XB-CLONE-3246529
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Clone Name: IMAGE:7794601
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Gene:
hydin
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161431 
[+]
gtgtccttggatagatcacatcataacttccattccatttttgttggtcatgaacttgaagagatagtatacctaataaacaatgaaaataagccattct
cctttgcttttcgagaaaactctagattttcggagggatttagccaaagtttgctggtaaaacctatggaaggaaccatcacaccattgtccaggttgcc
cgtctgtattttaatcaagccaacccaagtaggagaattaaactttaacctgatctgtgacattcgtacaaaaacggaaccactttgcctgaatgtaaag
gcagaaggacacaccactgatgtgtgtgttcagtgtcaagacggcacaggtgcagtaaccaccctgacttctcaggaaaccaatgaaattgaccttgggc
aggtggatattaatgacagcacgtcacttcagtttaatattatgaacaatggtcagtttccgttcaatttttcgtgtgcactgtctgctcctgagactgt
aaaggactgtataaccatccatccagacagtggctatgtgggacctgggcaggttgcacacttctcacttgccttttatcccaccaagaaatgtacactt
aaggacacccagctgattataaagatagaaaatggaccagagttaagatgttgtcttaatggcagagctgtcaggcctggaatgcatttctcttttacgc
agtataactttgggaattgctttatttaccatgctggaatgcagcctgttcggaagactctaatcattaccaacaaagatgagagggaggttagcatgga
ttgtctgtacagtaacacagcccatattgaggttgacttccacgcagttgtgctgcttccaggaggaaaaatggagatccctatcactttctacccacgt
gcagctatcctgtaccaagaaactgtgatctttcagatgaatgggcattccaagcaggcagtcaatcttcaaggccagggcattgagatgaaggttgaag
tttctgatcccaaatataaggtgacaaattttggagctgtaaacattgggcaagctgtgaagaaaagcatcccaattgtaaataatagcctttgcccagt
tacttgcaaaataacactaagtccaagtattgctgcactccaggatcccaaggtcctcagtatatcacctggttcagaaataacaatccccccacgtggc
ggcatctgtaaattggagctgcagtttaatccaagggaacgaattgtgcagtttgcagaagaagttatgcttgaagcttatggagtggcacgatcgcttc
ttgtcctgcgtgggtgcagccagggtctagaactgagcttggatcaggaatacatctcatttggagcagttgttttgcagtgccaggcaattagaaggat
agtccttagcaacaccggggagctgggagccaggtttaagtgggacagtgagaagtttcagccagacttttccatatcaccagcctctggttatataact
gctggcactgatgtgacatttgaagttgtcttccaccctcgtgagattgctgctgatattcactatgaaaatctttcatgcttcattgaagggagcaaag
ccttaatgttgaccctctctggatcttgtgtagggcttccctctaccaaagaggttgtgagtttccagtgccacgtgagaagtaaacagactcaaacgat
cttactaattaataaaactaaccaggcttggaatctgcatccagtcattgatggggaacagtggacagggcaggatatcatcgctgtggaagctcagcag
aacaagccctatgagatcacatatcgacctattaccatgaccagtgaggggaaaaaacaccagggttcaatcttcttccccttgccggatggggcggggt
tgatgtatcttcttcatggcattgctgatcctccaaggtcttctggtaatataatccgagaagtgccctgcaagacaccctacactgaactgctgtctgt
agccaactggctaaacaaaacacaaaggtttcatgcaatagtggaggcagttaagccagatcgtctggacagtactaccaccataaagggacttgaatat
attgaagttcctgctgcttcaaagagggactacaaactttcctttcattcttataaagagggcacattctctgtaaaggtgacattccgtaatgaggcaa
cccaagaatatctattctactttgtcactttcaaagcaacacctccaggcatcattaccactattgaaatggtgacaccagttcggcaaagcacagtagc
gactgtgaatgtggagaatcctctgtccgtcccagtcaccttcgtgactgattgtaaagtggcagagattaatttgccaccacaacttactgtacctgca
cagtctgagggtagtctgatgttcgagtaccaacctctgaaatctggggagtcaacaggacgtttagctcttcagagcactgacttgggtttatttcaat
atgaactccttctaaaagccacacctgccttgtctgagaaacccctttacttccgcacaactcttggctccagccagacaatcagtgccaaattcaccaa
ctatacccgccagaaggctgaatacacttgtaaggttgataattctgatttccacgttgagaagttagtgacagctgcccctggttcacaaggtggatcg
gaagtcagtgttgatgttacctatgaacctgtacagctaggggagtcacgtgctgttctgctgatttcttccccagtaggaggagagtacaccattccac
tttttggctcttccttatcccccaaacctcagggacccatccaaatccgctctggctcctctatcaccatccctttcaagaatgtctttctgcaaactac
agctttcacattccaggcagatcctccagctttcacagtgaagccctgtgagcctgttcgacctaaaaagacccattatatttctgtgtcatatgaggcc
cctaatcctgggagcaaggttccagtgactggaaagcttgtcatctcatgtcctcgtgccactggcattgcccaaggaatctactgggtgtattacctaa
agggtgtagtatctgagaagtgactttgccatactatcactatatgcactctctgctcagtcctcaaacacactcagttcacaattgaatgtttgttttc
ctataaagtacattacttgttgtatatgaataaaaaaaaaaaaaaa
5' EST: CX929231 [+]tgtccttggatagatcacatcataacttccattccatttttgttggtcatgaacttgaagagatagtatacctaataaacaatgaaaataagccattctc
ctttgcttttcgagaaaactctagattttcggagggatttagccaaagtttgctggtaaaacctatggaaggaaccatcacaccattgtccaggttgccc
gtctgtattttaatcaagccaacccaagtaggagaattaaactttaacctgatctgtgacattcgtacaaaaacggaaccactttgcctgaatgtaaagg
cagaaggacacaccactgatgtgtgtgttcagtgtcaagacggcacaggtgcagtaaccaccctgacttctcaggaaaccaatgaaattgaccttgggca
ggtggatattaatgacagcacgtcacttcagtttaatattatgaacaatggtcagtttccgttcaatttttcgtgtgcactgtctgctcctgagactgta
aaggactgtataaccatccatccagacagtggctatgtgggacctgggcaggttgcacacttctcacttgccttttatcccaccaagaaatgtacactta
aggacacccagctgattataaagatagaaaatggaccagagttaagatgttgtcttaatggcagagctgtcaggcctggaatgcatttctcttttacgca
gtataactttgggaattgctttatttaccatgctggaatgcagcctgttcggaagactctaatcattaccaacaaagatgagagggaggttagcatggat
tgtctgtacagtaacacagcccatattgagggtgacttccacgcagttgtgctgcttc
3' EST: CX929230 [+]catatacaacaagtaatgtactttataggaaaacaaacattcaattgtgaactgagtgtgtttgaggactgagcagagagtgcatatagtgatagtatgg
caaagtcacttctcagatactacaccctttaggtaatacacccagtagattccttgggcaatgccagtggcacgaggacatgagatgacaagctttccag
tcactggaaccttgctcccaggattaggggcctcatatgacacagaaatataatgggtctttttaggtcgaacaggctcacagggcttcactgtgaaagc
tggaggatctgcctggaatgtgaaagctgtagtttgcagaaagacattcttgaaagggatggtgatagaggagccagagcggatttggatgggtccctga
ggtttgggggataaggaagagccaaaaagtggaatggtgtactctcctcctactggggaagaaatcagcagaacagcacgtgactcccctagctgtacag
gttcataggtaacatcaacactgacttccgatccaccttgtgaaccaggggcagctgtcactaacttctcaacgtggaaatcagaattatcaaccttaca
agtgtattcagccttctggcgggtatagttggtgaatttggcactgattgtctggctggagccaagagttgtgcggaagtaaaggggtttctcagacaag
gcaggtgtggcttttagaaggagttcatattgaaataacccaagtcagtgctctgaagagctaaacgtcctgttgactccccagatttc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
