XB-CLONE-3249487
Clone Name: IMAGE:7693927
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Gene:
klf7
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122980 [+]
GGCAATTCATGTCAGTGAGTTTGCATCTTTCTTCCTGGTACAAGCTTTGGAGTGACTACAGCCTTGCAAAGGATTTCTTGCATCCCATAACCACATTGGA
TGTTGACTTTTTACAACTGTTTCATTTTTTTTCTATTTCTATCACAATTTCTCTTAAGCAACCTCCTTCTTTTTCACATGTTTGGGTTTGAGTGATCAGC
ACTTATATGCTTTTTTTCCTGCTCTCTGTAATCTCCACTGACTGTAACAACCAACACAAAACATTTTAAAAAAAAGGGGACTGTGGATCTATTTAAGGAG
GACATCGCTCACGGAATATACATATTTTTTCTTGCATAGCAAACATGGATGTTTTGGCTAGTTATAGTATATTCCAGGAGCTCCAGATTGTACATGACAC
GGGCTACTTCTCAGCAATGCCATCCCTGGAGGAAAATTGGCAGCAGACTTGCCTGGAACTGGAGCGGTATTTGCAGACAGAACCTAAAAAGATGTCAGAA
ACCTTTGGAGAAGATTTGGACTGTTTTCTGAACACTTCCATTGCTCAGGCTGTTGATGGTACTGCCCGTAAGATTGATTCTATTGTAATTCCAGTGGATA
TTAGTGCATATGAAAAACGTCCAAGCATGGATGTTTTACTTTCACAAGACAAGCTACTCTCAGAGACGTGCGTCAGCCTACAGCCTGCTAGCTCTGCTAC
AGAATGCTACACGTCTGTAAACCAAGCGCAGCTGAATGCCGTGACGTCTCTAACACCGCCATCATCCCCAGAACTTAGCAGGCACCTTGTCAAAACACCA
CAGTCCCTTTCTGCTGTAGATGGAACCGTGACCTTAAAACTAGTTGCAAAAAAGGCCTCGGTTAGCTTGGGGAAGGCTGCAGAGTCAGCATCAACAGCTG
TCACGTCAAAGTGTGGCCTGAGCGATAGTGAACAGACTGGGGGCCCAGGAGAAGCTTCCCCTGAAAATAAGAAAAGGGTCCATCGCTGCCAGTTTAATGG
CTGCAGGAAAGTCTACACAAAGAGTTCACATTTAAAAGCCCACCAGAGGACACACACAGGTGAGAAGCCATACAAGTGCTCCTGGGAAGGGTGTGAGTGG
CGTTTTGCTCGAAGTGATGAGCTAACAAGGCACTACAGGAAGCACACAGGGGCAAAGCCATTCAAATGCAACCATTGCGACAGGTGTTTTTCAAGGTCTG
ATCACCTTGCCCTTCACATGAAGAGACATATTTAAAGACATTCATAATAAAGATGGCATCCCAGCCTTGAACAGACCATGGATTGCAGTTGGCACTCATG
GTTATGCAGGAGAACACTGTCACATGTAAAACTCTGTACACAAAATCCAAATCAAATGTTCATGCACCGTCACATTCATGCATATAACATCTGTTTGTTC
TGCCTTACCCCCAGCCTAAGTGAGAAAGGCATGGAAGGATGGAGATTTGAATGATTGAGGATCTACAAAATCCTAAGCAGTTGTTTTTTGGGTGCTCTTA
TATGTCTCACCAAGGAAATCAAGAGAACAAGACATCTTTGCAAGCGAACAGTAGATTTGGGAGGCTCTGAAGAACTCAATTTATTGCATCTCAGAAGCTG
CTAAAATCCACTTTCGGGTTGCTTGTTGTAGCACTAAATATTTTGTTTTAAAACTTTATGAGGTTAAAAATGATGGATTTCGACTAGGAAACCAAATCTC
AGTCCATGAGCCTTCTGCTTCATGTGCGTGAATCTGAAGACTATTAAAAAAATGTCCTATTTCTATTAGGATCCGGCTACATGGTCCTCTGTAGTTTTAC
CTGAATATGGAGCTTTTAAACCCCTTTTTAACTCAATGCCATAGGCCTTACTGGCAGTAAACAGGTGAAGTTCCAAGCATGACCAAGGTTCTGCAATAGT
TTGGTCTAAATTTATACAGAACTCTTGAATGGTAAAACATTTATATCCCCTCATTCCCTTAATTTATTTATTTTGTACTTATTTCGGCTGTGGCACACCT
TATGCACTAGTGACTTTTAAAGCCGCTAACACTGACTGAGTGGGATCGAAATGATTAAAACCAGAGTGTCCTAACTACCAGTGTGCGCACTTCCAAAGGT
TACAGACCTTACCCTTTTTGTTTTGTCACAGTAACCTTTTTACTTGTGTTTAATTTAAACAGGCTCAATGAGAAACATTGTTCATTTACAGTACACTCTC
TCGTTGGACATTTATCTTATTAAACTTCTTGCTCTCTGTCTGGACACTTTTAGCTTGGATTGGACCAGCAATTTAGACTTGACTTCTCATGTCAATGGGC
TGTAAGCCGTCTCCAACTGAACTTTCCAAAATTGTGCTTGATTAGTGATTTTTAATCCTAACAATATAACTAGTTAGAAACTTGATGTTATTTCGAGAGC
AGCTGCATTTTTGTACTAAATATTTTGTTTCAGTGTCTAACACCAACAAAAAGCCAAATATATAAATCCATACAACACTGGATATTGGAGGACACCTTAA
AGTACAGTAAGTTGACTTTGCGGGATTGACTCATTAATGATCGACTACCTTGAATACCAAGGTCATTATTAGGATGCTCTTAAATTAGTTAAGAGCTCAG
ATATCTGTAAACTTAGTGGATATCATTTCCATTGGTGATTTTTTTACAGGGTTACAGTAAAATAGCTGTAAGTTCATTGTGATCTCATGTTAGTAATATC
TTAGGGTCACAAAACAACTTTACGTACCTGTCAAAATCAGTAATTTTCTGATATTTTTAAAATGGCCCGAAATCCATCACAATCAGGAAATTCATGTACA
TACTACTATGCTGTCGCTAGTCTCTAGAGACTGCCAATCGTATCATATCAAGTGGTCAATTCAAGTAGTTGTGGAGGGGGATAGGTTGTCTATTTTGTAT
ATTTTGGGACCATCCCTAAACAACTAGTAAAATTGCACATTTGGTCCCACTGTGTCCCTGATGAACGCAGCTCTTTTATCCCAGCCAACTAGCATATTTT
TAACTTGTTTTGACTCCTTGACTGTTAAACAGAAAAAAAAAAAAAAA
5' EST: CX921194 [+]gcaattcatgtcagtgagtttgcatctttcttcctggtacaagctttggagtgactacagccttgcaaaggatttcttgcatcccataaccacattggat
gttgactttttacaactgtttcattttttttctatttctatcacaatttctcttaagcaacctccttctttttcacatgtttgggtttgagtgatcagca
cttatatgctttttttcctgctctctgtaatctccactgactgtaacaaccaacacaaaacattttaaaaaaaaggggactgtggatctatttaaggagg
acatcgctcacggaatatacatattttttcttgcatagcaaacatggatgttttggctagttatagtatattccaggagctccagattgtacatgacacg
ggctacttctcagcaatgccatccctggaggaaaattggcagcagacttgcctggaactggagcggtatttgcagacagaacctaaaaagatgtcagaaa
cctttggagaagatttggactgttttctgaacacttccattgctcaggctgttgatggtactgcccgtaagattgattctattgtaattccagtggatat
tagtgcatatgaaaaacgtccaagcatggatgttttactttcacaagacaagctactctcagagacgtgcgtcagcctacagcctgctagctctgctaca
gaatgctacacgtctgtaaaccaagcgcagctgaatgccgtgacgtctctaacaccgccatcat
3' EST: CX921193 [+]aacagtcaaggagtcaaaacaagttaaaaatatgctagttggctgggataaaagagctgcgttcatcagggacacagtgggaccaaatgtgcaattttac
tagttgtttagggatggtcccaaaatatacaaaatagacaacctatccccctccacaactacttgaattgaccacttgatatgatacgattggcagtctc
tagagactagcgacagcatagtagtatgtacatgaatttcctgattgtgatggatttcgggccattttaaaaatatcagaaaattactgattttgacagg
tacgtaaagttgttttgtgaccctaagatattactaacatgagatcacaatgaacttacagctattttactgtaaccctgtaaaaaaatcaccaatggaa
atgatatccactaagtttacagatatctgagctcttaactaatttaagagcatcctaataatgaccttggtattcaaggtagtcgatcattaatgagtca
atcccgcaaagtcaacttactgtactttaaggtgtcctccaatatccagtgttgtatggatttatatatttggctttttgttggtgttagacactgaaac
aaaatatttagtacaaaaatgcagctgctctcgaaataacatcaagtttctaactagttatattgttaggattaaaaatcactaatcaagcacaattttg
gaaagttcagttggagacggcttacagcccattgacatgagaagtcaagtctaaattgct
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: