XB-CLONE-3247603
Clone Name: IMAGE:7695612
|
Gene:
lonrf2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX924483 [+]
tagttcagcaacaactgaataaagtttggttgagcagtgcagggcagcaaggtttacaacccctttaaagacagggacattcagggtaacttttaacaga
aatccatctgattttattcaacaaggttgtaaagagaaatacattgtaacagtgcataacaaaagtaacaactcacagtctctgttcattaaaggggaac
tccacccaaacacaaattaagctttttgaaagcataatttcaagcaactttgcaatatatatctatgcagccttttcgtgatttttaatgtaataatatt
gtttggaacagttacctaagcctaaggctgactgctttaaggctaaaaataaaatataactgtagttgcctggcctcgacctgcctttagcctgtagcct
ccaaatccctgcacatgtgatatcaataaggaaaggaatatcgcagtacaatgcactgtgggttatatagttcctgcatgctgtctgtaagctgtggaga
taatgttacaatttgttttagttccacctcccctgccaggattaaaaataatgcagaaatacaactggtttgcagctggatttcatacacatagcctttc
ctcatatgctgagtctcagagagcattctgggagatatgtacttccctccagagttttcccagtcctgtgccacaagttacttgccattaacagtaatgc
tgtatagtttgtggccagcgatttttttacctgaaaacttctgtttttatgactcttgcaggcagagggatgtagaaagtagaatgtagatgtagaaagt
cactctaggcagtcgctg
3' EST: CX924482 [+]tttttttttttttttagaaaaattgcttatttagatgaagcagtgttttatttgagctgtttcaagcaatatattttatacagacctacattgttcaggg
gtacacatttcctttagagctaaagtgtatgggacactttgtcaggaacagcgactgcctagagtgactttctacatctacattctactttctacatccc
tctgcctgcaagagtcataaaaacagaagttttcaggtaaaaaaatcgctggccacaaactatacagcattactgttaatggcaagtaacttgtggcaca
ggactgggaaaactctggagggaagtacatatctcccagaatgctctctgagactcagcatatgaggaaaggctatgtgtatgaaatccagctgcaaacc
agttgtatttctgcattatttttaatcctggcaggggaggtggaactaaaacaaattgtaacattatctccacagcttacagacagcatgcaggaactat
ataacccacagtgcattgtactgcgatattcctttccttattgatatcacatgtgcagggatttggaggctacaggctaaaggcaggtcgaggccaggca
actacagttatattttatttttagccttaaagcagtcagccttaggcttaggtaactgttccaaacaatattattacattaaaaatcacgaaaaggctgc
atagatatatattgcaaagttgcttgaaattatgctttcaaaaagcttaatttg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: