XB-CLONE-3250791
Clone Name: IMAGE:7692845
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Gene:
als2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC123947 [+]
TGTCAGACAGGCAACAAGGAGCTTTCAATTAACAGGATTGCGGGACGTGTTACTGGTAGAACAACATCTACACTAGAGATTTTAAACTATAATTATCTTC
CAAGGAGGTGTGCTACAGGAATAAATCACTGACTGATGATAAAACAGAAAGAAAGAAAGGTAGTGTGGTATGGCATTGAAGAACTGACTGATGGACAAAC
AGAAAGAAAGTCCTGATGATAAAGAAGGAGCCAAAGAAAGAGGAACTGTACATGTATGGCAGGCTGGTACAAACCCAGTCAACCCAGAGCGACTTCAAGG
TTTGGGAGGAAAAATTGTGCTTCAAGCAAGCCTTGGGAAAAATCATGGACTACTGCTAACTAGAGACGGGGAAGTTTTTAGCTTTGGAAAGCAGCCATGG
AGGGAGGAAGAGACAACCTACGAAACTCTTGTTTTAGAGAAAACTCTATGTGGAGAGCAAGTTATCTGTGTAGGTGCAGGAAGCTACCACAATGGAGCCG
TAACAGAGAGTGGAATGGTTTACATGTGGGGGGAAAACACTGCAGGACAGTGTGCAATTGCAAACTACCCTTCCATACCAAAGCCTACAGCTATTACCAT
AACTGACCAGGAAACCACCCCTCCAAGGTGGGTAAGGACTATACAGCTGTCATGTGGAGAAGAACATACCCTTGCACTATCTGTCAGCAGAGAAATATGG
GCATGGGGCTCTGGCTGTCAGCTTGGATTGATAACCACCACTTTTCCAGTAATTAAGCCACAAAAGGTAGAGCATCTGTCAGGACGGATTGTTCTTCAGG
TTGTCTGTGGGGCATTTCACAGCCTGGCCCTTGTTCAGTGTCCCATTGCTCAGGACTTAAAGCCTGTTCCAGAGAGATGCAACCACTGCAGTCAACTGCT
CATTACAATGACTGATAAGGAAGACCATGTTATCATTTCTGATGCTCATTGCTGCCCACTTGGTGTTATGCTGGCAGAATCCCACCCTGGCAGCAGAAGC
AATCAATCTTCTTTTGGACTTAGTGTTAAAAGTGTGAAAAAGGAACATATATCACACTCTGCCATGACCCATGAAGAAAAGGAGACCTCCCAACAATATC
TAGAATCAGATACAGAGCTCGGCACAGATGCTGCTAGTTTTTGTGAAACTGAAACATCTCAGATTTTTGGCAAATCTAATGGAACAATCCCAGCTCTGTC
TCAAAGTGAGCAATATCTAAAGGATGGAATGGCAGAAGTTACACAGCTGCAAGAGCAAAATCCTTTAGAAAACGTGAGTGAGACTGCTGGGAGCTCCACT
GCTATCGCAACATCAGGCCTTAACAGTCTGATGGTATCTTGTGCCTCTGCTGTAAGTGTGAGGGTAGCCGCTACCTGTGAAGCCGGGGCTCTTTCACTCA
AGAAAGTCATGAATTTTTACTCTGGATCTCTATGTGACCCAGGAGTTCAGCAAGGTGTCAGTTCCTCTGCACAGGATGGACTGAAGGATAGTCGGGAAGA
ACAGGTTAAACTGGAGTCAATGCAAGGCAAGAAGAGTTCCAGCCTTGTAGACATCAGAGAAGAAGAAGCAGATGCTGGAAACAGAAGACTTTCTCTTCCA
GGCCTGCTTTCTCAAGTGTCTCCTAGACTTCTACGGAAGGTAGGACGAGCAAGAATGAGGACTGTGGCTCTGACACCCACAAACAGTGGTGAGGCTGATG
TGCTGCTGCCCTCTTTAAAAACTGAGGTGTGGAGCTGGGGTAGAGGAAAGGAAGGGCAACTGGGACATGGAGATGTCTTGCCTAGGTTGCAGCCACTCTG
CGTGCGAAGTATGGATGGCAAAGAAGTGATTCAGTTGGCAGCCGGCAGTCACCATTCATTGGCTCTTACTGCCAGATCTCAGGTTTATTCTTGGGGCAGC
AATTCCTGCGGCCAACTTGGTCATCTGCAGTCCCCAACCACAATGCCACGTCTTGTTAAGGTGTATGAAAGTGATGGTGTGTGGTCTGTAGCTGCAGGAC
GGGATCACTCCCTTTTTTTAGTACACAAAAAAGAAGAATTTCAACCTGCTGTGTATTACTGTGGACTTCAAACAGTAAACAACTGGAGTGGACCTGACCC
AAGCCAATTCCCAATAGAGCTCAATGAATGTGGCAAGCTTGGCTACATTTGTAGTATCTCTGCTGGAGCTGATTGCTGCTTAGCCCTGGTAGACCAAAAT
ATGATGGGTTATATTGGAAGTCTTCATGAACTTGCAGATACTGAAAGACGCTTTTACAACCATCTTAGTGATATGAAGTCTCTGATACTTAGACCTCTCC
TAGGGCTTGAAAACCTAGGTTCTGCTACTGCACTCCAGCAACTACAGAATATGGCTACTAAATTCAGCAAACTCTGCTATTTGATTGGCCAGCATTCTAC
CTCACTTGTTCACTTCCTACGTGGAGCAAAGGATGCTCGGAGCCTGTCTATTCTGGAGCATTCCAGTCTTTTCCTAGACAGCTATACAGAGTATTGTACT
TCTTTCTCCAATTTCCTAGTCATGGGAGGATTCACTGTTCTTTCCAAGCAGGCAATGGAATTCTTCAGCAAGAACCCGGAACATTTGCAGATTTTTTCTG
AGGGAACAGAGGAGAGCATCTCTATAGCAGAGAAGTTGAATTCTTTCTTTTTCTCTCCGATGAGTAGATTGCAAGAATATGCCAGGCTGCTTCTAAAACT
TGCAATGTGCTTTGAAGTGTCCTCTCCAGAATACAAGAAACTGCAAGATTCCAGTTCAAGCTATGACTCTCTGGCCTTGCTAGTGAACAGAAAGAAGAAG
GATGCAGAATACACACTAGGTTTCTGGAAGACCTTCCCTGGAAAGATGACGGACTCCCTGAGAAAGCCAGATAGGCGCCTAATTTGTGACAGTAGTAACC
GTGGCCTCTCCCTGCAGAACGCAGGACGTTTTTCTGTTAACTGGTTCATCCTGTTCAATGATGCTTTGGTGCATGCTCAGTTTTCCACACACCACATTTT
CCCCCTTTCTACAGTCTGGGTAGAGCCTTTGTCAGAAGAAGTTAATGGAGTTAACGGTCTGAAGATCACCATCCCAGAAGAGCAATTAACTCTAGTTTCT
TCATCTCAACAGGAAAAGGCTAAATGGCTTTGGGCAATTAGTCAGGCAGTGGATCAGGCCTTGAAGGGTATTACTGAGCCACTTGCCCAATCAGGTGGAA
GTGTACATCGGCAGGAACCCCCACTGTCACGCACTGCCAAGTATACTTTCTACAAAGATCTTCGGCTAAAAGATGCCAAGTATGAAGGCCGCTGGCTTTC
TGGAAAACCACATGGCAGGGGGACTCTGAGCTGGCCAGATGGAAGGATGTATTCTGGGACTTTTCGCAATGGCCTGGAGGATGGATATGGAGAATGCACA
ATCCCAAATAAAACTTTAAACAAGAATGATCGCTATGCGGGACACTGGAAAGATGGCAAAATGTGTGGCCATGGCACCTACTGGTATGCAATTGGAGATG
TCTATGAAGGGTGTTTTCAAGATAATATGAGGCATGGACATGGCCTTCTTAGGAGTGGAAAACTGACTTCCAATTCACCCAGCATATTCATTGGCGAATG
GGTAATGGACAAAAAGTGTGGCTATGGAGTTTTTGATGATATCACAAGGGGGCAGAAATTCATGGGAATGTGGCAAGATGATGCCCGGCAGGGTAACGGG
CTAGTAGTCACACAGTTTGGCCTTTACTACGAAGGTGCCTTTAACCTTAACAGGATGATGGGATGTGGGGTTTTACTATCTGAAGATGACACAGTTTACG
AGGGAGAGTTCTCTGATGACTGGACTCTGAATGGGAAGGGAGTTCTTACTATGTCGAATGGAGATTGTATTGAAGGGGTTTTCAGTGGAGAGTGGGGATC
AGGCATAAAAGTCAGCGGCATGTTCTCTAAGCCAAACTTATTTGATGGGGAAACAGAGAAGGAAAAAAAAAAAAAAA
5' EST: CX918885 [+]tgtcagacaggcaacaaggagctttcaattaacaggattgcgggacgtgttactggtagaacaacatctacactagagattttaaactataattatcttc
caaggaggtgtgctacaggaataaatcactgactgatgataaaacagaaagaaagaaaggtagtgtggtatggcattgaagaactgactgatggacaaac
agaaagaaagtcctgatgataaagaaggagccaaagaaagaggaactgtacatgtatggcaggctggtacaaacccagtcaacccagagcgacttcaagg
tttgggaggaaaaattgtgcttcaagcaagccttgggaaaaatcatggactactgctaactagagacggggaagtttttagctttggaaagcagccatgg
agggaggaagagacaacctacgaaactcttgttttagagaaaactctatgtggagagcaagttatctgtgtaggtgcaggaagctaccacaatggagccg
taacagagagtggaatggtttacatgtggggggaaaacactgcaggacagtgtgcaattgcaaactacccttccataccaaagcctacagctattaccat
aactgaccaggaaaccacccctccaaggtgggtaaggactatacagctgtcatgtggagaagaacatacccttgcactatctgtcagcagagaaatatgg
gcatggggctctggctgtcagcttggattgataaccaccacttttccagtaattaagccacaaaaggtagagcatctgtcaggacggattgttcttcagg
ttgtctgtggggcatttcacagcctggcccttgttcagtgtcccattgctcaggacttaaagcctg
3' EST: CX918884 [+]ctctgtttccccatcaaataagtttggcttagagaacatgccgctgacttttatgcctgatccccactctccactgaaaaccccttcaatacaatctcca
ttcgacatagtaagaactcccttcccattcagagtccagtcatcagagaactctccctcgtaaactgtgtcatcttcagatagtaaaaccccacatccca
tcatcctgttaaggttaaaggcaccttcgtagtaaaggccaaactgtgtgactactagcccgttaccctgccgggcatcatcttgccacattcccatgaa
tttctgcccccttgtgatatcatcaaaaactccatagccacactttttgtccattacccattcgccaatgaatatgctgggtgaattggaagtcagtttt
ccactcctaagaaggccatgtccatgcctcatattatcttgaaaacacccttcatagacatctccaattgcataccagtaggtgccatggccacacattt
tgccatctttccagtgtcccgcatagcgatcattcttgtttaaagttttatttgggattgtgcattctccatatccatcctccaggccattgcgaaaagt
cccagaatacatccttccatctggccagctcagagtccccctgccatgtggttttccagaaagccagcggccttcatacttggcatcttttagccgaaga
tctttgtagaaagtatacttggcagtgcgtgacagtgggggttcctgccgatgtacacttccacctgattgggcaagtggctcagtaataccccttcagg
cctgatccactgcctgactaattgc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: