XB-CLONE-3250034
Clone Name: IMAGE:7693312
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Gene:
grn
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122897 [+]
GGCGGGAGTAATATTCCAAAGGGTTCGAGCGGCGTCCTGTTTTAATGGCCGGGGCCTATTCAGTTAGTGGCGCAATTTAGTAGCTGCTTGATGTATATTA
TGGGTGCATATGGCAGCTTTCTGCCTGGATTTGATATCCCAGCCCGGTGCCACCCCATCTATCAAAGTACCACAGCTCCTGTGTAGTTTATGTTCCCAGG
TGCTGAGCTATTTTTCTCAGTCATAGTTGTGTGAACACAAAAGGGTGGAAGCAATCTGGTATAAAAATATTTTTACACATTCTGAAACAAAAGTCTCAGC
AGACTCTGATTTTGGAGTGAGCGGGGTAAGTGGGCGGAGCGCAGCGCATAAACCGAGGTCAATTGCTGGGAAGCTGTCACATGCACAGAGCTGGGCAGGA
TGGGTCCACCTTGGTTTCTTCTGCTTGTGGTCTCCACTGTTAGTGCCACACTCTGCCCAGATGGCAGCACTTGTGGAGAAAAGAGTCTGTGCTGTGAACT
GCCTGGCAAGAAGGGGTATGGCTGTTGCCCTGCGGCAGAGGTTGTGTCTCGCTCCCTTCCTATGATTCTTTCCCAGACCTCCTGCTCAGGCTGCCCTGAT
GAGTATTCCTGTGTGAATACTCCAGAAGGGGGAACCGCTTGCTGTCCATTATCTGAGGGAAAATCTTGCCAAGATGGACATCACTGCTGTTCTGTAGGCT
CCTACTGCTCAGATGATGGCCACTACTGTATCCCAGCCTCTAACCAGTCTGCTGTAGTCTGCCCGGATGGAAGGTCTGAATGCCCCACTCTCACCACCTG
CTGTATGATGTCTGACATGTCGTCATGGGGGTGCTGCCCCATGCCACAGGCAGTTTGCTGTGATGATCATATGCACTGCTGTCCCCATAACTCTGAGTGT
GATGTCCAGCAAGGGCGCTGTATCTCCAACCAGGACCACATTCCCTGGATGAGCAAACTTCCAGCCCGTGTGAAATCAGAGGGCTTGAAATTAGTTGGCT
TGGGAGATGAAGAACGACGGGTTCCATGCCTTGATGGCACTTTCTGCCCAGATGGCTCTACCTGCTGTGAACAAGTAGACCACACATATGGGTGCTGCTC
CATATTGTCTGCTGTCTGCTGTTCTGACCATCTTCACTGTTGCCCTGGTGGTACCACATGTGACCTTGTCCATAAGAAATGTGTATCCCAAACTGGGGAG
GGCCCCTTATTGCCACAGATGCCAGCTATCCGGGAGGAATCAGCCAACCAGGTCCCCTGCGATGCCACCACTAGCTGCCCGGATAAGAACACCTGCTGCC
ACCTGTCATCTGAGAAGTGGGGTTGCTGCCCTTATGCACAGGCAGTGTGTTGTGACGATCATATCCACTGCTGTCCCAGTGGGTTCACATGTTCTGGAGG
CAGCTGTGTGTTGGCAGAGCACTCTATCCCTTGGATGAGGAAGACTTTGGCTCAGGGGCTGAAAACTACCAGAGTTCAGTGTGATGACACCGCCAGCTGC
CCAGAAAAGGAAACCTGCTGTCGTCTGGTATCTGGCAAGTGGGGTTGCTGTCCTGTAGTGAAGGCTGTGTGCTGTAATGATCATCTTCATTGCTGTCCTG
AAGGTTATACATGCTCCCAGGGCGAATGCTCAAAAATGGAACACTCCATCCCTTGGTTCACAAAAACTCCAGCTCTGACCCATGAAGCCAGAGATGTCGA
ATGTGATGATATGTACAGCTGCCCAGATGGACAAACCTGCTGCCGCTTGGCCTCAGGCGACTGGGGATGCTGCCCAATAGCACAGGCTGTGTGCTGTGAT
GACCATGAACACTGCTGCCCTCCTGGTTACACATGTTCTGGAGGAAGTTGCCAGAAGGGGGAGCTCTCTATCCCATGGTTCCTCAAGACTCCAGCTTTGA
AACAGAAGGCCAGAGATGTCCAGTGTGATGATATGTACAGCTGCGCAGATGGGCAAACCTGCTGCCGCCTGGCATCTGGGGACTGGGGATGCTGCCCTAT
AGCACAGGCTGTGTGCTGTGATGACCATGAACACTGCTGCCCTCCTGGATACACATGCTCTGGAGGAAGTTGCCAGCAGGGGGTGCTTTCAATCCCATGG
TTCCTCAAGACTCCAGCTTTGAAACAGAATGCCAGAGATGTCCAGTGTGATGATATGTATAGCTGCGCAGATGGGCAAACCTGCTGCCGCCTGGCATCTG
GGGACTGGGGATGCTGCCCTATTGCACAGGCTGTGTGCTGTGATGACCATGAGCACTGCTGCCCTCCTGGTTACACATGCTCCGGAGGACAGTGCCAGAA
GGGGGAGCACTCCATCCCATTGTTCAGCAAGACTCCAGCTTTGAAACAGAAGGCCAGAGATGTCCAGTGTGATGAAATTTACAGCTGCCCAGATGGGCAA
ACCTGCTGCCGCTTGGCCTCTGGCGACTGGGGATGCTGCCCAATAGCACGGGCTGTGTGCTGTGATGACCATGAACACTGCTGCCCTCATGGATACATAT
GCTCTGGAGGAAGTTGCCAGCAGGGGGAGCTCTCTATCCCATGGTTCCTCAAGACTCCAGCTTTGAAACAGAAGGCCAGAGATGTCCAGTGTGATGATAT
GTACAGTTGCCCAGATGGACAAACCTGCTGCCGCTTGGCCTCTGGTGACTGGGGATGCTGCCCAATAGCACAGGCTGTGTGCTGTGATGACCATGAACAC
TGCTGCCCTCCTGGTTACACCTGCTCTGGAGGACAGTGCCAGAAGGGGGAGCACTCCATCCCATTGTTCAGCAAGACTCCAGCTCTGAGACAGGAGACCA
AATATGTCAAATGTGATGATACCCACAGCTGTGCAGATGGGCAAACCTGCTGCCGCCTGGCATCTGGGGACTGGGGATGCTGCCCTATAGCACAGGCTGT
GTGCTGTGATGACCATGAACACTGCTGCCCCCCTGGATATACCTGCTCTGGGTCACAGTGCATGATTGGGGAGCACTCCATCCCATGGTTCAGCAAGACC
CCGGCTTTAAAACAGAAGGCCAAAGATGTCCAGTGTGATGATATGTACAGCTGCGCAGATGGGCAAACCTGCTGCCGCTTGGCATCTGGGGACTGGGGAT
GCTGCCCTATAGCACAGGCTGTGTGTTGTGATGACCATGAACACTGCTGCCCTCCTGGTTACACATGCTCTGGAGGACAATGCCAGAAGGGAGAGCACTC
CATCCCATGGTACAGCAAGACTCCAGCTCTGAAGCAAGAAGGTAACATAGTGAAATGTGATGATTCCTTCGCCTGTAAAGATGGTTACACTTGTTGTCGC
ATGGTCTCTGGGGTGTGGGGATGCTGCCCTATAGAGAAGGCTGTGTGCTGCTCCGACCACTGGCACTGCTGCCCCCAGGGGTTTACCTGTGATGCCAGAG
GTACCTGTGTACTAGGCCAGTTCTCTATCCCATGGCTCACTAAGGTTCCAGCTTTGCCCTTTGATGGGACTCACAGTATTTGTGACGATACCCACACTTG
CCCTTCTGGTACCACATGCTGCCCTGGAGAGGGGGGAGGCTGGAGATGCTGCCCAGTGGAAGAGAAGGAGGCTATCGAAACCTCCCCCAGTGACACCTCT
GTCCAGGGATTACGTCTGGATTATGTCTGGTGTGATTCTCAGTATGCCTGCTTTGATGGCCAAACTTGTTGTCGAGGACTCGGAGGAGTGTGGAACTGCT
GTGTTTATACTCAGGGGGTGTGCTGCCCAGACATGGTGCACTGCTGTCCCTATGGGTATGTGTGTCTGAACCATGGAACGTCATGTAGTCGTTCAGGAAG
CCCACGTTGGGATGGTCAGTCGGGAAGCCCACATTGGGATGGAAAACAGTCCCCACGGGATGGTAAACGGCCCCCTTTCCTATGAACACAATCCAAATCC
ACTTGATTGTTACTACTAATAAGATCCTTCTAAAAGCACTGGAATAAAATCAAATTATTTTTGTTTTATATGTTTAAAAAAAAAAAAAAA
5' EST: CX920201 [+]gcgggagtaatattccaaagggttcgagcggcgtcctgttttaatggccggggcctattcagttagtggcgcaatttagtagctgcttgatgtatattat
gggtgcatatggcagctttctgcctggatttgatatcccagcccggtgccaccccatctatcaaagtaccacagctcctgtgtagtttatgttcccaggt
gctgagctatttttctcagtcatagttgtgtgaacacaaaagggtggaagcaatctggtataaaaatatttttacacattctgaaacaaaagtctcagca
gactctgattttggagtgagcggggtaagtgggcggagcgcagcgcataaaccgaggtcaattgctgggaagctgtcacatgcacagagctgggcaggat
gggtccaccttggtttcttctgcttgtggtctccactgttagtgccacactctgcccagatggcagcacttgtggagaaaagagtctgtgctgtgaactg
cctggcaagaaggggtatggctgttgccctgcggcagaggttgtgtctcgctcccttcctatgattctttcccagacctcctgctcaggctgccctgatg
agtattcctgtgtgaatactccagaagggggaaccgcttgctgtccattatctgagggaaaatcttgccaagatggacatcactgctgttctgtaggctc
ctactgctcagatgatggccactactgtatcccagcctctaaccagtctgctgtagtctgcccggatggaaggtctgaatgccccactctc
3' EST: CX920200 [+]tttttttttttttttaaacatataaaacaaaaataatttgattttattccagtgcttttagaaggatcttattagtagtaacaatcaagtggatttggat
tgtgttcataggaaagggggccgtttaccatcccgtggggactgttttccatcccaatgtgggcttcccgactgaccatcccaacgtgggcttcctgaac
gactacatgacgttccatggttcagacacacatacccatagggacagcagtgcaccatgtctgggcagcacaccccctgagtataaacacagcagttcca
cactcctccgagtcctcgacaacaagtttggccatcaaagcaggcatactgagaatcacaccagacataatccagacgtaatccctggacagaggtgtca
ctgggggaggtttcgatagcctccttctcttccactgggcagcatctccagcctcccccctctccagggcagcatgtggtaccagaagggcaagtgtggg
tatcgtcacaaatactgtgagtcccatcaaagggcaaagctggaaccttagtgagccatgggatagagaactggcctagtacacaggtacctctggcatc
acaggtaaacccctgggggcagcagtgccagtggtcggagcagcacacagccttctctatagggcagcatccccacaccccagagaccatgcgacaacaa
gtgtaaccatctttacaggcgaaggaatcatcacatttcactatgttaccttcttgcttcagagctggagtcttgctgtaccatgggatggagtgctctc
ccttctggcat
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: