XB-CLONE-3247505
Clone Name: IMAGE:7695925
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Gene:
ddx6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122944 [+]
GGAGGAAGGAAGCCATTTTGGAGCTTCGAGTAAGAGAAACACACGGAGAGCAAACAAAAACATAATAGTTATACTTATAGTGAGTAATAAACAAAGAGAA
ACGACGGAAACAAACGGACAACACGACGACGAGGAGTCCGACGTGTCCGAGCCCGGGATTGACTTGAAGGAACGGACTTTATTCTTAGTTTTGGGCTTTT
TTTTTAATAAAGGAAATTGATTTTGTGCACCAGAGCAAGTGATTTCACTGTTGACAGACTTTAAGGACAAGCACTTGCTTGTGTTGCAATAACAAAGACT
CATATATTGAGCAAGACTTTATCTCTTCATTTCGAAGAGTCAAATAAACTTATTACAGTCTCTGTACTGACTTTAAACCTTGTGAAGCTGAAAAGACACA
CCTTCAAAGGGACAATTTAATTAAATACAAAACAAAATGAGCACCGCCAGAACCGAGAACCCTGTTTTAATGGGTATGTCCAGCCAGAATGGGCAGCTAA
GAGGCCCATTGAAACCCAGTGCAGGGCCTGGAGGTGGGGGGACACAGACACAACAAATAAACCAGCTGAAAAATGCCAGCACAATCAACAGTGGCAGTCA
GCAGCAAGCACAGAGCATGAGCAGCGTTATAAAACCTGGTGATGACTGGAAGAAGACATTAAAACTCCCTCCAAAAGACCTTAGAATCAAGACTTCGGAT
GTCACCTCCACAAAAGGAAATGAATTTGAAGATTACTGTTTAAAAAGAGAGCTGCTCATGGGGATATTTGAAATGGGATGGGAGAAACCATCTCCAATAC
AGGAGGAGAGCATTCCCATTGCATTGTCCGGTAGGGATATCTTGGCTAGAGCAAAAAATGGAACTGGCAAGAGTGGAGCCTACCTCATTCCCTTACTTGA
ACGGCTAGACCTAAAGAAGGACTGTATACAAGCAATGGTGATTGTTCCTACTCGAGAACTGGCACTACAGGTCAGTCAGATTTGCATCCAGGTCAGCAAG
CACATGGGCGGGGTCAAAGTCATGGCGACAACAGGGGGCACAAATCTGAGAGATGATATAATGAGGCTTGATGATACAGTACATGTAGTAATAGCAACTC
CTGGCAGGATTCTGGATCTCATTAAAAAAGGTGTGGCTAAAGTAGACCATATCCAGATGATCGTGTTGGATGAGGCAGACAAACTACTGTCTCAGGATTT
TGTGCAAATAATGGAGGATATAATTATCACGCTACCTAAAAACAGACAGATTCTACTTTACTCAGCCACATTTCCCCTCAGTGTTCAGAAGTTTATGACT
TCCCATTTGCAAAAACCCTATGAGATTAACCTGATGGAGGAATTAACGCTGAAGGGAGTAACGCAATATTATGCCTATGTGACTGAACGGCAGAAAGTTC
ACTGCCTCAACACACTGTTCTCTAGGCTCCAAATCAACCAGTCAATAATTTTCTGCAACTCCTCCCAACGGGTTGAACTCCTGGCTAAGAAAATATCTCA
GTTGGGCTATTCGTGTTTCTATATCCATGCAAAGATGAGACAAGAGCACAGGAATCGTGTTTTCCATGATTTCAGAAATGGTTTGTGCCGAAATCTTGTT
TGCACTGATCTGTTTACCCGTGGTATTGATATCCAAGCTGTGAATGTGGTGATAAACTTTGACTTTCCAAAGCTGGCGGAGACATACTTGCATCGCATAG
GAAGATCAGGTCGCTTTGGCCACCTTGGGCTTGCCATAAACTTGATCACTTATGATGACCGATTCAACCTTAAAAGTATTGAAGAACAGCTGGGAACTGA
AATCAAACCCATACCCAGCAGCATAGACAAGAGCTTATATGTGGCAGAATATCACAGTGAATCTGGAGAAGACAAACCTTAACCTGAACGCTTTGTCACT
GAGACCCGTTGAAGACAAGAAGATCTTGTCCTTACATGCATCACTTAAGAGGGTTTCATCCCCACATGCCTTTTACTTTATATTTAGAGCTTCCCACCCC
TTCTGGAACACTGACCAGCATATATCAAACCTGCATTGTCATCATCTTCTCCCTCCCCCTTGTCTCAGGACAGTACCCTACAAACCCCAAATGTTTGCAC
TGCGTTTTCACCTTTTAGTTGCACTTAATGCCCCCCCCTTTTTTTTTTTTTGACATGTTTCTCATGGGGATGGGAAGCTAACCCTGTTGTCCATTTTGTT
TGGAGGGGCCTAAAAATTAGGTACAGCCTTCTGCTTCTAAACATTTTGCTTCCAGATGACCCAGTATTACTGCTTAAGACATTTCAGGATCCTCTGGCAG
CAACAGTTCTAATACATACATATATATATAATTTTTTTTTTTATCCCCCCTGTCTGCATCCTTATGTGGCGTGATGGAGTCTCACTGAATTTTTATTTTT
CGTAAGCAAACTTATCTTTTTACTTGTGCAAAGTGCCTTACGCTACGAGACCATTCAAGATCATCTTCGCAATGCAGCCCTTGGGATTCAAAAACATTCT
TATCTTTCCTACTCCCAGCCCCACTAAATTGAAACCTGCAAAATTTACAGCTGGTGTTGTATTGTAAAACTCTTCCATATGTATAAATCTGGTGTTTTTC
CCCCACCCCTCCAATATTTCCAAAAAAAAAAGCAAGAACATTTTGTAACTCCAGGTCCCCAGATATGTAAATATGATGAACTTTATTAAAAAAAAAAAAA
AA
5' EST: CX924669 [+]gaggaaggaagccattttggagcttcgagtaagagaaacacacggagagcaaacaaaaacataatagttatacttatagtgagtaataaacaaagagaaa
cgacggaaacaaacggacaacacgacgacgaggagtccgacgtgtccgagcccgggattgacttgaaggaacggactttattcttagttttgggcttttt
ttttaataaaggaaattgattttgtgcaccagagcaagtgatttcactgttgacagactttaaggacaagcacttgcttgtgttgcaataacaaagactc
atatattgagcaagactttatctcttcatttcgaagagtcaaataaacttattacagtctctgtactgactttaaaccttgtgaagctgaaaagacacac
cttcaaagggacaatttaattaaatacaaaacaaaatgagcaccgccagaaccgagaaccctgttttaatgggtatgtccagccagaatgggcagctaag
aggcccattgaaacccagtgcagggcctggaggtggggggacacagacacaacaaataaaccagctgaaaaatgccagcacaatcaacagtggcagtcag
cagcaagcacagagcatgagcagcgttataaaacctggtgatgactggaagaagacattaaaactccctccaaaagaccttagaatcaagacttcggatg
tcacctccacaaaagggaatgaatttgaagattactgtttanaaagagagctgctcatggggatatttgaaatgggatgggagaaaccatctcccataca
ggaggagagcattcccattgcattgtcccgtagggatatcttggctagagcaaaaaatggaactgggcag
3' EST: CX924668 [+]tttttttttttttttaataaagttcatcatatttacatatctggggacctggagttacaaaatgttcttgcttttttttttggaaatattggaggggtgg
gggaaaaacaccagatttatacatatggaagagttttacaatacaacaccagctgtaaattttgcaggtttcaatttagtggggctgggagtaggaaaga
taagaatgtttttgaatcccaagggctgcattgcgaagatgatcttgaatggtctcgtagcgtaaggcactttgcacaagtaaaaagataagtttgctta
cgaaaaataaaaattcagtgagactccatcacgccacataaggatgcagacaggggggataaaaaaaaaaattatatatatatgtatgtattagaactgt
tgctgccagaggatcctgaaatgtcttaagcagtaatactgggtcatctggaagcaaaatgtttagaagcagaaggctgtacctaatttttaggcccctc
caaacaaaatggacaacagggttagcttcccatccccatgagaaacatgtcaaaaaaaaaaaaaggggggggcattaagtgcaactaaaaggtgaaaacg
cagtgcaaacatttggggtttgtagggtactgtcctgagacaagggggagggagaagatgatgacaatgcaggtttgatatatgctggtcagtgttc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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