XB-CLONE-3243201
Clone Name: IMAGE:7706564
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Gene:
rnf20
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121446 [+]
GAACTAGCAGCTACCGAGCGGATCAGGGATTGCGGGCTGTATTAAAAAATTGGCCTGTTCTGCGGGAATACGTTTGGTGTCTGAAATAGATGTTGAAAAG
CTTACAGGGATGTCAGGGCCTGGAAGTAAACGTACTGTGGGGGATGTAGGTTCTTTAGGACCCCCTGAAAAAAAGGCAGCAGTGGAAGATTCTGGAACCA
CTGTCGAGACGATTAAACTAGGAAGTGTGTCTTCAACAGAGGAGCAAGATCTGAGGACCCTTCAACTGAAAAACAAGAAGTTGGCTGGAATGTTAGACCA
GAGACAGGCCATAGAAGATGAACTGCGTGATCGTATTGAGACTTTAGAAAGGCGGCAAGCAACAGATGATGCTTCTCTTCTCATCATAAACCGATACTGG
AGTCAGTTTGATGAAAATATTGGCATTTTTTTGGGTCGTTATGACTTGGATCAAGACTTGGGGGAGTTTCTGACAGAACGCAAAGCATTGGTTCTGCCAG
AGCCAGAGCCAGATTCTGACAGCAATCCAGAACGCAAAGAAAATGAAAGGGGCGAAGGACTATGGGAAGCTCCTCTTTCCTTTTTGGCCACTCTGGCCAG
CAGCAGCAGTGAAGAAATTGAATCTCAGCTACAAGAACGTGTAGAATCTTCACGACGTGCAGTCTCTCGTATTGTTTTGGTGTATGACCGACTGCATGAC
CAACTGGACCATCTTGCCAAGAAACTGAATAGCACTGATCCATCTCACGTGGAAGAGGCTGTCAGGGATTTAAACTCCTTGCTCTCAAATGAGAATGCAC
GATTGCAGGAGATTTGTAACTTGCTACAAGAGAAACAGCAGAATATGTCCCAAGAGTTCCTCCAGTTGCAGTCACGATTAGAGAGTGCTGAATCTCGAGT
CTTAGTTCTAGATGGTCACATTGAAGATCTTCAGTGGGACATTGATAAAATTCGGAAAAGGGAACAGCGCTTAAACAGACATTTATCTGAAGTTCTTGAG
AGGGTGAACTCGAAAGGTTATAAGGTTTATGGAGCAGGAAGCAGTCTCTATGGGGGAACAATCACTATAAATTCTCGCAAGTTTGAAGAAATGACTTCTG
AAGTTGATTTGAATAAAGAGCTGGCTGTAAATCGACTGCAAGAGCTTGAAAAACTGAGGCAGGATTTACAAGAAGTGACTTCAGAAAATCAGGATTTACA
GGCTGAGTTAGCCAGTGCAGTTGAAGAGAATGTGCGGTTGTCTCCAGAGTATCGCTGCATGCAGTCTCAATTCTCTGTATTGTACAATGAATCACTGCAG
CTAAAAACTCAGCTAGACGAGGCTCGTTCACTTCTACATGGAACACGGTCCAATCATCAGCGTCAGCTAGAGCTTATTGAGCGTGATGAAATTTCCCTCC
AGAAAAAGGTGCGCACTGAGGTTATTCAGTTAGAGGACACCCTTGCTCAGGTGCGCAAGGAATATGAAATGCTGAGGATAGAGTTTGAGCAGACCCTGGC
AGCCAATGAGCAAGCAGGGCCTATTAACCGAGAAATGAGACATTTAATCAGCAGTCTACAGAATCATAATCACCAACTTAAAGGAGAGGTATTGCGGTAT
AAAAGACGTCTGAGAGAGATCCAAGGGGATATAAGCAAAATGCGTTCTCGCAGTAGCAGTAGTCTTTTCCTTCTGCCATCACAGTCCAGCACTGAGGACA
CTCGTGAAGAGACAGCTGAAATTAAGACTGAACCTGAAGATACAACCACAAATGCTTCTGCCCTTTCTCAGCCAGAGGTAGTTCCAAAGAGGGAGGAGGA
GGAGGTACAGCCACCACTGCAACAACCACAGCGGGATAGAAGAGAGAGGGAAAGGGAGCGTGAAAGAGATCGTGGAAAAAAAAAAAAAAA
5' EST: CX958772 [+]actagcagctaccgagcggatcagggattgcgggctgtattaaaaaattggcctgttctgcgggaatacgtttggtgtctgaaatagatgttgaaaagct
tacagggatgtcagggcctggaagtaaacgtactgtgggggatgtaggttctttaggaccccctgaaaaaaaggcagcagtggaagattctggaaccact
gtcgagacgattaaactaggaagtgtgtcttcaacagaggagcaagatctgaggacccttcaactgaaaaacaagaagttggctggaatgttagaccaga
gacaggccatagaagatgaactgcgtgatcgtattgagactttagaaaggcggcaagcaacagatgatgcttctcttctcatcataaaccgatactggag
tcagtttgatgaaaatattggcatttttttgggtcgttatgacttggatcaagacttgggggagtttctgacagaacgcaaagcattggttctgccagag
ccagagccagattctgacagcaatccagaacgcaaagaaaatgaaaggggcgaaggactatgggaagctcctctttcctttttggccactctggccagca
gcagcagtgaagaaattgaatctcagctacaagaacgtgtagaatcttcacgacgtgcagtctctcgtattgttttggtgtatgaccgactgcatgacca
actggaccatcttgccaagaaactgaatagcactgatccatctcacgt
3' EST: CX958771 [+]tttttttttttttttccacgatctctttcacgctccctttccctctcgcttctatcccgctgtggttgttgcagtggtggctgtacctcctcctcctccc
tctttggaactacctctggctgagaaagggcagaagcatttgtggttgtatcttcaggttcagtcttaatttcagctgtctcttcacgagtgtcctcagt
gctggactgtgatggcagaaggaaaagactactgctactgcgagaacgcattttgcttatatccccttggatctctctcagacgtcttttataccgcaat
acctctcctttaagttggtgattatgattctgtagactgctgattaaatgtctcatttctcggttaataggccctgcttgctcattggctgccagggtct
gctcaaactctatcctcagcatttcatattccttgcgcacctgagcaagggtgtcctctaactgaataacctcagtgcgcacctttttctggagggaaat
ttcatcacgctcaataagctctagctgacgctgatgattggaccgtgttccatgtagaagtgaacgagcctcgtctagctgagtttttagctgcagtgat
tcattgtacaatacagagaattgagactgcatgcagcgatactctggagacaaccgcacattctcttcaactgcactggctaactcagcctgtaaatcct
gattttctgaagtcacttcttgtaaatcctgcctcagtttttcaagctcttgcagtcgatttacagccagctctttattcaaatcaacttcagaagtcat
ttc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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