XB-CLONE-3245789
Clone Name: IMAGE:7698497
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Gene:
rnf220
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157775 [+]
GGCGCATTTTATATTAGACTCGCCTTTTTTTCTGGGATGACGCTTAAAGCGGGTCCGCGGTGGTTAATGTTGCAGGGTTAGAAATACTGACATCCACGGC
GTTTCTTAGTTACCATGCAAAGCGAAAGCAGGTCTACTGGCAGAGAGGAAGTAGACCTTTCTGAGAGTGAGAGCCAATAGGCAAACCAGACTTAATGCTC
GGATTGGAAAAATGAAACGAAGGAAGCAAGATGACGGGCAGGTATGTCCACTCTGCAGTCAACCGCTCTCTGGATCGGAGGAGGAGATGAGTAGACATGT
GGAACTGTGCCTTGCAAAGAGAGAAACTTGCATGAATGAGGATGATTCTGTAGATATTGAAAATGAGAATGGAAACCGATTTGAGGAATATGAATGGTGT
GGACAGAAAAGGATACGAGCCACAACACTTTTGGAAGGGGGGTTTCGTGGTTCTGGGTTTGTAATGTGTGGCAGCAAAGAAAATCATGATAGTGATGCTG
ATCTAGATGTTGATGGGGATGACACTCTGGAATACGGAAAGGCACAGTATACAGAAGCAGACATTATTCCATGCACAGGAGAGGAGCCTGGAGAAGCCAA
AGAAAGAGAAGCTCTTCGGGGAGCAGTTTTAAATGGCGGAACACCAAGCACCCGGATAACAACAGAATTCTCTAAATGGGCCAGTGATGAAATGCCATCA
ACAAGTAATGGAGAGAGCAGTAAACAAGAAACAATGCAGAAAACATGCAAAAACAGTGACATCGAAAAGATCACAGAAGAATCTGCAGTCACAACGTTTG
AAGCACTAAAAGCACGTATCCGTGAGTTAGAAAGACAGCTCTCCCGAGGGGACCGCTACAAATGCCTCATTTGCATGGATTCCTACACAATGCCCCTGAC
ATCTATTCAGTGCTGGCATGTTCACTGTGAAGAATGCTGGCTACGCACTCTGGGAGCTAAGAAGCTCTGCCCACAGTGTAATACCATCACATCACCTGGC
GACTTGAGACGTGTGTACTTGTGAAACAAATGTGAAAAGGGACAAACATTGCTCTGATGTCGTGGCAGCTCCCTCACATGTGATTCGTGGCCCTTCTCTG
TCCGTAGAACATCTCTGTTTGGGAAACAGACTGAGAGATTGGGGCTGCCTTGTGAGAAAGGAAGAAGCTAAAGATACCAACCCTTTGAAAGCTAGGAGCA
AAAGACCTTCCCTGGACTTGACTTCCCAGATAGGGAGAATTGAAGCAAGATTAAACTCATCCTGTGGCTTCCAGATGCTCAGAATGTCCTGTTAAATCCA
GATAGTACTAATGGGGGGAACTAAATCATTCCCTTACTGATATACACAGACACTTGTATACATTTTCTTTATCTTCTGATTATCAGAGTTAAATGAATGA
GTTTAAGGGGGAGCCAACATTCAATACCAGTCTGCTTTTGTTTACATGTCCTCTCCTTGCTCCATTCGTTTCACTACTTCAGCTGCCCAGGGTACACGTG
AGAAGCCGGACACTGAGGCCTAGGAGGCCAGGAAATAGAGTGACAGAGCGGACAATACCTAGCTGCGTTTTATTCTTTTTTTCCCTTCCTTGTTTCTTCA
TGTGCTGTTTTTGTGATTTTTCAACTGTGTAGAGAGGCAGGGAAAAGAAGAAAACTAAAAGAAATGCGAAGCACATGTATTATAAATTATATGTTTACAA
TGTTGTGTATAAATTGAATAGACTCAAAAAAAAAAAAAAAA
5' EST: CX930591 [+]gcgcattttatattagactcgcctttttttctgggatgacgcttaaagcgggtccgcggtggttaatgttgcagggttagaaatactgacatccacggcg
tttcttagttaccatgcaaagcgaaagcaggtctactggcagagaggaagtagacctttctgagagtgagagccaataggcaaaccagacttaatgctcg
gattggaaaaatgaaacgaaggaagcaagatgacgggcaggtatgtccactctgcagtcaaccgctctctggatcggaggaggagatgagtagacatgtg
gaactgtgccttgcaaagagagaaacttgcatgaatgaggatgattctgtagatattgaaaatgagaatggaaaccgatttgaggaatatgaatggtgtg
gacagaaaaggatacgagccacaacacttttggaaggggggtttcgtggttctgggtttgtaatgtgtggcagcaaagaaaatcatgatagtgatgctga
tctagatgttgatggggatgacactctggaatacggaaaggcacagtatacagaagcagacattattccatgcacaggagaggagcctggagaagccaaa
gaaagagaagctcttcggggagcagttttaaatggcggaacaccaagcacccggataacaacagaattctctaaatgggccagtgatgaaatgccatcaa
caagtaatggagagagcagtanacaaganacaatgcagaaaacatgcaaaaacagtgacatcgaaaagatcacagaagaatctgcagtcacaacgtttga
agcactaaaagcacgtatccgtgagttagaaagacagctctcccgaggggaccgctacaaatgcctcatttgcatggattccta
3' EST: CX930590 [+]ttttttttttttttttgagtctattcaatttatacacaacattgtaaacatataatttataatacatgtgcttcgcatttcttttagttttcttcttttc
cctgcctctctacacagttgaaaaatcacaaaaacagcacatgaagaaacaaggaagggaaaaaaagaataaaacgcagctaggtattgtccgctctgtc
actctatttcctggcctcctaggcctcagtgtccggcttctcacgtgtaccctgggcagctgaagtagtgaaacgaatggagcaaggagaggacatgtaa
acaaaagcagactggtattgaatgttggctcccccttaaactcattcatttaactctgataatcagaagataaagaaaatgtatacaagtgtctgtgtat
atcagtaagggaatgatttagttccccccattagtactatctggatttaacaggacattctgagcatctggaagccacaggatgagtttaatcttgcttc
aattctccctatctgggaagtcaagtccagggaaggtcttttgctcctagctttcaaagggttggtatctttagcttcttcctttctcacaaggcagccc
caatctctcagtctgtttcccaaacagagatgttctacggacagagaagggccacgaatcacatgtgagggagctgccacgacatcagagcaatgtttgt
cccttttcacatttgtttcacaagtacacacgtctcaagtcgccaggtgatgtgatggtattacactgtgggcagagcttcttagctcccagagtgcgta
gccagcattcttcacagtgaacatgccagcactgaatagatgtcaggngcattgtgtaggaatccatgcaaaatgagcatttgtagcggtcccctc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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