XB-CLONE-3241823
Clone Name: IMAGE:7708531
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Gene:
rec8
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122982 [+]
GGCGAAATCTAAATTGCATGTACTCAATAAATGGGAACGCGATCCGAGTGCAGCGCTGTGGGAAAAATAAAGAGATTAGGCAATTGAGCGAGAAAGGATA
TCAGTGGATAACTGGCATGAAAAAAGGAAAAAGTGACAACTTTCCAGGGGACACAAACATCTGAAGAGTTGGTGGAGAAAACGAATAGAAGAAAAATTAA
GAAGGGTGCAAAAATAGCAGCAGCATTTTGTTGACATGTTTTATTATCCAAACGTGTTGCAGCGCCACACAGGATGCTTTGCCACTATATGGTTGGCTGC
TACCAAAGGCACTAAAATTTTGAAACGAGAATACTTAAAAGTGAATGTCATCAACACGTGTCAACAGATTATGGAATACCTTCTCCTTCAAGTTCCACCT
CCACAGGTTGGCTTACCTGTACCTCGGTTTTCTCTGTACCTGTCAGCACAACTCAGTTATGGAGTGGTGCGCGTTTATCACCGCCAGTGTGATCTACTTA
TAGAGGAAATGAAAAATACTCTGGATCGCATTTACAAAGCTGAGAAACAGATGAGGATAGACATACTCCAGCCTGAACAACATGCCCTTCTCCCTGATGC
TCTGTCCCTTATGGAAATGCTGGAGGATGCGCCAGATCCTTTCTTTGGGATGATGGGAGTTCCTCCTCAGCTACCTGACCCATTGTTTATCCCACAGATT
CGCCAGATTCTGGAGGCCCCAAGCCCACTGAGACGAGAGCCAAAGGCAACACCAACAAAGCCTAAGAAAGCAAAGAATGGAGATGGGGGAGAGCATTTGA
CATCTCCAGAAAGAATAACTCTGCGAGAGGTGGAACCTATAACCTTTCCATCAATTGAGCTAATCAGAGACCTCCCAGAAGTCAGTGCTCATGATTTAGA
GTTCCTTATGTCTGATCTACCTTTGCTTGAAGAGGAACCCAGAAAGAAAAAGCCAAAAGAGGACATAAGTAAATTAGAGACATGGGAAGTTTTTCAGGTG
CCCACCAACTCCCTTTCAGAGTCACTGGAGGAGGCACGTAGGCGAGAAGGAGAGATACCAACAGCTGAGCTGTCTCCTACTTTTGGTCTCCCTTTAGAAC
CTGCCCCAGAAGCTGATGCTGCCACTATTCTGGATGAAATTACAGGAGAACCAGTTGTTGATTTCCCAGAACTGACTTCACCAGAAAGGTTGATTCCTGA
GGAGGTTTCACCTAGTGAGCGTTTTTCTCTTCCAGCCAGCCCTCAGCTGGTGCTTAAGGAGCTGCCTGTTTTGGAACGCTCAGTTAAGCGGGATGCTGTG
CGCAGAGGTGGGCTGATCATTGACAAAGAGACAAAGATTGCAGCTAAGAAAATACAGGAGCAGATACTAAGACCTGATATATACACTCAAGCAGCGGTTC
CTGTTGTGCTTCCTCCCAAGACTCCAGCCTCTTTGTTCCAGTCTCCAACCTACCAATATTGGATGGCGCCAGAACTTTCTGAACTGTGGACCCGCTGTGC
TCTGTTACAGCCCCTGACATATGCAGAGGAAAAGGAGAGAGAAGAGGAAACCATGAGCGAAGTGGAGGCTCTCCGTGCCATTGCTGAGCCTAGTGGTAGT
CTCATAATGTCCTCAGAAATCTCCCTGGAAGTGTCTGAAGAAGAGCGTTCTCGTCCTATTTTGCTAACCCCTGAGGAGAGAAGGGCCCTGTCGGTGCAGG
AGGAGAGATTTCTCCCTATGGTCTCAGAGATGCCAGAACTTATGGTTGAACTTCCAGAGACAGGAGACTTTTCCCTGGATGATGTACAAAGAAGGCTGCG
TACTCAGATTGACTACCTTGGCCAAACAGAGTTTCTCACTCATGCTCCTCACACACTCTCTCGCATTGCAGCCTCCCGACTCTTCTATAGCAGTCTCGTG
CTTTGCACACAGCGCATTATTTACCTGGAACAGAGGCTGCCATACGGCCAAATTCTCATCACCCCAGGGCCCCTCTACAGCCAGCACTGAGTCTCCCCCC
TCTCTCCATTCTGCAATGAATTCTCTCCTCCCTCTGGTATGCAGGAAACATAGGCACTGGGCTGCTAGGGTCGGTTTTAAATAAACAGAAGATTGGCATT
TTAAAAAAAAAAAAAAA
5' EST: CX961957 [+]gcgaaatctaaattgcatgtactcaataaatgggaacgcgatccgagtgcagcgctgtgggaaaaataaagagattaggcaattgagcgagaaaggatat
cagtggataactggcatgaaaaaaggaaaaagtgacaactttccaggggacacaaacatctgaagagttggtggagaaaacgaatagaagaaaaattaag
aagggtgcaaaaatagcagcagcattttgttgacatgttttattatccaaacgtgttgcagcgccacacaggatgctttgccactatatggttggctgct
accaaaggcactaaaattttgaaacgagaatacttaaaagtgaatgtcatcaacacgtgtcaacagattatggaataccttctccttcaagttccacctc
cacaggttggcttacctgtacctcggttttctctgtacctgtcagcacaactcagttatggagtggtgcgcgtttatcaccgccagtgtgatctacttat
agaggaaatgaaaaatactctggatcgcatttacaaagctgagaaacagatgaggatagacatactccagcctgaacaacatgcccttctccctgatgct
ctgtcccttatggaaatgctggaggatgcgccagatcctttctttgggatgatgggagttcctcctcagctacctgacccattgtttatcccacagattc
gccagattctggaggccccaagcccactgagacgagagcccaaggcaacaccaacaaagcctaagaaagcaaagaatggagatgggggagagcatttgac
atctccagaaagaataactctgcgagaggtggaacctataacctttccatcaattgagctaat
3' EST: CX961956 [+]tttttttttttttttaaaatgccaatcttctgtttatttaaaaccgaccctagcagcccagtgcctatgtttcctgcataccagagggaggagagaattc
attgcagaatggagagaggggggagactcagtgctggctgtagaggggccctggggtgatgagaatttggccgtatggcagcctctgttccaggtaaata
atgcgctgtgtgcaaagcacgagactgctatagaagagtcgggaggctgcaatgcgagagagtgtgtgaggagcatgagtgagaaactctgtttggccaa
ggtagtcaatctgagtacgcagccttctttgtacatcatccagggaaaagtctcctgtctctggaagttcaaccataagttctggcatctctgagaccat
agggagaaatctctcctcctgcaccgacagggcccttctctcctcaggggttagcaaaataggacgagaacgctcttcttcagacacttccagggagatt
tctgaggacattatgagactaccactaggctcagcaatggcacggagagcctccacttcgctcatggtttcctcttctctctccttttcctctgcatatg
tcaggggctgtaacagagcacagcgggtccacagttcagaaagttctggcgccatccaatattggtaggttggagactggaacaaagaggctggagtctt
gngaggaagcacaacaggaaccgctgcttgagtgtatatatcaggtcttagtatctgctcctgtattttnctagctgcaatctttgtctctttgtcaatg
atcagcccacctctgcgcacagcatcccgcttaactgagcgttc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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