XB-CLONE-3246485
Clone Name: IMAGE:7794612
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Gene:
tmem165
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161030 [+]
ctgtccggcaggagcagaggagctggggaacagcggcactgcggggcgtttaagcagcgatccgggactgcggcctgtatttaagaagtttaggactgtc
ccacgaaaaaaaaacgaaaatgcatactgagattccagccgtcctctccagcaacactgagctctcctccacagcaaacctcggttttatccatgcattc
gtggctgcaatatctgtcattattgtgtcagagctcggggacaagaccttctttatagcagccataatggcaatgcggtataatcgcctcactgttctgg
ctggagccatgcttgccctgggactgatgacatgcttatcagttttgtttgggtatgcaaccaccgttattcccagagtctatacgtattatgtgtcaac
tgcgctgtttgcaatatttgggctccgaatgcttcgagagggattaaaaatgagtcctgatgaaggtcaggaagagctggaagaagttcaagcagaaatc
aagagaaaagatgaagaacttcaaagatcaaaactgctaaatgggactggggatgtggaaactggtgttgggccatctgtgcccaagaagaaatggatgc
agttcatctccccaatttttgtacaagcttttactctgacgttcttggccgagtggggagatcgctctcaactcacaaccatcgttcttgcagccagaga
ggatccttttggtgttgctgtggggggtacaattggccattgcttgtgcactggtttagcggttattggaggaagaatgatagcacagaaaatttctgtt
aggactgtgaccatcatcgggggcattgtgttcttggcgtttgctttctctgcgctatttattagtccagattctggattctaatgagatcccttgccaa
acatcacactgtgaatggatgctcccttttgtactgtactgctgtatataatgtacaaattgtagctagaatgatagccactgtacagaactgctcaatc
tcagagactatttttggagtctgtatgtaaccatcttccttcggtggaggattttttcttttctttggaccggcagattttagtgattcagagagcctcc
tccctgatagattatgtactattctcatgttgcaattggtcactagacaatctttagtaagctgtcagtattgggtagaatgagtaaggaagtttaacgt
ccatacttttttttttccccataatgtttttagttactttggtactgtgataaaataagaccttaaatcctttctgagtttgaaaacataaagcagaatt
ctctctgttgtatgtgcttatctttaaacatgactgcaatatggagataggcaacctgcacccccatgctgccttggtcagctgaggactgttggggaaa
tgctggagctgcttggtcagtaaacggttaaggatacttggttggacgttccagaactctgttttttttcttttttttttttagaaaaatgactggaaat
agttttttgtttcgcagcatcaattaatcataaaggattattttgtagcttctccctaaatactatatgggttacactattttatcatatgtgggtggtc
actggcagaatgtatcaacactaaacagatttacacctgggcattaacttgtgccacccaataacaatgcctgtattgttccacctgcagctggctggag
ataaagctaatcgctgattggttgctgtgtgttactgccataggtacagattagcccagagtattataggactatgtagaccaattcactgttattgtgc
ctttactaacattattatgttccaatcacatgcactgttccgtgaccgtaaacagtttcgttcccttagtattccagaaaagtattcagctttcttaatt
gtattcaggggaaaaggcttgttttattgcgttgtttctggctcgcttatcccaggcatttattgcttcatgaagtgcaatctcatggcttatagtagga
aaaaagtggcctgaaatgtatttgtataaaaagtttgtttttgctgtattgttccttgttttattttgcaccaatcttgcacttagggactgtcctttgt
gtttgcgctacttgaatgccttcggtgtggctcctaatgaacgagatcgcttcttttttctgaaaaacaaaaccgcacagcacatccttagaggggagca
gggagaggataagggcagcaccatgaaggactgcggttttccttctttataaagtctcttgatggtccattattgctctattccacagataatatacttt
atttggattacgtcatgtattgtgaggtgactgatgctcctagctagactgatcattagttcccagaattttctgaagtggttagttaccctttaatcag
tgaaactacatttataagcatgaagaaaaatgatttattagtatacataggctacctctgagctgggagttaaatgctgagagaagacacgataatgtgg
cagtaggtaaatagtaggtaattggttggcagaggtgacctgcctgggtgagcgtagcaacaaggacacatccaataacagcgcaagtagtcgtcatggg
gctactgacccctcccccagtcctttccacctcctatggctcatgtaaggggagcaggtgtttcacaatgtcacattggggtgactttactgattaaatc
ctgcccaggggggcgatagactttatctttagccccatcattatccttctgttcacagtgtttttaaaaacagattttttttcccttagtgtatggatgt
agctggaatagctttaatatcagtaaagaaatggttacaaagttagtgccaggtctagtaacctttaacaaccaatcagatgtcagaggtgaccagtaaa
tgctttctcctgattggctactaagggttactagacttaaaatgtttacattgcccccaaggtgttgcaataatagttatggaatgaataatgatttata
gtgcgcaattccaaaggctctggtaactgaaggaaggaaaatgaggaggaaatgctgtaaattattaaactatttttaagtgaaaaaaaaaaaaaaa
5' EST: CX929307 [+]ctgtccggcaggagcagaggagctggggaacagcggcactgcggggcgtttaagcagcgatccgggactgcggcctgtatttaagaagtttaggactgtc
ccacgaaaaaaaaacgaaaatgcatactgagattccagccgtcctctccagcaacactgagctctcctccacagcaaacctcggttttatccatgcattc
gtggctgcaatatctgtcattattgtgtcagagctcggggacaagaccttctttatagcagccataatggcaatgcggtataatcgcctcactgttctgg
ctggagccatgcttgccctgggactgatgacatgcttatcagttttgtttgggtatgcaaccaccgttattcccagagtctatacgtattatgtgtcaac
tgcgctgtttgcaatatttgggctccgaatgcttcgagagggattaaaaatgagtcctgatgaaggtcaggaagagctggaagaagttcaagcagaaatc
aagagaaaagatgaagaacttcaaagatcaaaactgctaaatgggactggggatgtggaaactggtgttgggccatctgtgcccaagaagaaatggatgc
agttcatctccccaatttttgtacaagcttttactctgacgttcttggccgagtggggagatcgctctcaactcacaaccatcgttcttgcagccagaga
ggatccttttggtgttgctgtggggggtacaattggccattgcttgtgcactgggttagcggttattggaggaagaatgatagcacagaaaatttctgtt
aggactgtgaccatcatc
3' EST: CX929306 [+]tttttttttttttttcacttaaaaatagtttaataatttacagcatttcctcctcattttccttccttcagttaccagagcctttggaattgcgcactat
aaatcattattcattccataactattattgcaacaccttgggggcaatgtaaacattttaagtctagtaacccttagtagccaatcaggagaaagcattt
actggtcacctctgacatctgattggttgttaaaggttactagacctggcactaactttgtaaccatttctttactgatattaaagctattccagctaca
tccatacactaagggaaaaaaaatctgtttttaaaaacactgtgaacagaaggataatgatggggctaaagataaagtctatcgcccccctgggcaggat
ttaatcagtaaagtcaccccaatgtgacattgtgaaacacctgctccccttacatgagccataggaggtggaaaggactgggggaggggtcagtagcccc
atgacgactacttgcgctgttattggatgtgtccttgttgctacgctcacccaggcaggtcacctctgccaaccaattacctactatttacctactgcca
cattatcgtgtcttctctcagcatttaactcccagctcagaggtagcctatgtatactaataaatcatttttcttcatgcttataaatgtagtttcactg
attaaagggtaactaaccacttcagaaaattctgggaactaatgatcagtctagctaggagcatcagtcacctcacaatacatgacgta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: