XB-CLONE-3248138
Clone Name: IMAGE:7695212
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Gene:
ptpn21
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154710 [+]
GATCTCGGGGCTGCTGGAGTGCTGCTTCCAATACTAAAGCTGGACGTGCAGCCTGGGTCAGTGGGGATCTGTGCAACCGCCAGCAGAACGGAGCTAGAAC
CTGCGGAGCGTTCCTAAGTGGGACTTTCTGTACTGCAATCCTGGATCTGTGTAACAGGATCTTGGATGTTCCCCACGTGCAGATACTTTTCTCTCTTTTT
AATGGCTGCTGGGTGACTTGGCTGACCTCGGAACCCTCCAGTTGATCCTGCTATTCAGGAAGATGCCTCTGCCTTTTGGGCTGAAGTTAAAGAGGACTCG
GAGATATACAGTATCTAGTAAGAGTTGCCTGGTGGCCCGGATCCAGCTGCTAAACAATGAGTTTGTGGAGTTCACGCTGTCAGTGGAGAGTTCTGGGCAG
GAGTGCTTAGAAGCTGTTGCACAGAGGCTGGAACTTAGAGAGGTAACTTACTTCAGTCTGTGGTATTACAACAAGCAAAACCAGCAGCGATGGGTGGATT
TAGAAAAACCACTGAAGAAACAGCTCGACAAGTATGCATTGGAACCCACTGTGTACTTTGGGGTGGTCTACTATGTGCCTTCTGTGTCTCAGCTGCAGCA
GGAGATCACAAGATACCAATATTACCTGCAGTTAAAAAAGGACATCTTGGAGGGGAAAATTCCTTGCACCTTGGAGCAGACTGTTCAGCTGGCTGGTCTG
GCTGTACAAGCGGACTTTGGAGACTATGACCAGTATGAATCTCAGGATTTCCTGCAGCGATATGCTTTGTTTCCTGTGGGCTGGATTCAGGATGAAAAAA
CGCTTGAGGAAGCAACAAAGAATGTGGCCTTGCTACACCAGAAATTGAGGGGCCTCTCTCCCCCGGATGCTGAATTATTGTATATGCAAGAGATTGAAAA
GTTGGAGAACTATGGAGATGAAAGTTACCCTGCCAAGGATAGCCAGGGTACAGACCTCTGCATTGGGGCTTGTCTCAGTGGCATATTTGTTAAACACAAG
AATGGCCGCCCACCTATTGTTTTCCGGTGGCTTGATATCGCAAATATGTCTCACAATAAGTCCTTTTTTACTCTTGAGCTGGCAAATAAAGAAGAAACCA
TTCAATTCCAGACGGAGGATATGGAAACTGCCAAATATGTGTGGCGGATGTGTGTTGCAAGGCACAAATTCTACCGGCTAAACGGCAATCTACAATCACA
GACTGTTAATCCTGTTCGTCGAAGATCTTCAACCAGAGTATCACTGCCAAAGCACCAGCCTTACATGATGCCTCCACACCAGATGCACTATAATGGACAT
TACGAGCCGTATACTTCTTCCCAAGACAATATCCCAATGTTTGTGAGTAGTCAGAATGGGTATTATTACCACTCCCAGACTAGCCTTGATCGAACACCAC
ATGATTACAATGGTAGAATTCGCAACGGGAGTGTCTATAGTGCACACAGCACCAACTCTCTGAACACCCAGCAGCACTACATGCAACCGTCACCTATGTC
ATCTAATCCTAGCATTACAGGAAGTGACATCATGAGACCAGAGTACATCCCATCGCACCGCCACAGTGCACTCATCCCACCCTCTTATAGGCCAACACCA
GATTATGAGACTGTAATGAGGCAACTGAGCAGGGGCATGGTGCATTCAGAGAGGCAAAGCCACTCAATGCGAAACCTGAACATTGGCAATTCCTATGCTT
ACAGCAGGCCTGATGCATTGGTTTACAGCCAACCTGAAATCCGGGAGCATGCTCAGTTCGCTCACCCCCAGTCCAATCACTATCCTTTTAACCTGAATTA
TAGTTTTCACAGCCAGTCCCCCTATCCATATCAAGCTGAGAGGCGTCCAGTTGTAGGAGCAGTAAGTGTACCTGAGCTAACCAATGTTCAACTTCAAGTA
CAGGATTACACAGCTTCAAACATCATGAAGACTCAGGTGTATCGTCCTCCTCCTCCATATCCTTATCCACGGCCAGCAAATAGCACCCCAGATCTTACTC
GACACCACATCAGCAGCAGCAATCCTGATCTCATCACAAGGCGAGTTCACCATTCCGTACAAATGTTTCAGGAAGACAGCCTACCAGTAGCTCATTCTCT
TCAAGAAGTAAGCGAGCCCTTGACAGCTGCCCGTCATGCTCGCCTTCAGAAAAGGAACAGCATTGAAATTGCAGGGTTAACTCATAGCTTTGATGGCCTG
AGGGTGAAAGAGAGGACCATATCCACATCTGCTGCCAGCGGGACACCACAGAGAATACTTTCAAGTGAATCTCAGACTGTCATCATGGAAAGAACGAAAG
AGGAGCAGGGTGAAGACTCTGAACGAGGAGGGTATGGTCACAAGAAATCACTTTCTGATGCCACCATGCTAATTCATAGCAGTGAGGATGATGATGAAGA
GACCGAAGAAGATAACCGAACCAGCATTACTCAAGCGCCCTTGTTCTATGATAGGGCACAGCTTCGTACTGTAATGGAAACATACTCTGATGAGCTGGAA
CCAAATTACACCTACAGCTCTGCTTCTGCCAATCATCTGCACATATATGAGCACAAAAAACAAGAGGAGGTGGAGAAGGAGATGAGGCCTAAAGTTGTAA
GCCCAGTGCAATTTTCTGGTGATTTTCAACCTAATAGGAGGGGAGAGGGCAACATACCGCCTTCAGCATCTGAGTCAGACCTCACTGCAGTCAGCATAAG
GTATAGACCTAAGAAGGATCCAGTAAAAAAGAGGCCAGTGTCTGAAGTCCTCTCAGGAAAGAAGGATATTGTGGAAGGACTGCCACCTTCTGGAGGAATT
AAAAAGGGCACCCGAATGGAAGCCAAAAAAATTGGTCCTCTGAAAATGGCTGCATGGAATGGTTTATCTTTGTCCCGTATGCCTTTACCAGATGAAGAAA
AAGAGGAACCCTCCAGGGCTTCTAATGATGACAGGTGTAAACTGCTGGAACAGAGACTGGAGCAGGGCATGGTGTTCACAGAATATGAACAGATCAGCAA
GAAGAGACTGCCAGACAGTGAGTGCTCCATTGCACGTCTTCCTGAGAACTCGGAACGAAATCGATATCAGGATGTGCTGCCCTATGATGATACTAGAGTA
GAACTGGTTCCAACCAAAGAAAACAACACTGGTTATATCAATGCCTCCCACATTAAGGTGACAGTTGGTGGAGTTGAGTGGGATTACATTGCCACACAAG
GCCCAATTCAGAGCACATGCCAGGATTTTTGGCAGATGGTCTGGGAACAGGGAATTGCCATAATCGCCATGGTAACGCTGGAAGAAGAAGGTGGTCGTGA
AAAAAGTTATCGCTATTGGCCTAGACTTGGCTCAAGGCACAACACTGTCAATTATGGGAGGTTTAAGATCACCACCCGGTTCCGAACTGACTCTGGTTGT
TATGCCACCACGGGCTTAAAGATTAAACACCTTCTGTCTGGCCAGGAGAGGACAGTGTGGCACCTTCAGTATACAGATTGGCCAGAACATGGACATCCAG
AAGATGTGAAAGGATTCCTCTCCTATTTGGAAGAGATCCAGTCTGTTCGCCGTCACACAAACAGCACAATCGATCCCAATAGCCCAAACCCCCCAGTGCT
TGTGCATTGCAGTGCCGGAGTTGGTCGGACAGGGGTGATCGTTTTGTCTGAAGTTATGATTGCATGTTTGGAACATAATGAGATGCTGGACATCCCCCTA
GTCCTGGACATGCTGCGGCACCAGAGGATGCTAATGGTGCAGACTCTTTCTCAGTACACTTTTGTATATAAAGTCCTGATTCAGTTTCTGAAGAGTTCCC
GGCTGATCTGACCTCAACTGGAGTCTTGCCACTGATGAAAGCAGCAAGAAGAGCTCATGAAAGCTCATTTCTTTGTAAAATGGGATGCCCATCCAAGTGG
GCAAAAGCCACATACAGTGCCATCCATATGCAAAATAAGCCATGTATTACTGTGGCCAAGACCAAGTATATGGTGCTCAACCTGCATTCTTTGGAAGGCT
TGGATGTCAAACAAATCCACATATTTGAGATTGAACTCTGAAATCAGCAGTTCAACACTGGGTTCTGGCAGTTTGGTATATCTAAGTAGTGGTGACCCAG
TGTTTTTCCAATTTGAGTCCTCTAATGAGATTCTGAAATGTAAAAAGGGAAGATCGAAATATTCTCCTGGCGTTTGTTTGGAGTCAGACTGACCACTGGT
ATTGTCTCGAGATCTATCAGCTTGGATACTTCAGTTTAACCCCATTGCTGAAACTACTGGGTTAGCAGGACAAAAGCTCATTTTATCAGTGTTATTATGC
AAAGCTCTGATTTTACTGGTTGAAATCATCTATGATGCTAATGAAGTTTTGAAGCACTAAAAAAAAAAAAAAAA
5' EST: CX923636 [+]ggggctgctggagtgctgcttccaatactaaagctgggcgtgcagcctgggtcagtggggatctgtgcaaccgccagcagaacggagctagaacctgcgg
agcgttcctaagtgggactttctgtactgcaatcctggatctgtgtaacaggatcttggatgttccccacgtgcagatacttttctctctttttaatggc
tgctgggtgacttggctgacctcggaaccctccagttgatcctgctattcaggaagatgcctctgccttttgggctgaagttaaagaggactcggagata
tacagtatctagtaagagttgcctggtggcccggatccagctgctaaacaatgagtttgtggagttcacgctgtcagtggagagttctgggcaggagtgc
ttagaagctgttgcacagaggctggaacttagagaggtaacttacttcagtctgtggtattacaacaagcaaaaccagcagcgatgggtggatttagaaa
aaccactgaagaaacagctcgacaagtatgcattggaacccactgtgtactttggggtggtctactatgtgccttctgtgtctcagctgcagcaggagat
cacaagataccaatattacctgcagttaaaaaaggacatcttggaggggaaaattccttgcaccttggagcagactgttcagctggctggtctggctgta
caagcggactttggagactatgaccagtatgaatctcagggattcctgcagcgatatgctttgtt
3' EST: CX923635 [+]gcttcaaaacttcattagcatcatagatgatttcaaccagtaaaatcagagctttgcataataacactgataaaatgagcttttgtcctgctaacccagt
agtttcagcaatggggttaaactgaagtatccaagctgatagatctcgagacaataccagtggtcagtctgactccaaacaaacgccaggagaatatttc
gatcttccctttttacatttcagaatctcattagaggactcaaattggaaaaacactgggtcaccactacttagatataccaaactgccagaacccagtg
ttgaactgctgatttcagagttcaatctcaaatatgtggatttgtttgacatccaagccttccaaagaatgcaggttgagcaccatatacttggtcttgg
ccacagtaatacatggcttattttgcatatggatggcactgtatgtggcttttgcccacttggatgggcatcccattttacaaagaaatgagctttcatg
agctcttcttgctgctttcatcagtggcaagactccagttgaggtcagatcagccgggaactcttcagaaactgaatcaggactttatatacaaaagtgt
actgagaaagagtctgcaccattagcatcctctggtgccgcagcatgtccaggactagggggatgtccagcatctcattatgttccaaacatgcaatcat
aacttcagacaaaacgatcacccctgtccgaccaactccggcactgcaatgcacaagcactggggggtttgggc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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