XB-CLONE-3250961
Clone Name: IMAGE:7692374
|
Gene:
pacsin2
,
mxi1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX918582 [+]
tgacccccactcggcagcaacagcaataaatacccagaatgaatagctgggatttagggtggacatgtgagcagaaggggcctgggcagaattagcataa
acaacacccccccaattgaataggtgctatgaacactgctgtctggttgccagggtcactgaccacagcaacatagcatctgaccgactgtcagcccagt
ctgcccagtacaggggtcacaaagtgagatggttctgtccctgttagtataaagtgtgtgtattttcctgttccaaatccgcccggtcccacttgtgact
gtctcgcatgtgactgctgctgaactgcaacctgccgtcacctgaccgcatcacttacgccgccgccgggcagttacatatttgtatttgttcctgacaa
gaatcttctgtagttctactatttgcctactcagggttttattcctagctgtatccctgggttattggggtagtggggcattctgggtaattgtctgcat
aagaggctgggatccaaataggtgagagagcaagtgagagagaggtgaggcagggcctggaaatcaatagctccctatcttactgattgtgtgcccagaa
cattccctctctgtatatttgaatttatacatatgggtagggggtgccatagtgtttcccttagacagtacagtatgggggtacagcttattgtgtgccc
agaacattccttctctgtatatttgtatttatacatatgggtaggaggtgccatagtgtttcccttagacagtacagtatgggggtacagcttattgtg
3' EST: CX918581 [+]gtacatctcttcccagtgaatagattacaggtattgccgagtactaagcgtgctctaggggcggggcatggggaggtgggcagggccgtcgctcctgtca
ctgcaccgactccacgtagttggctgggtacaaacccacttggcccccgtccaggcggcccttgcaccagccctgttcgtcctcgtcttcgattttcgtt
agctcctcccctgctttaaagctcagctcgtccagttcctgcccctcgtaatcatacagggccctcactcgcacctccgtggcccccggcgcatcttcat
cgaatgggttgctgtcgccgttggcatcgttggccgtgaaagggttgttggattcatcgtcggaccactccgcggggtaggttttatcgtagctgcccac
gctgctgtgcttgttctgtacgggcgcctgctcgcccgcttggctgattccggtcagggtgaccccgtccgccggcttcttcttctctctcctgctgagg
gtgcggttcagatcagccgaccactcctcaaactggggccagttcatagacattcccgggccgttgttgttccggaaccatttcagatcctcggtggcat
cggccgacttgatggcgtattccagctctcggtagatattggcatagctgtctacgttggacagatcgaggtgcttctccacctccagcagaacctcccg
gaagaagcgcagacgtttatcctcaaactgctggcactgctcgaacacctgctccatgttctccatgtactgcggggtggtgccgtccaggtccttcagg
gccttctcatacttctctttg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: