XB-CLONE-3247818
Clone Name: IMAGE:7695617
|
Gene:
phrf1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX924163 [+]
cagaatttcccaggataccaaagatcaaaaaggaaacggacagtagccccagtaatttgagaggaaagccttcagactctgcgaatagcccaagtacaaa
taccaaccatcccagcatctgcataaatcaactaacaggtcggggagagagtaacacattgggaaagtttaacaccacagaaaacagaataaaaaacact
cgacaagagtcccaggaccagtcaaggtatagttctggcaattacgctggtcattcaggtacaagcagtagcactgcaccaagaagtgtggggtcattag
atgtaggcggtggaggtcttcgcataacaatcagtggaaattctggcagttcttgtaggcaatttagtccgagttcaaaggatcctttccgttcttctga
tggtaaaacacaaccaaaaggagctcttcctgttccctttccttccaccaagagggaaaaacctgttaaaaatgaaatatatgacccgtttgaaccaact
ggttcagattcaggctctcgtgatagtagcccagaaagacctgggccgtcatctcagccttcagcagcagcttctgcaacaataaaaacagaaactgcta
ttgcagccacatcaagtggagccaaagtgggaacatttcgaagttttaggctgctcccttcacactccactaaggtgggagtgtcaaaattgggaccaga
tttctcggatctctctcccgctagcaaggatcaaca
3' EST: CX924162 [+]tttttttttttttttctggcaattgttgtcctttatacatattgtagggtcaaaggacaaaatctttatttcagaatgtattctttcatcgccctgcatt
tgttgcgccatgtccccctcttacttcctttgcttgccatgcgaaaaaaaaaaaatctgatgcacggcttaacaaaaattcctttctcttacaaatgagt
ttacttggtcctccagggagacgttctcaacacagggaccgccaagatggcagcagggtttccgaacaagtcgttgattgatgtaaaaaatagggtgcaa
tcttgatttcttctttttccccctctatgtttcacaccttgaaacttctgcataggcaggaaaataacctctttttgctggcagagggccctgccatctg
tacactgaagtccaaagaaaccaaactgggactggtgaaaacaaaggattgggtgtcttgaaatgagcagaacatcaccttcatgtacgaaactaatgat
caacttcaactgcacaggcggagccccatgcagcccaactctgctgcaccgtcgctgaggctgagcacttttacatctgttttcatacgacaaagacttt
tcttctcatagtggagaatcacgcatagtcccatggtcctcaaacgactccgacttcttgtgttttcgtgcgtgtttgtacttatccacgtaggctttca
ccaggttaactaccttaactggatttatttctccactcttgctgtggcagatcttctgtacagctttgc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: