XB-CLONE-3241317
Clone Name: IMAGE:7708982
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Gene:
XB5854344
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161130 [+]
cgctgtccggggccgcgccagattgtctaacctcccgccttgttccgcccgcagcgactccttccgcctccgccaaacgaagccgcgtcccgagtagccc
gcagtgattatgcataaggagcacctggcccaggatgagaatagcaatccccgggagggccccggcagccggaagaaggacaagctgagccccagcgagc
aggacatgaaccacatcaacaagagcaaggcaaagacaggctcatgggagtctaacggctttgggccggacccagagatcgagacgttagcgggccgtac
ggaggacagcgtccctctcagcccttccaactccctcaacctgcgccacctgagaggctgcgagagagacccgtctgggcgccaacaccagcgctcccct
tcctcccatcaccactcctacagctactcgtcccaccatcaccactaccgccccttctactccggctaccgcaaaggctactgggactatgagagccaat
acagagatggcaagcgcaaagagtgggtgcccaggcccggcggtggcagtcgcacccaacgccccagaaatcgcgaagcctttaactccacagagtgcgt
ggatgggcggccagagcaggctggggtagagaaagcaaagactgtgccggccccagcgacaccccgccagccctcgcccccggctaaggaggagcacaca
gcagacagctgggtcctttttaaacccctgccggtgtttccggttgacagcagcagtgcccggacactgccaaaaatcagctacgccagcaaggtaaagg
aaaacctagatggcaaaggacttgtgcccagcagtgccctccgaacctccaacagcctccctaactgcctgctggccgccagtgtcgcagggggcaatac
cgaccccactagcccacagaccccaggctccctttgctcctcctcttgctcctccctctcctccgctcctccttcccctgtgagccaggacaacctgggc
gccatatttcagaatgagtggggactgtctttcatcaacgacccaggggctgggcaggcaccagttgcaagtcaggggtcgaaggacgggcccgaaggag
aggaggaaagtcacgctgctactccagtgtctgttttggatcgtctcaacctgcatggcttctacgtggacagagttgaggatgatgatgcctgcctgga
gaatcctagagactgggaggccatggtcaactacagtatgcaagagtggaatctagcttggaccatacacaagaaagatccttcccgtgtagtagtttat
gcagaatccatggatggaaagggctagtccctgcccctttccggggggcgcagagagccgcgagagccacgcccctcccccagacagccacgcacagagc
ccacagagcgagattcaggatgccacggaaacggtgccccacggaagcccctcccttccttacgctgcccggctctcctgtaggaggaagggctaaggag
gcatcttctcgacaaatagattttaacgctctccctgtttatgttctttttgtttttgcacctttgtatgacccctccaatgtgagaggtaacccctccg
aggcgcgagaggtttcatccacacactgggctttaattgcatcagactccataggagttggcttgttgctttccctgcagctgacgcctagccagacccc
tttctgttcttgcttttattctgtcggccgagccccctccctctgtcccacccatccatgcagtgtatattgactgggtgcacagcggtgtagattgcta
aggaatctgccccaacagctgccccaggctggatctgtacaagctccagtctccacgtttgttggcacggtttctgtacagggggcactgggcataaccg
gcgctgctccgctttgtgattggtcagggcctgatcccactaggaatcctgcatcccagtgcaatgcccccccttcccttcttttaaccctatccacgct
aaatttctggctgtgataccaaactggcaattggtttcctgccggcctgggatttatattgtgattaaagcaaaaaatctgttttaaaaaaaaaaaaaaa
5' EST: CX962810 [+]cgctgtccggggccgcgccagattgtctaacctcccgccttgttccgcccgcagcgactccttccgcctccgccaaacgaagccgcgtcccgagtagccc
gcagtgattatgcataaggagcacctggcccaggatgagaatagcaatccccgggagggccccggcagccggaagaaggacaagctgagccccagcgagc
aggacatgaaccacatcaacaagagcaaggcaaagacaggctcatgggagtctaacggctttgggccggacccagagatcgagacgttagcgggccgtac
ggaggacagcgtccctctcagcccttccaactccctcaacctgcgccacctgagaggctgcgagagagacccgtctgggcgccaacaccagcgctcccct
tcctcccatcaccactcctacagctactcgtcccaccatcaccactaccgccccttctactccggctaccgcaaaggctactgggactatgagagccaat
acagagatggcaagcgcaaagagtgggtgcccaggcccggcggtggcagtcgcacccaacgccccagaaatcgcgaagcctttaactccacagagtgcgt
ggatgggcggccagagcaggctggggtagagaaagcaaagactgtgccggccccagcgacaccccgccagccctcgcccccggctaaggaggagcacaca
gcagacagctgggtcctttttaacccctgccggtgtttccggttgacagcagcagtgcccggacactgccaaaaatcagctacgccagcaa
3' EST: CX962809 [+]tttttttttttttttaaaacagattttttgctttaatcacaatataaatcccaggccggcaggaaaccaattgccagtttggtatcacagccagaaattt
agcgtggatagggttaaaagaagggaagggggggcattgcactgggatgcaggattcctagtgggatcaggccctgaccaatcacaaagcggagcagcgc
cggttatgcccagtgccccctgtacagaaaccgtgccaacaaacgtggagactggagcttgtacagatccagcctggggcagctgttggggcagattcct
tagcaatctacaccgctgtgcacccagtcaatatacactgcatggatgggtgggacagagggagggggctcggccgacagaataaaagcaagaacagaaa
ggggtctggctaggcgtcagctgcagggaaagcaacaagccaactcctatggagtctgatgcaattaaagcccagtgtgtggatgaaacctctcgcgcct
cggaggggttacctctcacattggaggggtcatacaaaggtgcaaaaacaaaaagaacataaacagggagagcgttaaaatctatttgtcgagaagatgc
ctccttagcccttcctcctacaggagagccgggcagcgtaaggaagggaggggcttccgtggggcaccgtttccgtggcatcctgaatctcgctctgtgg
gctctgtgcgtggctgtctggnggaggggcgtggctctcgcggctctctgcgcc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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