XB-CLONE-3242430
Clone Name: IMAGE:7707568
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Gene:
gas6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121223 [+]
CTGAGCTGTACAGCTGCACGCAGCTGCACCACTTCTGCTGGATACACACGCATCACATGCATAGTTGCACTGCTTGGTGCTGCAGCAACAAGTCTTCAAA
AATAATCAGTCGAGACAGAACGCACTCTGGCGCAGCAGAACAGGAGCTACTGGAGGGATCTTGGCTCCACATCTCAGGCGCTACTAGAGCAAGGACGGAC
CTCTCTGTAGCTGGGTTTGTGCGCACAACATGAGGACGACATCTGCCCTGACAACCACGCTGCTTCTCACCTTCCTGGTTGCTGAGCCCTTGCATGGCAC
CATCGTACTGTCTGCCAAAGATGCTTCCCAGTTCCTGCGTCAGAGGCAGCGGAGGGCAAACCATATATTCGAAGAGACCAAACAGGGGCACTTGGAAAGG
GAGTGTGTGGAAGAGCAGTGCAGCAAAGAGGAAGCCAGAGAAGTCTTTGAAAATGACCCAGAAACCGAATATTTTTACCCAAAATATTTAGAGTGCAATC
GGCGATACAGGCTTCTTAACAGAAAAGACTCCCTGACAACATGTATACACAATATACCAGACCAGTGCTCATCAGCCCCATGTTATAGAGAAGGTTCCCT
CAACTGTATAGACCTGCACGGAGATTTTTTCTGCCACTGCAAGCCTGGATGGACTGGAAAAAGCTGCAGTACAGATATCAATGAATGTGCAACAGACAAT
GGAAATTGCAGTCAAATCTGTGTTAACAAGCCAGGAACATACCAGTGTTTGTGCCGTAGCGGGTACAGGCTTCATGTAAACAACAAATTATGTATAGATA
TTGATGAATGTGCAGAATCACGCAATATCTGTGGGACAGCCCAGTGCAAAAATGTGCGCAGCACCTACGTATGCCTATGTGAAGACGGATACAAATATGA
TGAGTTGACAAAGAGCTGCCAGGACATAGATGAATGCCTAGAGGGACGCTGTGAGCAGACTTGTGTGAACTCTCCTGGAAGCTACAGTTGTCACTGTGAT
GGGAGAGGAGGAGTTAAGCTATCCCAGAACATGAACACTTGTGAGGATATACTGCCATGCATTCCTTTTGCCTCCGAAAAAAGTAGAAATTCTCTATACC
TTGGTAGAATGTTCAGTGGCACGCCTGTCATCCGCCTGCGCTTCAAGAGAAAGCAACCCACCAGACTTATGGCAGAGTTCGAGTTTAGGACATTTGACCC
TGAAGGAGTCCTTTTCTTTGCTGGAGGCCATCAAGACAGTTCCTGGATTGTCTTGGCTTTGCGGAATGGAAGGTTGGAGCTGCAGCTGAAATATAATGGA
ATAGGCAGAGTAACCAGCAGTGGTCCACTTATAAACCATGGAATGTGGCAAGCAATTTCTGTTGAGGAATTGGAGCGGAATCTAGTTATCAAGGTCAACA
AAGATGCAGTCATGAAAATAGCAGTGTCTGGAGATCTGTTTAGTGTAGACAAAGGATTATACCAGCTGAATTTAACAGTTGGAGGCATTCCCTTTAAAAG
CAGTGAGCTCGTTCAGTCGATAAACCCACGCCTTGATGGCTGCATGCGCAACTGGAACTGGTTAAATGGTGAAGATACATCAATTCAAGAGACAGTCAGG
ATGACAGAAAAAATGCAGTGTTTCTTAGTTGCTGGGAGGGGCTCTTTTTTCCCTGGTAGAGGATTTGCCCTGTTTCACATAGATTACACATATCCTTCAA
AAGAAGAAAGTAAGGCAAACTGGAGGGTAGAGGTTAATGCACAAATAAATCCAGCTACTGATACTGGTGTCCTCTTTGCCTTGGTTTCAAAGGACATGGC
TGTCCCTTTATCTTTAGCTCTAATCGACTACCATTCCACTGCAAAAATGAAAAGACAATTCCTTATTATGTCTGTAGAAAACGTTGTTGTGTCCAGAATA
GAAATCCAGGTCTGTGACAGAGAACATATTGTGGAGATTTCTGTTAGCATCAATGATGTATTCCTTTCCTTTGATGGGACTCTCGGACAAAAAGAATTGG
ATGAATCACAGCTGCAAACTACTTTGCTTTTGCTGAATGACCACTTAGAAAAGGGTGTAAAGACATATGTCGGAGGATTGCCAGATGTTGCTGTCACTGC
GACTCCAGTCACCGCATTCTACCATGGCTGTATGACAATGAAGATTCAGAATAAACCACTAGACTTGGATGATTCTGTATACAAGCACAATGACATCACA
TCACACTCTTGCCCTCCGATTAAATCAGCCAAATAAGTTCATTGCCTTTTTCTCTTTACCAAATATTCAAAATGACTTTTTATATATAAATTATTCAGAC
TTGCAGTATTGTTTGTATAGTTTCTTAAAACACTGAAGTTAAGTAGGACCTGCTGATTTTTACACCGTGTTAAACTGTTTTTTCATGTTATCCCCTTAAG
TTGTGGTTCAAGTTTGTAATATATTTGTAAATAGTTTAAAAAAAAAAAAAAA
5' EST: CX960848 [+]ctgagctgtacagctgcacgcagctgcaccacttctgctggatacacacgcatcacatgcatagttgcactgcttggtgctgcagcaacaagtcttcaaa
aataatcagtcgagacagaacgcactctggcgcagcagaacaggagctactggagggatcttggctccacatctcaggcgctactagagcaaggacggac
ctctctgtagctgggtttgtgcgcacaacatgaggacgacatctgccctgacaaccacgctgcttctcaccttcctggttgctgagcccttgcatggcac
catcgtactgtctgccaaagatgcttcccagttcctgcgtcagaggcagcggagggcaaaccatatattcgaagagaccaaacaggggcacttggaaagg
gagtgtgtggaagagcagtgcagcaaagaggaagccagagaagtctttgaaaatgacccagaaaccgaatatttttacccaaaatatttagagtgcaatc
ggcgatacaggcttcttaacagaaaagactccctgacaacatgtatacacaatataccagaccagtgctcatcagccccatgttatagagaaggttccct
caactgtatagacctgcacggagattttttctgccactgcaagcctggatggactggaaaaagctgcagtacagatatcaatgaatgtgcaacagacaat
ggaaattgcagtcaaatctgtgttaacaagccaggaacataccagtgtttgtgccgtagcgngtacaggcttcatgtnaaacacaaattatgtatagata
ttgatgaatgt
3' EST: CX960847 [+]tttttttttttttttaaactatttacaaatatattacaaacttgaaccacaacttaaggggataacatgaaaaaacagtttaacacggtgtaaaaatcag
caggtcctacttaacttcagtgttttaagaaactatacaaacaatactgcaagtctgaataatttatatataaaaagtcattttgaatatttggtaaaga
gaaaaaggcaatgaacttatttggctgatttaatcggagggcaagagtgtgatgtgatgtcattgtgcttgtatacagaatcatccaagtctagtggttt
attctgaatcttcattgtcatacagccatggtagaatgcggtgactggagtcgcagtgacagcaacatctggcaatcctccgacatatgtctttacaccc
ttttctaagtggtcattcagcaaaagcaaagtagtttgcagctgtgattcatccaattctttttgtccgagagtcccatcaaaggaaaggaatacatcat
tgatgctaacagaaatctccacaatatgttctctgtcacagacctggatttctattctggacacaacaacgttttctacagacataataaggaattgtct
tttcatttttgcagtggaatggtagtcgattagagctaaagataaagggacagccatgtcctttgaaaccaaggcaaagaggacaccagtatcagtagct
ggatttatttgtgcattaacctctaccctccagtttgccttactttcttcttttgaaggatatgtgtaatctatgtgaaacagggcaaatcctctacca
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: