XB-CLONE-3248738
Clone Name: IMAGE:7694495
|
Gene:
trim28
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX922570
[+]
acaaaggatcacaccatcaaagttaaaagttcttccaaaaaggaaacagagcgagcagtttactgtcctttgcataaaaatgaacctctcatgctttatt
gtgaatcctgtgataccttaacttgccgggactgccaactacagggacacaaagatcaccagtaccagtttttagaggatgctgttaaaaatcaaaggaa
ggttctttctgctctggtcaagcggcttggagataagcatacggctttacagaaatctactaaagatgttcgaacttcgattcgacaggtgagtgattta
cagaagagattgcaagtggatgtaaagatggcaatcttgcacataatgaaggaacttaataaacgtggaaaactactagtgaatgatatgcagaaagtaa
cagaaggtcatcaggataaactggaaaagcagcactgggctatgaacaaattgcaaaagcatcaagagcacatccttaggtttgccacgtgggcactgga
aagtgacaacaacatagccttattgctttcaaagaaactgatacattttcagttgcatcgtgctctaaaagttattgtagatccagttgagcctttaggt
gatttaaagttccagtgggactctgatacctggacaaagcatgcagagttttttggcaaaattgtatgcgacaaaacaggtgttcctcagtcatcttctg
gatcgttggcagcccagtctataaattctgcgtcatctcagatgatggatgactttgatgggtcttctgcag
3' EST: CX922569
[+]ttttttttttttttttgcacagacataactgattccacaaattcaaatgaaaccatttcccaacttctcgtagttacacagtgggattacaaataggcat
ttgaaatacagagcagtgcaaacagcaccccacccccaacgtgcccactaaataccattcagaaacatccatattcagtactggcatggaatcttaccct
gcttcatgccagatacgcgcccccccaccccacactcaactgcccactaaatagtgactatggcatcttacagcagcccatttggaatttgccagtccca
gaattcatcacttcacagtggaacaaggttcaattcatgcattttaaagaatgaacaagcttatgtaaaaagagggagggtatatttcccctataatatc
agtcacagaaaaaaagaaatgaaaaaaaaaaaaaaaaggggaccttctccaccaatttaaaaaatactcccacatcttatgcatttactaaatgtaatac
tgtatttgtgacaatgtttttaaaattacaaggagacttctctgttcaaactgatgaatgtccaaaaaggacccacaataaaagcaatatgatggcctta
ctgaaagatataaccactgtataaaccttgctgtaagagaggcttcataattcattagcagggatgcaccaaatccaggattcggttcggtattcagcct
ttttcggcagggttctcggctgaacccgaaaaactgggttcggtgcatccctattcattagtaggcttttgtggcaaaagatgcattacaacaagtaacc
aaaaaatgaaatggcatagtgaaat
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41414.jpg) |
Usage:
Suppliers: