XB-CLONE-3248345
Clone Name: IMAGE:7695023
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Gene:
atg2b
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC123022 [+]
AACATGGTGAATTTTGGCTGGAGTTTCATAGCGGCTCTTTGTTTAGTGTGGCAAAATATGAAGGCTTTGAAGACAAACATTATGTCTGTCTTCATTCAAG
CAGTTTGAGTCTCTACCATCAAGGTTTACTGGATGGAGGTATCCCTTCAGATGAGATCCGACTTCCTAGCATGACCCGTCCTCATTGGTTGGAGCCAACC
ATTTATTCATCTGAAGAAGATGGTATATATAGTCTCCCTGCTTCCAACGGAGTGGGTGTAGAGAGCCAAAGCATGTTGACGGTGGCAGTAAAAATTCAAT
CTGATAAGAGTGAAAGCAACACAAAGGAGTTCCTTGTTGCTATTGGACTGCGAGGAGCCACACTTCAGCACAGAGTTCTACCCTCTGAGGTCACCTGGCA
TGAGCAGGTATTACAATTTCTGAATATTCTGGATGAACCAGTTTTGGGTTATAACCCACCAGCTTCCATTACCACATTTCATGTTCACCTGTGGAGTTGT
GCTCTTGATCACAGGCCTCTCTACCTGCCTACGAGGGCCCTCTTGACTGTGGAAACCTTCAGCATTTCGTGTAGTGTTGCTTTGGATAAATCATCTTCAA
GCCTGCGAATCATCATGGAGGAGGCAGCGCTGCATTTGTCGGACAAATGTAATGCTGTAGCAGTCAATTTAAACAGGGATTATGTGCGTGTTCTAGACAT
GGGTCTTCTAGAACTAACTATCTTGTCAGTGAAGACTGGAGAAAAGTCTAAGCCACGGTTTGAGATGCACTGTTCTAGTGATGTTATACATATCCGGACA
TGTTCTGATTCCTGTGCTGCACTCATGAACCTCATCCAGTATCTTGCTAGCTATGGAGACCTCCACCCACCTTCCCGGACTGAAACCTCACCTGGTGCAC
ACAAGAAAAACAAAACGACCGATCCAGTAACTCAAGCAAATATACAAGGTCCAGTCCTGGCTGAGGCAGAGCAGCAGATACTGAGAGATTTGATGAGTGA
TGCCATGGAAGATACAGATCTTAACCATATTGTTTGCCCGTCAAAACCACATCTGAATGGAATTTCTGAAGGGAGATGTGAGACCCAGGAACCCCCATGC
TCAGATCTCTTTCTCTTCCCTGATGAAAGCGGCAATGTGTCTGAAGAATCTAGTCCCATGTTTCCTCCCACCACTCCCCATCTTCTTAGTGAAGCCATGT
TGGATTTTCCATCAGAAAGTGATGACTTCTGCATCCTGTATGCTCCAAAAAATTCTGTTTCGGAAAAGGATGAAGAACCATCTGTGAAGAAGCTTGTTGA
TGATAACATTTTAATAAAAGAAAATCACTTTAACCTGCCTCTGCAGAAAATGGACACAAGTAAAGCACCATTGCACTTTCCTGTGCCAGTTGTACGTTAT
GTGGTAAAGGAAGTGTCACTTGTTTGGCATCTTTATGGTGGCAAAGATTTTGGCAGTGCTTTACCACCACTATCACCTGCTAAACATTATATTCCTCATA
GCTCTCCATCAAATACCCCCACTAGGCGTGGGAACAGCTTAGTGCGTGGTGGACGAGGGAGAAATCAAGATCTTTTGATGGAAATACAATTTAGTAAGGT
TAAATTTCAGCATGAAGTATACCCTCAGGGAGCATCCACGGTAGAATCTCCATCGATGGAGCAACCAGTATCACGTCAGGTCTTTATTGTGCAGGATTTA
GAGATTAGGGATCGCCTGGCTTCATCTCAAATGAACAAATTCCTTCATTTATACAGCAGTAAAGAAATGCCAAGGAAAGCACATTCCAACATGTTAACTA
TCAAAGCCTTACATGTGCGTCCTGAGACTGGTCAGTCCCCACAGGAATGTTGCCTGAGAGTGACCTTAATGCCACTCCGACTTAATATTGACCAGGATGC
CTTGTTTTTCTTGAAGGATTTTTTTACAAATCTTTCTACAGAGGTTGAAATATTGGTCACGCCAGATCCAGAAGTAAAAAGATACCCAGCTTCTGATGTT
ACGTGCAGTTTGCCAAAGCACCTCTATTCTCAAGAGCGAAACCCTGTTATTTCATTTATTGGGCAAAGTGAGCCAGAGAAAAATGGCAGCATCAATTCTG
AACATATTGCACAAAGTGCCTCAGAAGAGGCATTCAGTGACCAGCCTGTCTTTTTCCGGGAATTTCGATTTACTTCTGAAGTTCCAATAAGGCTGGACTA
CCATGGAAAACATCTCTCAATGGACCAGGGAACACTAACTGGAATTCTTATTGGACTAGCTCAGTTGAACAATTCTGAACTCAAGCTGAAGAGGCTTTGC
TACCGACATGGTTTATTAGGTTTGGATAAACTCTTTTCCTATGCTGTTAATGAATGGCTTACAGATATTAAGAAAACCCAGCTTCCTGGAATATTGGGAG
GGGTTGGGCCAATGCATTCTCTAGTGCAGCTTGTTCAGGGACTGAAGGACTTGGTGTGGCTGCCTATTGAACAGTATAGGAAAGATGGTCGCATCGTCCG
AGGTTTCCAGAAAGGAGCTGCATCTTTTGGAACGTCTACTGCCATGGCAGCTCTGGAACTGACTAACCGAATAGTTCAAACTATTCAGGCTGCTGCAGAA
ACAGCGTATGATATGGTGTCCCCTGGACCAAGCCCTGTCGAGCTTAAGAGAGCAAAACGATTTTCCCATCATCGATTGGTGCATCAGCCTGTGGATCTAA
GGGAAGGGGTGGCTAAAGCATATACTGTTGTTAAAGAGGGCATTACAGATACGGCTCAGACCATCTATGAGACAGCAGCCCGTGAGCATGAAAACAGAGG
TGTGACAGGAGCTGTTGGAGGAGTTTTAAGACAGATTCCTCCTGCAGTTGTTAAACCTCTAATAGTTGCGACTGAAGCCACTTCAAATGTTTTGGGTGGT
ATGAGGAATCAAATCAGGCCTGATGTTCGAGAGGAAGAGTCTCAGAAATGGAGGCTGGAAGAGGGTTAGTTTCATGCTTTGTGGCTGTGCGTTTGCCTGA
CAAAGAAGTAATGGAAGACCCCCTCCCCCTCAAGGTTACAATTTGTGATGTTTCAGCAAATTTGGATCAGGAATCTGGTTACTTTTTTGATGACAAAAAG
GGAAAAAATAGACACTACATAGCTTTTGAAAATTATTATTTACACCGACATCTGTGACTTTGCCCCCCACTCATTCAGTTGTTCATCTGTATGCATGGTT
CTCTGAACAGGTTCTAAACTGGGGGTCATTTCAGCTGGATGGTTTCCATTCTTTTGGCATGTGTGCTATTTGCAACATACCCTCAGCAATTAATAGCTAG
TAATGAACCTTGCGTCCGAACAAACTTTCCAATCTGGCAGACGACAAAGTAATATAAGTGTGTTTGGATGTTTGCTTGATGGACTCCTTGGATTCTCCTC
TGCAATGCACTCTGGTTTAATGTTGTTTAATGAGTTGTTCTGTGTTGCTATTTGACTCTGGAAAGCCTGGATACAGCAGAAATATGTAAATTGTAGTCCT
TATTCTGTAAATTGTACTTGTTAATAGTGTGAATAAAGTTGACGTCCTGGTGACATTTGGTAAAAAAAAAAAAAAAA
5' EST: CX923297 [+]aacatggtgaattttggctggagtttcatagcggctctttgtttagtgtggcaaaatatgaaggctttgaagacaaacattatgtctgtcttcattcaag
cagtttgagtctctaccatcaaggtttactggatggaggtatcccttcagatgagatccgacttcctagcatgacccgtcctcattggttggagccaacc
atttattcatctgaagaagatggtatatatagtctccctgcttccaacggagtgggtgtagagagccaaagcatgttgacggtggcagtaaaaattcaat
ctgataagagtgaaagcaacacaaaggagttccttgttgctattggactgcgaggagccacacttcagcacagagttctaccctctgaggtcacctggca
tgagcaggtattacaatttctgaatattctggatgaaccagttttgggttataacccaccagcttccattaccacatttcatgttcacctgtggagttgt
gctcttgatcacaggcctctctacctgcctacgagggccctcttgactgtggaaaccttcagcatttcgtgtagtgttgctttggataaatcatcttcaa
gcctgcgaatcatcatggaggaggcagcgctgcatttgtcggacaaatgtaatgctgtagcagtcaatttaaacagggattatgtgcgtgttctagacat
gggtcttctagaactaactatcttgtcagtgaagactggagaaaagtctaagccacgggttgagatgcactgttctagtgatgttatacatatccggaca
tgttctgattcctgtgctgcactcatgaacctcatccagtatcttgctagctat
3' EST: CX923296 [+]ttttttttttttttttaccaaatgtcaccaggacgtcaactttattcacactattaacaagtacaatttacagaataaggactacaatttacatatttct
gctgtatccaggctttccagagtcaaatagcaacacagaacaactcattaaacaacattaaaccagagtgcattgcagaggagaatccaaggagtccatc
aagcaaacatccaaacacacttatattactttgtcgtctgccagattggaaagtttgttcggacgcaaggttcattactagctattaattgctgagggta
tgttgcaaatagcacacatgccaaaagaatggaaaccatccagctgaaatgacccccagtttagaacctgttcagagaaccatgcatacagatgaacaac
tgaatgagtggggggcaaagtcacagatgtcggtgtaaataataattttcaaaagctatgtagtgtctattttttccctttttgtcatcaaaaaagtaac
cagattcctgatccaaatttgctgaaacatcacaaattgtaaccttgagggggagggggtcttccattacttctttgtcaggcaaacgcacagccacaaa
gcatgaaactaaccctcttccagcctccatttctgagactcttcctctcgaacatcaggcctgatttgattcctcataccacccaaaacatttgaagtgg
cttcagtcgcaactattagaggtttaacaactgcaggaggaatctgtcttaaaactcctccaacagctcctgtcacacctctgttttcatgctcacgggc
tgctgtctcatagatggtctgagccgtatctgtaatgcccctctttacaac
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: