XB-CLONE-3249919
Clone Name: IMAGE:7693391
|
Gene:
kansl3
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX920426 [+]
gtgcggagcgttttagcagcgatccgggctgcgggttgtgtgaagcagcactgttgggcgataaagcaaatctgagcctctgggccgtgtccttgtggcc
tgggcagtatgtctcatagagggagtgagcgagacctgcagtcttctgctcgaaggatgggtacatctctgcttttccaactgtcagtgcatgaaaggga
actagatctggtatttttggatcacagctatgccaaaccatggagtgcacaccctgatgccagcaatgcacggccaacccgcaccctcttcattactcct
aggagacagcaagaaagtgtggttgaaaccgatgtccctattgatgttgaatctgtgacacccacacctatgccactgtatgacaatcacaaggcaagaa
atgttatgaatgagtgtgagcgtcatgtgatttttgtgcgaactgacacagaagctccacctccacctgatgactgggaagaacacctcaacaggtcagg
atggactgctgcacagaataaactttttaacaagattctaaaagccctgcagtctgaccgcctagcacggcttgctaatgaaggggctatcaacgagcct
gtgctccgtcggattgcagtggataaatgtgcacgtcgtgtacgccaagctttggcatctgtcagctgggacgcanagttggtgcagtggttgcatacca
cactagtagagacactcaacctgcccctgttagctgcatacctggatgcactgcagactctgaagggaaagatcccatctctcattgaccgcatgttgct
ggcgtccagtctgaaggcaggtggtgc
3' EST: CX920425 [+]tttttttttttttttaacatctagaaactgtttatatttacattttgtgacatatttacagttttcataactacacttttttttgaataggtttagcaat
tatatttacatttttggctattagattcagttctcatgccatgggagtagcttcaacaaaggccaaactcctcgccaccatagagattctgttctataaa
ctagaggttaaatagtgcatggcgctataaccaatttctgtggttcatagaagcatacagattactaaaatgtgaataagtcagatattccagtgtcact
gctgcttctgattggtcagtgtcccagagttttggataatgctgctggtgccagacagtgctattagagtaggctgaagaaggggtctgccgattagcga
ctcccggctgtgaattatggtgcttccatcatcaaccacggacggtaccctgcccagactctcaactgcgttcattacgtggtttcctgggatcttctct
gccgtttctgcctttgccaagatcacagtttttgggggagtctgaagaactgggcctattttggacagaaccacagtgcaggccggactgggagagtttg
caagggatggagtggtaaaagtcactgcaccagcagttacccctgaagtcgcctgcagagtcattgacttcagtgggagagaggccagatcgctgcccac
agacttggtaagagcagaaagcttggcatccgacataacatagagagtcttgggtggactggttgttactgccggaggggtcactgccctggctaagggc
aaggatttttc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: