XB-CLONE-3250616
Clone Name: IMAGE:7692604
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Gene:
nfix
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC166215 [+]
gcaggggagagagggaacgagagagagagagtttaagcggacagaaaaaaaaaacagctttcccttctgctccgaaccttctgaaaaaaaaaaccccatc
ttcctcttattccttttcgtttacctgttttcctttctctttttttttggttgaaaatattgtctgccaaattcccaaggatacgacgcgcccccccccc
cctattttctcgccatgccatttgtatccaagatgatattttttcctttttttcctgctgcggtcttgcgtccatgagttgttcttgatttgaggccact
ttatctttccctaactttgtgtgtgttttggggtgttgttttgatccccgtgtaaatatatacattttgggaagcgtttattggctgtcccacgtaaact
cacccttttcacccctacatatgctcaattgcttgtcttatccccccctgacagtctctcggtcatctatcaatcgctttctctctccataattctgctc
aacttggaatttaggtggttttgagtttctccggcagttcgctatgacaccaagagatggatgaatttcaccctttcatcgaagctcttctcccacatgt
ccgcgcattttcgtacacatggttcaacctccaagcaaggaaacgcaagtatttcaagaagcacgaaaagcggatgtcaaaggacgaggagagggcggtg
aaggatgagttgttgggagagaagcccgagatcaagcagaagtgggcgtctcgactgttggctaagcttcgtaaggacatccggccagagttccgagagg
actttgtccttactatcaccggcaagaagcctccatgttgtgtcctctctaacccagaccagaaagggaagatccggaggattgactgccttcgtcaggc
agacaaggtgtggaggttggacctggtgatggtcatcctgtttaaggggatcccgctggagagcaccgacggggaacgtctctacaagtcaccccaatgc
tctaacccagggttgtgtgtccagcctcatcacattggggtgtccatcaaagagttggacctctacctggccttcttcgtccacacacccgattctggac
agtcggacgcttcaaatcagactgaggtggatataaagccccctccaaatggtcacctgtcctttcaggattgctttgtcacctcaggagtgtggaacgt
cactgagcttgtacgcgtgtcccagacacctgttgccacatcatctggccccaacttctcattggctgacctggagagcccaagctattacaatataaac
caggtgtcacttggtaggcggtccttaacgtcgcctccttccagcagttctaccaagcgccccaagtctcttgatgacagtgagatggagagtccagtgg
atgatgttttttacccggggacaggtcggtcgcctgcagcaggaggcagccaggccagtgtgtggccaaatgatgtagacacagggcctgggtcacttaa
aaagccgggaaagctggatttctgcagtgctctgtcctcacagaccagttcctctcgaatggcattcacccatcaccctctcccagtgctggcaggagtg
agaccaggaagccctcgggcatcagcgtctgctcttcacttcccctcaacgtccatcatccagcaatccagtccttacttcacccatccaacaatacgct
atcatcaccatcatcatacccaggactctcttaaagagtttgttcagtttgtctgctcagacacctctggccaggggtcaggacagcattctcagcgcct
tcccccgccgggtccaacaggtttgtcacaattggatcacgagacaccaactttctgaacctcccccagcagtctcaatcctggttcctttgatattacc
gccaagctgcgaatatgcgagctaaaggaacaacgttttgtgacacagaagcccaccctttacacaccgaccccccccccctccaccccatgcgtgacta
caggacgacaacccctttctcccccccccccctcgcccagagcaagaacggaacagcgaaactctcacggagcgcgtgggatggaaattcccttactggg
gcgaactttttaatgtctgcgctgcctagcacccctctgatagttaaggtttaaaccactcatgtttcacccttccctcaccttccgccaacctatcatg
tattttcagtgtttgttccctttgtcattcacactcttttcccccccaggagaaaggattgagctatgggacattggtcccctatagggtgacagttaga
taagggacacgcagtgcttccctcccagttcagggagacagatcgaaacataaaaaaacgatacatttcctagttagtgccgctaatttgtgaccactgt
ttccgaatgattggaatctcaccggaatagaagccttccccccagtaacatttatacagccatttgtcctatcaaccttcccattcagcaaaacagaaat
aacgtcatcgatatatctccccttacgattgcgttaatccgccttcgcctgggcgtaaacacgcgggacgaggatagaacgttaaacgctcaagtgcaat
gatttcaattagcgtaggatgttttttttttttttttttcttaaggttaatatttaaacgagcaaaaatacatttctattttcgtgtaaaacgggcaaaa
aaaataaatttgctttctgtttaaaaataaaaagtccgcgtctcccagtggactctaaaaacacagggaagctgtctgccatcagacattaagaaattgt
tttttaaaaattattttcttgcactaaataagattttttttttccggcatgttttctccttccattgcactatgacttttattaacagatcatttgtata
aaaaaaaaaaaaaaa
5' EST: CX919212 [+]caggggagagagggaacgagagagagagagtttaagcggacagaaaaaaaaaacagctttcccttctgctccgaaccttctgaaaaaaaaaaccccatct
tcctcttattccttttcgtttacctgttttcctttctctttttttttggttgaaaatattgtctgccaaattcccaaggatacgacgcgccccccccccc
ctattttctcgccatgccatttgtatccaagatgatattttttcctttttttcctgctgcggtcttgcgtccatgagttgttcttgatttgaggccactt
tatctttccctaactttgtgtgtgttttggggtgttgttttgatccccgtgtaaatatatacattttgggaagcgtttattggctgtcccacgtaaactc
acccttttcacccctacatatgctcaattgcttgtcttatccccccctgacagtctctcggtcatctatcaatcgctttctctctccataattctgctca
acttggaatttaggtggttttgagtttctccggcagttcgctatgacaccaagagatggatgaatttcaccctttcatcgaagctcttctcccacatgtc
cgcgcattttcgtacacatggttcaacctccaagcaaggaaacgcaagtatttcaagaagcacgaaaagcggatgtcaaaggacgaggagagggcggtga
aggatgagttgttgggagagaagcccgagatcaagcagaagtgggcgtctcgactgttggct
3' EST: CX919211 [+]acaaatgatctgttaataaaagtcatagtgcaatggaaggagaaaacatgccggaaaaaaaaaatcttatttagtgcaagaaaataatttttaaaaaaca
atttcttaatgtctgatggcagacagcttccctgtgtttttagagtccactgggagacgcggactttttatttttaaacagaaagcaaatttattttttt
tgcccgttttacacgaaaatagaaatgtatttttgctcgtttaaatattaaccttaagaaaaaaaaaaaaaaaaaacatcctacgctaattgaaatcatt
gcacttgagcgtttaacgttctatcctcgtcccgcgtgtttacgcccaggcgaaggcggattaacgcaatcgtaaggggagatatatcgatgacgttatt
tctgttttgctgaatgggaaggttgataggacaaatggctgtataaatgttactggggggaaggcttctattccggtgagattccaatcattcggaaaca
gtggtcacaaattagcggcactaactaggaaatgtatcgtttttttatgtttcgatctgtctccctgaactgggagggaagcactgcgtgtcccttatct
aactgtcaccctataggggaccaatgtcccatagctcaatcctttctcctgggggggaaaagagtgtgaatgacaaagggaacaaacactgaaaatacat
gataggttggcggaaggtgagggaagggtgaaacatgag
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: