XB-CLONE-3247772
Clone Name: IMAGE:7695292
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Gene:
cdkn2d
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121960 [+]
AAACCAATGGGCACAAAGAGACGACGGCACAACAGTTGCCTTTTTTAAAAAAAATAACACGCGTACCACGTGGGGCGGTGACGTCAGAGGCCGAAAACAA
GCGACAGGCTGCGATCCGCCGCGACTCGGACTGCGCCCGTTGAAGGTTCTGACTGCGCGTCTCTCACCTGCCGGGCAGCGGGTCACGTGTTCAGCACCTG
TGTCAGCGCGGGAAGGCGGGTATATAAGCTCTCCCTCATGTTGCTACAAGAGACCAGCGCCGGAGACCGCCTGACACGAGCGGCTGCCAGGGGAGACCTA
TTAGAAGTGAAGAGGCTTCTCCATGAAGAGAGGATCCACCCTGACTGCCTAAACCGTTTTGGCAAAACTGCTCTGCAGGTCATGATGTTTGGCAGCACCC
CTGTTGCATCAGAGCTTTTGAAACAAGGTGCCTCTCCTAATATTCAAGATGCATACGGCACCACCCCTGCTCATGATGCAGCCCGTTGCGGATTCCTGGA
CACCTTACAAGTTTTGGTCCAGCATGGAGCGGAGATCAACACACCTGATGCCTCTGGCTCCCTACCTATACACCTAGCCCTTAAAGCAGGTCACGTCCCT
GTTATTACCTACCTTGCTCTTATCTCCGACCTTCAGCATCGGGATCGGGAAGGGCACACGCCATCACAGTTGGCCGCCATCTTGGATCCTCGCCTGGCTT
CCATATTTGAGCTCAGCACATAGAGGAAATACCCAAAGTTGTCCAAACATGCCATGGGAACAATTTGATATGGTGCTCAGTTTCTTTTCCTAGTGCAATG
CATAGTAGTGGGTAGCTACTCAGTATTATTGTATAGTTTAGCATGTTTATGACTGACTGCGGAGAGCTGCAATAGTACCAATGCCCTATATGTTGTCAGG
AGTTTATAAATGCTGGGAACAACTGCAGCACCCTTCAGGGAAGGGGAACATTTACTAGTGGTTAAATGCTGTTGGTTTTAGGGTAATAACAATGCATTAA
TGAGCACTTCTTTGTGTACATATTTACATACATGTGGCCGGTTACGTGCAGTTTTTTTATTTTAATACTCAGGTCATGTTTAATTATTATTATTATTTTT
AATAATTCTTTTCTCTCTTTGAAAGTCCCTGTCTTTCTGCAATTACTTTATACTTTTATATGTTTATGTTTAGTGAAAGTTTCTAAATTACAAATGCATT
TATTTTAGATTTCAGTACAGTCCAAACCCCCCCAAAATATGATTCACAAATCAAAACCAAAATGGTAACCAATTACTCCTTATTGCTCCATATTTTCCAA
TACAAAAACAATTGGGCCCATTTTCAGTGGTACAGGCATTACTGTACTGTGCGGCATTACAGGGGCACCCAAGTGCTGCTCCATGCTCTTTCAGTATACA
GCGCAGCCGCTCAGGTGCAGCTCTTTGTGTTCTATTCTAGGAGTACAAAGAGGTGTGCCTCGGTGTAACCAGGAAATTTCAGTGGAACAAGATGCAAAAT
AAAATAGTGCTTTGTCCCTGTTTTTGTACTTTGTAATGTACCTTGCCCTTAATGGCAGAAGTGCAAAAAAATGCCCCTTTGTTTAAGCACGGAGGTCCAG
TGTCTTTATAACCACCTCCTTAATGTCTCTGCTTCACATAAGCCCTACATGTAACTCAACCTTTCCCAAATAAGATTTTCCTATGACTCTCCTTAAACCA
CTCCTATTGCACAATATAACCCCTCCATATAACTCCTCCTATTGCTCCATATAACCCCTCCCTATAACTCCTCCTATTGCTCCATATAACCCCTCCCTAT
AACTCCTCCTATTGCTCCATATAACCCCTCCCTATAGCTCCTCCTATTGCTCCATATAATCCTCCCTATAACTCCTCCTATTGGTCCATATAACCCCTCC
CTATAAGTCCCCCTATTGCTCCATATAACCCCTCCCTATAACTCCTCCTATTGCTCCATATAACTCCTCCTATTGCTCCATATAACCCCTCCCTATAACT
CCTCCTATTGCTCCATATAACCCCTCCCTATAACTCCCCCTATTGCTCCATATAATCTCTCACTATAACTCCTCCTATTGCTTCATATAACCCCTCCCTA
TAACTCCTCCTATTGCTCCATATAACCCCTCCCTATAACTCCTCCTATTGCTCCATATAACTCCTCCTATTGCTCCATATAACCCTCCCTATAACTCCTC
CTATTGCTCCATATAACCCTCCCTATAACTCCTCCTATTGCTCCATATAACTCCTCCTATTGCTCCATATAACCCTCCCTATAACTCCTCCTATTGCTCC
ATATAACCCCTCCCCATAACTCCTCATTTTCCCATATTCCCTCTTTGTATAATGCCCTTCTCCTGCTCCAGTTGACTCCTCACTTGTGGATGATATAGCC
CCATAATAACACCGCCTGTAATTCTTACTTGTACCCACCTTTTCCCTATACGCGGCTCGTTTCTGCGCCTAGCCTTGTATAACCTTGCCGTACAATTAAG
TTAATTGCATTATATAAAAACTCAGTACAATTCCTCTGTTGCTGTATCAGCAAAAGTAAAATGCCATTCCGGCACAGAGGAATGTAGTTGTGGGTAGGCG
GCCCTGCAGAGAAAGAATTTCTGTGTTCCTTCCCACATAGAGCCGTTCTGCAATGTGCTGATTCCGCCATAGTCTGCTCATTAGAGATTCCTACAGGCCG
TGTGGGAGGCAAATGTCAGCCCCTCTGTCACTGCTTTAGGGACAACAGAACCTAGACAGGGATCAGGTGACTGCCCAAAATATTACCCAGACACCCCAGG
GCCACAGCTTGGGCAGCACTTATATAAAGGGCCCTGCTTCTCAAGTGTAAGGAGTCACATTTATTAGTGCTTTGATTTCCGTTGGAGTTGCCTATTTATG
GAGGAGGTAATGGGATTCTGCCATCTCTGAACTATACAAAATAGTAAAATTCTGCATCTATTCCATGTTTCTGCTATAGTGATGCTTTATACTGGCGCCA
TCTTCCCTTCCAGTCCCAATCTGTCATTATTATTATTATGCCACTGTGGCACCCCAGTTGTGCCCTTTGGCATCCCCACAGCTTTGCCTTTAGAGACATT
TACTAAAACACACTCCCTGATGTCTCTTCCTAATATACTGTAGTAACGCGCGGGAATATTACTTGTGCTTTGGACATATTGCATTTAATTGAAGTGTATC
ATAGAGCTTTCTGTGCTGATTAAAGGGAAACTATACCCCCAGAATGATCACTTATCCAACAGATAGTTTATATCATTGAATCTGGCCAAACTGGAATATA
TATCCGTAAATATTGGCCTTTTACATCTTTTCCCTTGAGCCGCCATTTAGTGATGGGCTGTGTGCTCCCTCAGAGATCAGCTGACAGGAAGTGATGCAGC
TGTAACTGTAACAGGAAGTAGTGTGGGAACAAAAGGCAGAACTCTGTCCATTCATTGGCTGATGGGGCCTAGCATGTATGTGTGCCTTGGCTTGTTTGTG
TGCACTGTGAATCCTATGATCCCAGGGGGCAGCCCTTAATTATTAAAATGGCAGTTTTCTATTTAGGATTACCCAATGGCACATACTGCTAATAAAGTAT
ATTTATATGAAAATGGTTTATTCAGATGAAGCAGGTTTTACATTTGGGCTGTTTATGCAATATATTTATAGAGACCTACATTGTTTGGGGTATAGTTTCC
CTTTGGGCAGTCACACACAGGGCTATTTCAGGTGCTTTAAAGGGGATCCATAGTGATTTATTGATGTGAACTTAGAGTTAAATGAATTGTCTGGAAATTA
TCAGGCTTTTGGCTGAACAGCTTCTCTGAAAGTCATGGTAACTGGCTGTACTGACCCTTTGCAATTGTTTTTTATTTTTTATGTTTTTTTTAGTTATTTA
GCTTTATATTCGGCAGCTCTCTATTTGGTATTTGAGCAGCTAGCTGGTTGCTAGGGCCTTATTTACCCTAGCAACCAGGCAGTGGTTTAAAAGAGAGATG
GGACTATGAATAGTAGAGAGCCTGTACGGAAAGATAAGTAATAGAAACTAACTATAATAATGAAACTGTAGCCTCACGGAGCAATAGCTTTTGGCTGCCG
GGGTCAGTGACCCCCATATGAAAGCTGGAAAGAGGCAGAAGAGAAAGGCAAATATTTCCAAAACTACTTAAAAAAAAAAAAAAA
5' EST: CX924228 [+]aaaccaatgggcacaaagagacgacggcacaacangttggccttttttaaaaaaaataacacgcgtaccacgtggggcggtgacgtcagaggccgaaaac
aagcgacaggctgcgatccgccgcgactcggactgcgcccgttgaaggttctgactgcgcgtctctcacctgccgggcagcgggtcacgtgttcagcacc
tgtgtcagcgcgggaaggcgggtatataagctctccctcatgttgctacaagagaccagcgccggagaccgcctgacacgagcggctgccaggggagacc
tattagaagtgaagaggcttctccatgaagagaggatccaccctgactgcctaaaccgttttggcaaaactgctctgcaggtcatgatgtttggcagcac
ccctgttgcatcagagcttttgaaacaaggtgcctctcctaatattcaagatgcatacggcaccacccctgctcatgatgcagcccgttgcggattcctg
gacaccttacaagttttggtccagcatggagcggagatcaacacacctgatgcctctggctccctacctatacacctagcccttaaagcaggtcacgtcc
ctgttattacctaccttgctcttatctccgaccttcagcatcgggatcgggaagggcacacgccatcacagttggccgccatcttggatcctcgcctggc
ttccatatttgagctcagcacatagaggaaatacccaaagttgtccaaacatgccatgggaacaatttgatatggtgctcagtttcttttcctagtgnca
tgcatagtagtgggtagctactcagtattattgtatagtttagcatgtttatgactgactg
3' EST: CX924227 [+]tttttttttttttttagtagttttggaaatatttgcctttctcttctgcctctttccagctttcatatgggggtcactgaccccggcagccaaaagctat
tgctccgtgaggctacagtttcattattatagttagtttctattacttatctttccgtacaggctctctactattcatagtcccatctctcttttaaacc
actgcctggttgctagggtaaataaggccctagcaaccagctagctgctcaaataccaaatagagagctgccgaatataaagctaaataactaaaaaaaa
cataaaaaataaaaaacaattgcaaagggtcagtacagccagttaccatgactttcagagaagctgttcagccaaaagcctgataatttccagacaattc
atttaactctaagttcacatcaataaatcactatggatcccctttaaagcacctgaaatagccctgtgtgtgactgcccaaagggaaactataccccaaa
caatgtaggtctctataaatatattgcataaacagcccaaatgtaaaacctgcttcatctgaataaaccattttcatataaatatactttattagcagta
tgtgccattgggtaatcctaaatagaaaactgccattttaataattaagggctgccccctgggatcataggattcacagtgcacacaaacaagccaaggc
acacatacatgctaggccccatcagccaatgaatggacagagttctgccttttgttcccacactacttcctgttacagttacagctgcatcacttcctgt
cagctgatctctgagggagcacacagcccatcactaaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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