XB-CLONE-3241261
Clone Name: IMAGE:7708852
|
Gene:
gcg.2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX962909 [+]
aactccaaactggagagcttcagaacaaaaactgaaataatttaaaaactacaaataatccaaaatgaagaccagttgcaaattgtcttagaatattatg
ctgtactaaaagttaactcaaaggtgaacaacccctttaaatcatttttaaatggtaccagaattaattgaatagtcccctaaatgtatgctgtcctaca
gtttaccttttatttaccccatgacaagtataaaaatgagattctactgcagataattgatgggtctgatgacaactcggatcttcagttgtcaactaag
attatccaggtaaatatgttatataataaatctaaaggtggccatacacgtggcgatctgacgatgtttcgtacgaccatcggtcacacggaacatcgta
agatccgccacacaccattcagggctgaatcggcaggtaaggaggtagaaacaataggatttctaccttctgccgattcagctctgaagggagaattttg
gtcggacgccttctattgcgcccgatcaaaattttcaaactggtccgattggcgagtcggccgatatcagcagcttcctgcgatatcggtcgactcgccg
acatgccatacacgcaccgattatcgtacgaaacgaggtttcgtacgataatatcggtgcgtgtatggccaccttaaccctgttaaaagagtgaataata
aataaaagggaagccttgtgtgccccagatactgcagaactacaacttccagttcctccatcagttggt
3' EST: CX962908 [+]tttttttttttttttagcattaacaataggaaatttttattttgcccaaacctctccccagtgctactgattcccgattacagtggtacagacggaatca
gagacaattacaatggaacaatcggaacaggcaaaggtgtacgaaacagtgaaatgtgccagagccgagcttctgagctccacacaaaaatagaggctga
attacatttaccaaatggaaagaaaaaactatagatttgtttcacttcacattcccagtagattctccattatgtgcgcctttggacccatttgcaaaaa
tagcttcattttactctatatagctctctgtaaatactttatcatctggatacaaatttattttcattggaactttcttctcgctcatagttacatgtca
aaagaaaataaacgtattaaaaataatgctattttgacttcaatttcgcaaggtgcctgaggcatgaacggactaaaacatcaatccacgcaattgaaat
atttgctttgtttttgaggctggagaccattggctcccatgattccttcatccgaccaactgatggaggaactggaagttgtagttctgcagtatctggg
gcacacaaggcttcccttttatttattattcactcttttaacagggttaaggtggccatacacgcaccgatattatcgtacgaaacctcgtttcgtacga
taatcggtgcgtgtatggca
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: