XB-CLONE-3249373
Clone Name: IMAGE:7693956
|
Gene:
sec23ip
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC166374 [+]
gtggaacttgtgggacggacatggcggggaaggtacctaacggggctcagtccgggccgcctggcaacaacctgctcttctccgcctccgagatcagctt
ctcccttcccttcatccctgtgagccagcacggccccgcaatgcttccgagcgatgatgccgctgaggtgggagaagaggacagctttctaggacagact
tcggctactactaacccaccacaaacctttagttatttctcccagggaccctccctaggcagcagccaagatccatttgcgtgtatttggcatccacctc
taaccacagaggcgcctcagcccgttacttcggttttcttcaagcccccccccgctcccacccctgaggccggacacagtatgatcccatcgaaggcacc
aaaacccccggttacaagcaacactccagtatcccaggttacagctggtgtctttcttccaacgtcacagcacggacctcttcctggcacttactcggct
ccgcccactgcggcctctcagggggggtataacccctaccgccacaatacccagagcagccgggtgaatccatactttgctcccccacagcttctgcagg
accagggtttcgctccctttatccagactcctccattgccacctctgccccatcagggggcgccaacctctctcccaccgccccctaaaactgcacccca
catgcctccccctccgccatcacagacctttccaaatgtcagacctgccggaccatcagtaccagtacccccaccctacctgccaaacctgtatgaaccc
gttcagcctcactggttctattgcaagcaagtggagagcaaggaggtctggctgccgttcagcgctctggattcccacggtttagaagaggtttataact
ccgtgcagcctgaccccgagagcgtggttgtgagcacagacgggggacgctatgacgtgtgcctgtacaatcgggagaagagggccgtattttgggagga
ggaggactcggaagtcaggcgctgctcttggttttacaaaggggacgcagacagcaagtttgttccttacagcgaggacttcagcgagaggctggaggca
gaatacaaaaaagctgtgaccaccaaccagtggcatcggagactggaatttcctggaggggaaacaatcgttatgcacaaccccaaagtgatcgtccagt
tccagcagtctcttattccagatgaatggggctccccacaagacgggcagtcacggccccgagtcgtaaagcgaggcattgatgctgaacttgatgaaat
tccagatggggaggtgccacaggctgatcatctggtctttatggttcatggcatcgggccagtgtgcgacttgcgcttcaggagtatagtggagtgcgtg
gacgacttcaggacagtgtccctcaaactgctgcagactcactatagaaagtccctggaagagggcagaatgcgtcgggtggagtttctcccagtccatt
ggcacagctctctccatggagatgccacaggggttgataggcggataaagaagatcacattgcccagcattggccgcctgcgccactttaccaacgagac
cctgctggatatactcttctataacagccccacctactgccagacgatagtggacaaagtcaacatggagatgaaccgcctctttgcgctgttcatgagc
cgcaacccagatttcactgggggagtgtctgtggccggtcacagtttagggtccttggttctgttcgacatactttccaaccagactgatccaaccgctg
cgcctcttgtgccagtcccgccccctgaagccaacgggccaataaaaagtaaagcttcggatgagaaagcagtcacgctacaagcagtggaggagcccag
tgattccgagcaacagaaaccgctgccgactcttcgagaagcactggaatcgctcagtctggccgaatacagcaacgtgtttgagaaagagcagattgac
atggagtccctgctcatgtgtacggtggatgacctgaaagagatgggagtccctctgggcccaaggaagaagatcgccaagtttgtgcaggaaagagcag
ttactgaggcaaagagaaaagctgcagaggaagagagagctgcgcaaatcaaagccaaagaagcgttggctgctgccgcacaggaagccgctgccaaact
cccctcctctgaggatggcgccgccccggagtccaacaagaggaagttgcccgttggggtatccgtctcctctgtgccagtggattatgaatactttgaa
acgggcacaggacaggtctgtgtaaagtacaactcgctggactttgagcctgtcaatttcttcgccctcggctcccccattggcatgttcctgaccgtac
ggggcttggataagatcgaggaaaccttccggctgcctacgtgcaaaggcttcttcaatatctaccatccgctggatcctgtggcctacaggttggaacc
catgatcctcccagacattgacttgaagccggtgcttattcctcaccacaaaggcagaaagagattgcacctggagctgaaggagagcctgacccgtatg
ggctcggacctgaagcacgggttcattagttccctacgcagcgcctggcagaccctcaacgacttcgctcgcgcccacacgtcctcggcacaactgcaac
tggagctaaagaaggtcgccgatcagattgaggaagaggagaaacagcaggaagtggatgctgataaggtggagagcccagagacttcccgggaagagga
cgtcacagttaagatcgggatgctgaatggagggcgccgcattgactacgttctgcaagagaagcccatcgagagctttaatgaatacctgtttgctctg
cagagccacctgtgttattgggagtcggaggacacggctctgctgattctgaaggaaatctacacctccctgaatgtgctccctgaccagccgctgcact
agtgcgtccccttgagcgtgctctcctgctcataccaggtccctggatcccagagtggaaccggtacccaaaggacatttattcagactcttaggaagcc
cagtgcccctttgcctacgaggacgagccgctggcgccaaactgccacaatgtgaaaagtagtttttatatgaagctcgtctgtgcgaccccgagagaca
gcgagtggattttatcagtgctgtgacgctgcttgccaagtgtaacccgttatttggaacagaatggactctctttgctgaggactgaaccggatcagcg
cgtgtgtagggaaccggcagctctgtgctgcagccttttagttcttcatttaggtgccgacgatttgcttcccctttaactcattatgcccttacagttg
gtaatgccctttcctcttacagagagccccttccgccagtgagcaatgcgtttacaaagggagaatgagattttatcttgttttagcttgggccgatgcc
tcacaagcgacttttactcatagagagctaagggataggtttcatggttgattatggggcggggcacacagggtacagaggccggggtgcccccataaca
aacattactcccctataacagagatgtgtgtgcccgacccggcaatgcacctctatttatacacccgctcccgacccgcccccttcatgatgtcatgaag
ggggcgggccaatcatctatagaggcttctagacatggaggtcggacagtgcaaggtcacgggcggggagggcggaagaagctcagtcccaaccagcggg
tcccataggtttcaggccgccccgcgcatctctaccctacaagtctcctgtgcggctttggccccggccctggttttacattcccttctgcggataaaga
gagagagttgaacttggtgttttcagtaatttaatttttggaagaattcagttaaattaaaaacaaaaattgccaaaaaaaaaaaaaaaa
5' EST: CX921409 [+]tggaacttgtgggacggacatggcggggaaggtacctaacggggctcagtccgggccgcctggcaacaacctgctcttctccgcctccgagatcagcttc
tcccttcccttcatccctgtgagccagcacggccccgcaatgcttccgagcgatgatgccgctgaggtgggagaagaggacagctttctaggacagactt
cggctactactaacccaccacaaacctttagttatttctcccagggaccctccctaggcagcagccaagatccatttgcgtgtatttggcatccacctct
aaccacagaggcgcctcagcccgttacttcggttttcttcaagcccccccccgctcccacccctgaggccggacacagtatgatcccatcgaaggcacca
aaacccccggttacaagcaacactccagtatcccaggttacagctggtgtctttcttccaacgtcacagcacggacctcttcctggcacttactcggctc
cgcccactgcggcctctcagggggggtataacccctaccgccacaatacccagagcagccgggtgaatccatactttgctcccccacagcttctgcagga
ccagggtttcgctccctttatccagactcctccattgccacctctgccccatcagggggcgccaacctctctcccaccgccccctaaaactgcaccccac
atgcctccccctccgccatcacagacctttccaaatgtcagacctgccggaccatcagtaccagtacccccacctacctgccaaactgtatgaaccgttc
ag
3' EST: CX921408 [+]ttttttttttttttttggcaatttttgtttttaatttaactgaattcttccaaaaattaaattactgaaaacaccaagttcaactctctctctttatccg
cagaagggaatgtaaaaccagggccggggccaaagccgcacaggagacttgtagggtagagatgcgcggggcggcctgaaacctatgggacccgctggtt
gggactgagcttcttccgccctccccgcccgtgaccttgcactgtccgacctccatgtctagaagcctctatagatgattggcccgcccccttcatgaca
tcatgaagggggcgggtcgggagcgggtgtataaatagaggtgcattgccgggtcgggcacacacatctctgttataggggagtaatgtttgttatgggg
gcaccccggcctctgtaccctgtgtgccccgccccataatcaaccatgaaacctatcccttagctctctatgagtaaaagtcgcttgtgaggcatcggcc
caagctaaaacaagataaaatctcattctccctttgtaaacgcattgctcactggcggaaggggctctctgtaagaggaaagggcattaccaactgtaag
ggcataatgagttaaaggggaagcaaatcgtcggcacctaaatgaagaactaaaaggctgcagcacagagctgccggttccctacacacgcgctgatccg
gttcagtcctcagcaaagagagtccattctngtccaaataacggggtacacttggcaagcagcgtcacagcactgataaaatccactcgctgtctctcgg
g
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: