccctgccagccagcgcctcctgctgactacagtgaagactgcaaacaatgttgcccctgccaagccaaccccaacccacacat
gggaagccaaacagccagagggccaagcaagcaatgcccacatctctgcgccatccggcacctccctggcatctctacatggactggggaagaaggcatt
gccgagatcagaccgactgcaagccagacatataanaagaacaaaatgcgcagagatcgacgtagagacaccggactccatcttggtcaatacaacctac
gggcactgataaacaaacacaccttctcactcctgcccggggactgtcagncacggctcctcctcctgctngctgaagtggaccgaccnactggtactga
cggcctgatgaagctcaacagttc
3' EST: CX918181 [+]atatatatatattatatacaggatttcataagtgcaaacgtgtcaacattgttgatccattagagtaccctctgtgtccttccttctcggccaatgagat
cgtagccgtgggtttgccgtcaccgcaccaccaggcaggagacgcagagtttcgagggcccaatgcagtcgtcgtggcagaaggcgccacagcctttaca
aatgatcatggctttcagtcgacagtagcatttggaaggggccgtgtccccgccggggtcctcggtgaaggcctggacagaaacgggttggtcgtgatca
cttgagtttacgatgtggctggatgggatggttgtgacggtgacagagaaggagaccagattgtcagcgctggtgggtgtgagatggccccctgtaggga
tggagggcccctgcattccctcgggcgaagtggtgatattgatggttcctccgtaactggatccgatgaggccggcgggcccgcccgtgtgtatctgctc
agaatggggcggctgggagccctcgtgggacttgctcgccctgaacagctcagaagcatcacctttcagctttatgcccaatacattcaccatcttggcc
tgggcggcctggataaagtctctggctatgtgggactcatcataaatgtccccccttgataaagcctggaccgtttctttcagatctaacatgggggcct
cgagttttgcagtcctgagtgtggtgnggtgacttacggctatggggagatatcggggggcatattcactatcctctggattgttctcactgttttcttc
ttcttcgctctcagtaacggattctgttt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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