XB-CLONE-3247416
Clone Name: IMAGE:7695751
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Gene:
megs
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161117 [+]
gttagctagcaccattgcactgcttctcctgaccaaaactacctgacaaatttgactgaaggaaggtgaaaataggaatttggcataacagaactcaagc
cagcgctccagggatcaaattagaaggacatagcctccatcaacccctctgcctggcagttagacatgggtacagctgcagacatggtggctacttctac
cttaaccctacaacttatgctggaccatttacatgaccggatcgttctcaatatgcagcaaaccctagatgcagtaagagcagaggtgagtgaattgtgg
ggcataactgaaacaactgaacagcgcctagaggtaaccgaatcagccattaatgtcaatgagggggaaatcatatacgccactgaaaacttggctgtta
ctcgggaatacatgtaggacattgataatacccagaggagacacaacatccgcctccgtggtgttgccaattcaatgaccaacatatccagttttgcagc
tgacttatttaaaagcctgttacctgatgagactgttcaggtagattatgtattcaggctacagtacaaaactaaaaacccacatatgccccgggacatc
ttggtcaggttacccctggtatcacaaagggaagctctgctgaaggctgcaaggaaaatgaacttgactaaattcaaaggcaccaaagtgcaactgtatg
ctgatttatcaccttctaccctagaaaagagaaaggccatgcggccattagtgatctggctgcaagaaaatgctatgaaatatcgttggctatttccctt
tgcaattacatttgaatttaagggttggatctatcagtttccaacactggaagaagcccgcagggtgctactagtggaccatgggatcagcctatccact
gatactactgctataccaactaagaaacaagtggttgtccaaaccccacgtgccagtcctacatactccactccttccacagatgccagtctggaacagg
atctcccttctgctgcatcacaggatttttcaggagctccaagcagtaactctaataccacctctgctgatgttatgcctccttctgcagaaaatggact
ggaaaatgcaaacccttctggagcaaatgagccagagatgtcaggccttccacagataaactctactcccagctctgctgataatatacctctttatgaa
gagaatggcctggtaaagtcagacctttcagggatacactctattccaacccctgctgaatatatgcatccttctggagacaataacctggagaagtcag
gccttctacacataaacggtactcctagttctgctggttttatacctacatctggagagattggccaggagatgtcagactttccagagatacactatac
catcatctctgctgaatatatgcctctttctggatacaataacctagagaagtcaggccttctacagataaacagtcctcctagttctgctggttttata
cctacatctggagagaatggccaggagatttcaggctttccagagatacactataccaccatctctgctgaatttatgcctccttctggagacaataacc
tagagaagtcaggccttcaacagataaacagtactcctagttctgctggttttataactacatctggagagaatgaccaggagatttcaggctttccaga
gatacactataccaccatctctgctgaatttatgcctcgttctggagacaataacctacagaagtcaggccttctacagataaacagtactcctagttct
gctggttttatacctacttctggagagaataatctagacaagtcaagcttttcatatatacactatacccccacctctgctgaatatatgcctccttctg
gagaagataacctggagaagtcagagcttccagagataaatccgactcccacctctgctgattttctgcttcattctgcagagaataacctggagaaatc
agggcttccagagagaaatcatattcccctttctgttgattttctgcctcattatggagagaatgtcctggatgagtttagttctcctgagacaaactct
gtttctagccctactgataacatgtctacatttggagagaatggcctggagaaggcaagtgcttctgagacaaactcttcttccagccctactgattgta
tggcttctaatggagaaaatgacccagagatgtcaggcactccagagacaaaaggagacagtctgtcttgttgtgagggtgttgctgaagcatagcgaag
gaacatggaagaatgctccaagtggtggctgactccatactattctacagaaatagtacagaatatatgaattgtaacaattcataggacatacataaac
cttgggaaaagagcaatggaataacctacaatatcccttattgtcaggcaagagacaatcccagcaaaggaattaataatgacttgtgacctgttatcac
tgcactaatcccctggattatccctttctgccttcaattcaccagcttctcctgttcaacagtactttaagagaaaaaagtatatccgtggcatttgtcc
tctaggcacctctttatagactatggccattaacccagttgccaacaacttagttttctgtgttatttacatttaactctttaaagtcttaattctaatg
ctaattaattttacttcggcttccctactgtctgtatcaaattcattctacgaattcttaggtacaggatttatcatctgttctacatttctatacaact
ggttatgtttgagaaatatctacatttaagtagctataggttgtcttcttctagtcttaattttgaaaagttattaaaaattattaaaggttaatttaaa
ctaaaaaaaaaaaaaaa
5' EST: CX924798 [+]ttagctagcaccattgcactgcttctcctgaccaaaactacctgacaaatttgactgaaggaaggtgaaaataggaatttggcataacagaactcaagcc
agcgctccagggatcaaattagaaggacatagcctccatcaacccctctgcctggcagttagacatgggtacagctgcagacatggtggctacttctacc
ttaaccctacaacttatgctggaccatttacatgaccggatcgttctcaatatgcagcaaaccctagatgcagtaagagcagaggtgagtgaattgtggg
gcataactgaaacaactgaacagcgcctagaggtaaccgaatcagccattaatgtcaatgagggggaaatcatatacgccactgaaaacttggctgttac
tcgggaatacatgtaggacattgataatacccagaggagacacaacatccgcctccgtggtgttgccaattcaatgaccaacatatccagttttgcagct
gacttatttaaaagcctgttacctgatgagactgttcaggtagattatgtattcaggctacagtacaaaactaaaaacccacatatgccccgggacatct
tggtcaggttacccctggtatcacaaagggaagctctgctgaaggctgcaaggaaaatgaacttgactaaattcaaaggcaccaaagtgcaactgtatgc
tgatttatcaccttctaccctagaaaagagaaaggccatgcggccattagtgatctggctgctagaaaatgctatgaaatatcgttggctat
3' EST: CX924797 [+]aaattaacctttaataatttttaataacttttcaaaattaagactagaagaagacaacctatagctacttaaatgtagatatttctcaaacataaccagt
tgtatagaaatgtagaacagatgataaatcctgtacctaagaattcgtagaatgaatttgatacagacagtagggaagccgaagtaaaattaattagcat
tagaattaagactttaaagagttaaatgtaaataacacagaaaactaagttgttggcaactgggttaatggccatagtctataaagaggtgcctagagga
caaatgccacggatatacttttttctcttaaagtactgttgaacaggagaagctggtgaattgaaggcagaaagggataatccaggggattagtgcagtg
ataacaggtcacaagtcattattaattcctttgctgggattgtctcttgcctgacaataagggatattgtaggttattccattgctcttttcccaaggtt
tatgtatgtcctatgaattgttacaattcatatattctgtactatttctgtagaatagtatggagtcagccaccacttggagcattcttccatgttcctt
cgctatgcttcagcaacaccctcacaacaagacagactgtctccttttgtctctggagtgcctgacatctctgggtcattttctccattagaagccatac
aatcagtagggctgga
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: