XB-CLONE-3249085
Clone Name: IMAGE:7694407
|
Gene:
mfn1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC166148 [+]
gattgtgtgtaggacaggaggcaaggaatttgcacttagctgctgtgcgggaggtataagtcatggaagaagcaagtgtctctcccctaaagcactttgt
gctagccaagaagagcattacgtctatctttgaccagttggtggagtatgtcagtgaaggcgtaacctttgtagaagaaacattccagaacccagagcta
aagaatgtgagtacagaggatgatgttaataaggttaaggcatctaagactaaactagctggcattggggaggttctcgcccgcaggaatatgaaggtgg
ctttttttggcagaacaagtagtggcaagagcacagtgataaactctatgctgtgggacaaagtgctgcccagtggtattggacacactaccaactgctt
ccttagcgtggaaggtacagaaggagacaaggcctatctgatgacagagggttcagaggaaaagaaaagtgtaaagactgttaatcagcttgcacatgct
ctgcacatggacaaagacttgggagctggctgtcttgttcatgtgttctggccaaaagcaaaatgtgctctgcttcgagatgacttagtccttgtagaca
gtccaggaacagatgtgacaacagagctagacagctggattgataaattctgcttggacgccgatgtctttgttctggtagcaaattcagagtctactct
catgaacacggagaaacattttttctataaggtcaatgagaaactttctaagcctaatatattcatccttaataatcgctgggatgcctctgcgtctgaa
ccagagtatatggaagatgtacgtaaacaacacatggaacgttgccagtcgtttcttgtggatgaactgaaagtagtggattccttagaggcacaaaagc
gcatcttttttgtttcagctaaggaagtcctgaatgccagaatgcacaaggcacaagggatgccagaagccggtgctgcgcttgctgaagggtttcacac
cagattcttggaattccagaaatttgaaaagctatttgaggaatgtatctcgcagtcagcagtgaaaacaaagtttgagcagcacactatcacagctaaa
caaattactgataccgtcaaagctattatggatgctataaacatcaaggctgcagacaaaagggtacggtgtatggtagaaagagagaatcagaatgaca
ggcttgagtatgtgaagacacaaatgctgatcctaacggaggaaattgagaaaaggattctgcatatcacaaaggaagtgaatgaacaggtctctagtgc
catgatggatgagatatatcatctttcccttctggttgacaagtttgacgctgagttccacccatctctgtccgttctgaagctttacaagaacgaactg
aatttgcacatagaaaatggcatgggcagaaaactggccgatcgctgttccaatgcagtgaacatgtccatccagaaagcacaagaagacatcatagaca
agctgatgcctttactgccgccctctgtccaagatcagactactatatctctgccatgtaaaaagtttgaccttagctacaacatcaactgtgaaaagct
gtgcagtgatttccaggaagacgttgagtttcgcttttcttttggtttgacgtcacttgctaatcggttccttggctgtaaaaccagccaccttttatta
ggctttgcagaaccaatgtttcagattccccggcctgtaagtaccccttcagcaacagggaatccagttttaccccctcaacctgcatctcaagaggaac
tcatgctttctgtcatgtccggattggcatctctaacatcaaggggttcaatgagtgtcatcatagttgggggagtgatctggaggactgttggttggag
gttgatctctggaatgatgttggcctatggtggtttatacctgtatgaacgtctgacatggacaacaagagcaaaagagagagcctttaaaaagcagttt
gtgaattatgctgctcaaaaactgaagttgatcgtcagcttcaccagtgccaactgcagtcagcaagttcagaaggagctggacaacacttttaaacgat
tatgtcagcaagtagatgtaacggaaagagacctagcagagtcaatagacaaactgtctgaagaaatacatcagctagagcaaatccagagtaactccaa
agcactcagaaacagagcatcacgaattgaaggagaactgagaaattttgcaaagagctttctacctcagaataagtaatcctgtggatctttgtacaga
tgatggtatccattcagcagaaactgcctccccaaagagtggatattgtacagacttggacactttgctgtcattaagtgttaatgcaaaagaggaattt
atatatttatattttggcaaataaaaatgtatttttgtgtataggcaacagacgaacagctctgttttaagcaaaaaatgaaaatttatagaagtgaaac
ctttatactataaaagtatattactggcattgtattatgttctgtgccactgcttgggaagcccttcagcttatcaatgtgtgttaggttctgacagtgc
cagaggctgctatattgtacaggtagggtgctgctggcagaatgggaaatggcataatgttcaactacagacctattcatctttgcctgttaggagctac
aggctggagagcactattataactggggtcctgccccgcaccttggttttgcaaccatttgtgttttgagtgttaggagttacttttcattacctcttct
gagacattacactacattagtttctccttgctaaatgctgtggttttatttcgcatgataatctagcagattaaaattctacctctctcctttggagaag
gccctgactgctattagtggatactggcatatgatagctctcctgcagtcctgtctatggaacaagccacatgcgcatgtttttaactgcaaataaatga
ggaagcccaggtatgtgcaggaacacttagcgcctctccatccttgctgcctatccctctccctgttgcccatctcttcagctgctttgggtaagtgcct
ggtcttcattcaaagtgaaaaaaataaatccaatgggaaaagtatttcttttgcaaagttctattactctggttggactttgtacgggcatagttgaggt
gaataccataatgggaattaatagcagtcaatgccacacactgacacctcactaaccaatcgcctgctgtcatgtggtacaatgaaggaaacaaacgggc
ccaataaccagtatctgttgctggctgtccaataggaatgaaggatcagtagggaaaaaaaaaaaaaaaaatatatatatatatatatatatatacatac
atacatacatacacacacacacatatatttagtggattgctgagaagtgtagtgtgatatgaatccaatgcagtcagggacatgattggcatatattccc
tgacagctgttttagttatttcatgaatttctgcaaaatcccctctccagcacagaaccactaatattcttaagagcatgcaatttaaagcaatgagaaa
atgcagttccagcctgtcctctgcatatttaatgactggcatgcaagactcaggttattgatatttatatggcgctgacctattcgcctatttatgagtt
cttacataaacgatttctatttgtaataataaatctgtgagcatggtttggaaatggttaaagctcttttatatattttattggaatatgatcattttta
aacagcatttataaataataaaagtctgattcactgtttaaaaaaaaaaaaaaa
5' EST: CX921932 [+]ttgtgtgtaggacaggaggcaaggaatttgcacttagctgctgtgcgggaggtataagtcatggaagaagcaagtgtctctcccctaaagcactttgtgc
tagccaagaagagcattacgtctatctttgaccagttggtggagtatgtcagtgaaggcgtaacctttgtagaagaaacattccagaacccagagctaaa
gaatgtgagtacagaggatgatgttaataaggttaaggcatctaagactaaactagctggcattggggaggttctcgcccgcaggaatatgaaggtggct
ttttttggcagaacaagtagtggcaagagcacagtgataaactctatgctgtgggacaaagtgctgcccagtggtattggacacactaccaactgcttcc
ttagcgtggaaggtacagaaggagacaaggcctatctgatgacagagggttcagaggaaaagaaaagtgtaaagactgttaatcagcttgcacatgctct
gcacatggacaaagacttgggagctggctgtcttgttcatgtgttctggccaaaagcaaaatgtgctctgcttcgagatgacttagtccttgtagacagt
ccaggaacagatgtgacaacagagctagacagctggattgataaattctgcttggacgccgatgtctttgttctggtagcaaattcagagtctactctca
tgaacacggagaaacattttttctataaggtcaatgagaaactttctaagcctaatatattcatccttaataatcgctgggatgcctctgcgtctgaacc
agagtataatggagatgtacgt
3' EST: CX921931 [+]tttttttttttttttaacagtgaatcagacttttattatttataaatgctgtttaaaaatgatcatattccaataaaatatataaaagagctttaaccat
ttccaaaccatgctcacagatttattattacaaatagaaatcgtttatgtaagaactcataaataggcgaataggtcagcgccatataaatatcaataac
ctgagtcttgcatgccagtcattaaatatgcagaggacaggctggaactgcattttctcattgctttaaattgcatgctcttaagaatattagtggttct
gtgctggagaggggattttgcagaaattcatgaaataactaaaacagctgtcagggaatatatgccaatcatgtccctgactgcattggattcatatcac
actacacttctcagcaatccactaaatatatgtgtgtgtgtgtatgtatgtatgtatgtatatatatatatatatatatatatttttttttttttttttc
cctactgatccttcattcctattggacagccagcaacagatactggttattgggcccgtttgtttccttcattgtaccacatgacagcaggcgattggtt
agtgaggtgtcagtgtgtggcattgactgctattaattcccattatggtattcacctcaactatgcccgtacaaagtccaaccagagtaatagaactttg
c
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: