XB-CLONE-3250971
Clone Name: IMAGE:7692228
|
Gene:
nedd4l
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX918561 [+]
tacttctccaagtcactccatctgcacactgggctgccgctggtctccacaatggctccctgtgaggactaaataagatgactctttgggaggctactca
atcagaaaggcttctagccgacaagagatgattttttgggccaagttgatgttccgctgaatcacttaccgacagaagatccaacaatggaaaggccata
cacattcaaagacttcttactcagaccaagaagccacaaatcccgggttaagggtttcttgagactgaagatggcctacttgccaaagaatggaatgcag
gaagaggagaataatgagcaaagagaagaatccgaggcctgggatgtggtggactccaatgattcaagctctccacatcagcaagagctcccagctccac
ctatgccgccgggatgggaagaaaaagtggacaaccttgggcggacatattacgtcaaccataataacaagaccactcagtggcaaaggccaagccttgt
agacgttacgtccgaatcggacagcaacattcggcacatacagcaagaggcccaccgagtgttccgatctcggagacatatcagtgaagacctagaaccc
gaccatatagaaggaggagcggatgttccagagccatgggaaaccatttccgaggaaatgactttgacgaccgacacgttaaaccagtctctcccacccc
ctgcttccccggattctcgggccagcgctctggaactgtccgaggagctgaacaggaggctgcagattagct
3' EST: CX918560 [+]tttttttttttttttgagtatatagctttaattaaattgaaataaaatctgaaaataggtctaaatcattttaaaaagtaaacatgaggtttaatactat
acatgtgaagtgattagttccattcgttagaaagttatgtaaaatatgtatcaagggctttttggcctaacgaggcaccacataaatagagcaagtcctc
ctgaaatgtagtctaacgtgtaccccggggcagaacccatccaccgccattgcaaacgcctgaaatgtgcgcctgcgctgcagaaccaatatcaggggcc
gtttgccaaagaccagagaatggcttgcatattggcacagtttgaaccattagtctgcctgcccgggccgattcacaaaggaaaatggtaccatggttgc
cggtaagcagggcgctgttcccacgcgtcccgcggtcagctcaatggacacaacgtcataaatggcagaatcttttgtcccatttttgtaattccgttta
tttgcaattgccccttgtgtgtcagtggtacaatccgtagcacaagttcccggtgcaagccgcgctttagctggagagagacagaggcctctttagtcga
cgccttcgaatccctgagcgttctccacggccatgagaagcttctcccgtaaatcctcaaacgagtcgtatggaggtaagtccaggcggttaaagcatgt
gtgagctctgggcagcttgtcggggcttccccactgctctattgtaaacagctgaggaccatttgaaccgtacagttcagcaaatccattcataggcaca
cgtgacgtcccagtcacaaactgcagcaagcgtatgcgtttctctgcatccatta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: