XB-CLONE-3247609
Clone Name: IMAGE:7695276
|
Gene:
kremen1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC161114 [+]
tctgatttcagatttctcaaccagtaacatgattaggaaagaaaacatgagggacaaacgcaacatgtcaaaacaatgtacatgcacaataaaaagctgt
aatagccaaatgaaccatgaggcacaaacagtgaatcagacctttaataacccggaggaaagtttatcctggatgcactgagcttccaagaaacagatgg
aaacgattcatggcgacaggtttaaaacaaacactgcagtttgctgtgatagtttgttggagcagactgtagagagtggtcttcaaaagagtgctacact
gtaaacggggctgattacagagggacacagaaccagacatccctagatggtgggaagccttgcctcttctggaacgagaccttccagcatccatacaaca
ccttgaaataccctaatggtgagggaggcctgggagaacataactactgcagaaacccagatggtgacgtgagcccctggtgttacattccagagcaaga
agacggcgtgtactggaagtactgtgacattccaacctgcaaaatgcctggcaacctgggctgcttcagagatcatgggaacccccctcctttgactggc
atcagcgagacatctaataaacagaccatacaaacctgcattactatgtgccggaggcagcgctacaagctagctgggctggaggcaggatttgcctgtt
tttgtggtaacaacgccgactacaggaagcatggagagatgcccagcacagactgcaacagtgtatgttttggggatcacacacagccatgcggagggga
cgggcgcatcattctctttgactccctgattggagcctgtggtgggaattataccatgaattctgcagttatttactcaccggatttccccgacagttat
gggactggaaaagcctgctactggacaattcaggttacgggcgcctcgcttatccgctttaacttcacttttttcgacatcaaagactccagagatatgg
tggagctattagatgggtacaccaagcaagttctgatacggtttgatggcaggaaccacccaacacactcgttcaacatctcccttgattttgtcatttt
atatttcttctctgatcgcattaaccaggcacaaggattttctgttgtatatgaagctttcaaggaggaaagcctagaaaaacccaactcctccagcaac
cagacgcagacagaactgatcactcagacaaccaacctcagtattaacgcagctcgctcttcaaagatcctttatgtcataacaaccagtcctagccgac
caagtgggcacgtgccgggctggacagtctatgcacttacaggtctcctcattttgaccatcattgcagttttggctaaagcacttttacatatatcaat
gaagtctgccaggttggcatcgccatctagcctggacagctgccacagaggatccgcaggagaaatttggagcatattctacaagccatccacatctatt
tctatttttccaaagaaattaaagggtcagcatgatgaccgcaaccctttggtaggagaataagttctgctggaggattttcagtattgctgggcttccc
gggggaaactcctgccacatctgtatttggacatctttcacaacagaccatggacagcagatgtcagctgcacggtatcttcctggcattgtgactcaag
ttgagagagacgtacctactgtccaagatgtgctgtggcaccttcatacaagatccacaaagagtgagatcggataagcttgctggtgttgctgaattag
gaaaccccctatctagcctctaattcagcacatggacaacatgtggtttgtgttgggatggaaccacagcttaatgctgcaagcgttacatgcaatcatc
cctgctctgtgcagcggggtagcccatatttgacatgacgttggacttgccttcagctcctactttttatattgtatttatgaacttacagctcttaaag
gagcaacggaggttgtctggttttctcatgatctacttcatcaagagctactgtgatggagttttgtactgtgcaaatgtacatattgttacactgagca
aacaaaaaccaccagaaatacacacaaatcaaagcttcagccatctttctatgagccaaactagaacaggtttcccatcattcccagagtttacactgtc
tgatacattgaatgaatgaattctgtacgagggactgaaggttcaggaggacctgcatgaaatgggtctcggtgtttatggggccagaacattcagtaga
tccctgtatgcggtatatttgagaattgcatatctgtatccatttaagatgattgtgcacattatcaggactcagaatccattctcatacacaagccttt
gtctttttaactgaatatgtggaagcagattttcagcttgttccataaaatacgcttcgataataaaaaaaaaaaaaaa
5' EST: CX924470 [+]tctgatttcagatttctcaaccagtaacatgattaggaaagaaaacatgagggacaaacgcaacatgtcaaaacaatgtacatgcacaataaaaagctgt
aatagccaaatgaaccatgaggcacaaacagtgaatcagacctttaataacccggaggaaagtttatcctggatgcactgagcttccaagaaacagatgg
aaacgattcatggcgacaggtttaaaacaaacactgcagtttgctgtgatagtttgttggagcagactgtagagagtggtcttcaaaagagtgctacact
gtaaacggggctgattacagagggacacagaaccagacatccctagatggtgggaagccttgcctcttctggaacgagaccttccagcatccatacaaca
ccttgaaataccctaatggtgagggaggcctgggagaacataactactgcagaaacccagatggtgacgtgagcccctggtgttacattccagagcaaga
agacggcgtgtactggaagtactgtgacattccaacctgcaaaatgcctggcaacctgggctgcttcagagatcatgggaacccccctcctttgactggc
atcagcgagacatctaataaacagaccatacaaacctgcattactatgtgccggaggcagcgctacaagctagctgggctggaggcaggatttgcctgtt
tttgtggtaacaacgccgactacaggaagcatggagagatgcccagcacagactgcaacagtgtatgttttggggatcacacacagccatgcggagggga
cgggcgcatcattctctttgactcccctgatggagcct
3' EST: CX924469 [+]atcgaagcgtattttatggaacaagctgaaaatctgcttccacatattcagttaaaaagacaaaggcttgtgtatgagaatggattctgagtcctgataa
tgtgcacaatcatcttaaatggatacagatatgcaattctcaaatataccgcatacagggatctactgaatgttctggccccataaacaccgagacccat
ttcatgcaggtcctcctgaaccttcagtccctcgtacagaattcattcattcaatgtatcagacagtgtaaactctgggaatgatgggaaacctgttcta
gtttggctcatagaaagatggctgaagctttgatttgtgtgtatttctggtggtttttgtttgctcagtgtaacaatatgtacatttgcacagtacaaaa
ctccatcacagtagctcttgatgaagtagatcatgagaaaaccagacaacctccgttgctcctttaagagctgtaagttcataaatacaatataaaaagt
aggagctgaaggcaagtccaacgtcatgtcaaatatgggctaccccgctgcacagagcagggatgattgcatgtaacgcttgcagcattaagctgtggtt
ccatcccaacacaaaccacatgttgtccatgtgctgaattagaggctagatagggggtttcctaattcagcaacaccagcaagcttatccgatctcactc
tttgtggatcttgtatgaaggtgccacagcacatcttggacagtaggtacgtctctctcaacttgagtcacaatg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: