XB-CLONE-3241623
Clone Name: IMAGE:7708340
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Gene:
ddx21
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157755 [+]
GGCGTGGTCAGCTGCGGACCCCGTTCTCTTGGATACGGGAGTTTCAGGACAAATCATCGATGCCCGTGAAGGTTTATGCCGATGAAATGGAGGTTGAAAG
TGTCAAGAAGAAGAAACAGTCTGAAACTCCTCTACCCAAGATAAAAAAGAGAAAAATGCAAAATGGGGCAACAGAAGATCTTGATTTGGAACAGGTGGCA
GAATCTGTAAATGGAGAGATAAACAACAATAATCCGACTCCCAAGCTGAAGAAAAAGAAGAAGCCTGTGCCACAATCAGATCTCTCTGAAACCGCAGAAC
AGTATGAAGGAGAGCAGCCAGATCCAAGCACTCCCATACCAAAGAAAGTCAAGAAGAAAAAACTAAAAGAGGGCAAGGAAGATTCAGACACACAGGAAGG
GACAGACAAATCCGAACCACAAATGAACGGCGTAAAGAGTGTTAAAAAATCTAAAAAGAACACAACAGGTGATGATAATGAACCAACTCCTAAGAAAAGA
AAAAAGGATACAATGGAGATCACAGCATCCGAGGAGTGTGAAGAAAAGCAGTTAACCAAAGAGGAAGAGGAAATAAATCAAGAAAAAATAGATGGCGACT
TCTCTAAATTTCCAATTTCCAAGGACACCATTAAGAATTTGCAAGCTAAAGGCGTGTCCTACCTGTTCCCAATTCAATCCAAAACATTCCACACTGCTTA
CAGTGGCAAAGATGTAGTGGTCCAGGCCCGTACAGGCACAGGGAAAACATTTTCCTTTGCTATTCCTTTAGTGGAGCGGCTTAATGAGGACCAACAGCCG
TTGGCTAGAGGACGTGCGCCTAGGGTGATAATTCTTACTCCCACCAGAGAGTTAGCTATTCAGATCACAAATGAAATCCGCAGCATCACTAAAAAGCTGA
AGGTCTGTTGTTTCTACGGAGGAACTCCATATCAACAGCAAGTTTTCTCCATTAAGGAGGGATTTGACTTTTTAGTTGGCACACCTGGCCGTGTGAGAGA
TCTTGTCCAAAACTACCGTTTGGACCTCACTGTTTTAAAGCATGTGGTACTAGACGAAGTTGACATGATGTTTGATATGGGCTTCTCGGAACAAGTCGAG
GAAATATTATCTGTTCGCTACAAACCTGATCCTGAAGAAAATCCCCAGACTCTTCTTTTCTCTGCAACATGTCCAGACTGGATGTACAATGTGGCTAAAA
AATATATGAAAAAACAGTTTGAAAAAATTGACTTGGTTGGACATAGAAGTCAGAAGGCTGCCATCACTGTTGAGCATTTGGCCATTGAGTGCACCAGGTC
CCAGAAGGCAGCGGTCCTTGGAGATATAGTTCAGGTCTACAGTGGTAGTCACGGAAAATCCATCATCTTTTGTGATTCAAAATTAGAAGCTCACGAATTA
GCCACAAACTGTGGCTCATTAAAACAGTCTGCCAAACCATTACATGGGGACCTCCAGCAAAAGGAAAGGGAAGTCATCTTGAAGGGTTTCAGGCAGGGAT
CATTTGAAGTTCTTGTTGCGACCAATGTAGCTGCTCGTGGATTGGATATCCCAGAAGTTGACCTTGTAGTATTGTACTCTGCACCAAAGGAAGCAGATGC
TTATGTTCATCGGTCAGGGCGCACAGGAAGAGCCGGACGCACAGGAATCTGCATATCCCTTTATGAACCAAGAGAAAGGCATTATCTTCGGAATGTGGAG
AGGAGCACGGGAATCACATTCAAACGTGTTGGAATACCTTCCTTATTGAATGTTGCCAAATCATCCAGTGCTGATGCAATAAAGTCCTTGGATTCAGTTC
CAGCTGATGTGATTGAGCATTTTAAAGAATACGCCCAGGAGCTGATTGAGAAGAAGGGTGCATTAACTGCCCTAGCTGCAGCTTTAGCTCATATTTCTGG
GGCGACGTCCATTAAACAACGCTCTTTGCTCAACATGGAAGCGGGTTATGTGACAATAACGTTGAAGAGCTCAGTGCAAATTCACAACTTAAGCTATGCA
TGGCGATCAATTAAAGAACAACTGGGTGAAGATATTGATTCTAAAATTCATAGAATGTGTCTCCTGAAGGATTCCATGGGTGTGTGTTTTGATGTCCGTT
CTGAAAACCTGCAGTCAATGCAGGAGGGCTGGAAAGATACAAGGCGGTGGCAGTTTACAGTTGCAACTGAATTACCCGCGATTCAAGAATCTGAGAGAAA
CTTTGATGGTCCAAGGAATGGAGGTTTTGGAGGAAGAGGGAGGAGACCTTTTGATAGAAGAAATAATTCCAGAGGAGGAAGAGGAGGAGGAGGAAGAGGC
AGAAATAGAAGTGGAGGGTTTAGAAGGGGCCGTTAGTGACTCCAAACATCCCAACATCCCATGTCACAATAACTTTTTTTAATATACTGCCATGTTCATA
AATATTCCTTCCTAAAGTAACGAGACATATTTTTATGCAATATAAAACTGTGTGCTTAGCCCTTATTAAAATCTATCTGTTAAATAAAAAAAAAAAAAAA
A
5' EST: CX962312 [+]gcgtggtcagctgcggaccccgttctcttggatacgggagtttcaggacaaatcatcgatgcccgtgaaggtttatgccgatgaaatggaggttgaaagt
gtcaagaagaagaaacagtctgaaactcctctacccaagataaaaaagagaaaaatgcaaaatggggcaacagaagatcttgatttggaacaggtggcag
aatctgtaaatggagagataaacaacaataatccgactcccaagctgaagaaaaagaagaagcctgtgccacaatcagatctctctgaaaccgcagaaca
gtatgaaggagagcagccagatccaagcactcccataccaaagaaagtcaagaagaaaaaactaaaagagggcaaggaagattcagacacacaggaaggg
acagacaaatccgaaccacaaatgaacggcgtaaagagtgttaaaaaatctaaaaagaacacaacaggtgatgataatgaaccaactcctaagaaaagaa
aaaaggatacaatggagatcacagcatccgaggagtgtgaagaaaagcagttaaccaaagaggaagaggaaataaatcaagaaaaaatagatggcgactt
ctctaaatttccaatttccaaggacaccattaagaatttgcaagctaaaggcgtgtcctacctgttcccaattcaatccaaaacattccacactgcttac
agtggcaaagatgtagtggtccaggcccgtacaggcacagggaaaacattttcctttgctattcctttagtggagcggcttaatgaggaccaacagccgt
tggctagaggacgtgcgcctagggtgataattcttact
3' EST: CX962311 [+]aacagatagattttaataagggctaagcacacagttttatattgcataaaaatatgtctcgttactttaggaaggaatatttatgaacatggcagtatat
taaaaaaagttattgtgacatgggatgttgggatgtttggagtcactaacggccccttctaaaccctccacttctatttctgcctcttcctcctcctcct
cttcctcctctggaattatttcttctatcaaaaggtctcctccctcttcctccaaaacctccattccttggaccatcaaagtttctctcagattcttgaa
tcgcgggtaattcagttgcaactgtaaactgccaccgccttgtatctttccagccctcctgcattgactgcaggttttcagaacggacatcaaaacacac
acccatggaatccttcaggagacacattctatgaattttagaatcaatatcttcacccagttgttctttaattgatcgccatgcatagcttaagttgtga
atttgcactgagctcttcaacgttattgtcacataacccgcttccatgttgagcaaagagcgttgtttaatggacgtcgccccagaaatatgagctaaag
ctgcagctagggcagttaatgcacccttcttctcaatcagctcctgggcgtattctttaaaatgctcaatcacatcagctggaactgaatccaaggactt
tattgcatcagcactggatgatttggcaacattcaataaggaaggtattccaacacgtttgaatgtgattcccgtgctcctctccacattccg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: