XB-CLONE-3240766
Clone Name: IMAGE:7709801
|
Gene:
f2rl2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX964603 [+]
tttttttttttcaagtttaaaaaaaataaacacatttttttattgaaaaattgaaatataagaggtaacaaatgctgctacacaacacagacaaaaattt
tgtgctattcatatacattaatataatagaggatccacgaataaactgtaacaaatgttgtaatgtcatcacataaattacacgtagtatttactttagc
ttggggttaaacgacctgtttaaagcaattgatacatttatacagtcatgtaactcctttgtgttatcaaatatccttatattacagtgggattgaatgg
ctgcccatgtgtactataggtactgatacactcagaggccttgtatgagctggcagcttccactacagaaaagggatgttctgtgttattccctaatata
tcacggtgatacaaggaaaaataaaactttatttatacatacgtatttaatgtgagtgtagttatactgggcaagacaggtttagtgcaaacatgcattc
ttgattatttacaatatagctaacattcacacagccattgtgaaactgtaacagccagcatcccttgtcagccccattgctagctggcataacattaaca
ggcaacataggatttatattatcagtattaaacacaattggctgcatgtatggtgggtgcaaaaatgatgcaaatctgcacgttagaattgcttacataa
atcaggtagaattaacatagctgtgcctcgcacaactgacgctttgtgcctattcattttgacttaggggcaaggcatatagcaagccctatagtcacat
agaaacccagggtgggcattaaatacagggttcccattgcatgctgcaccccacagcaattgtttattgagccacctgaatc
3' EST: CX964602 [+]ctattccttttgtgtggggttgctctggggctcaaagatgaggatacgctggagaaaaatgaatctgctgtacccaagacatttcggggaaagaaagaag
gtggggaatatgaagagctttcccatagcattctgcatggcctgccagacaccacgaccaataataatccttttctaagaatctccagcaataatgcctt
taaagtcgccagcgaacacaccatcactaaagtcagcaattcaaccttggtgtacctaagtggcaaagtaagcagggaactgataccgggcatctatatc
attgttgtgctcattggtgtgccagccaatgccttggtactatggatgctgttccatcaggtgacatctgtgtgcaccactgtgctctacgccagcttag
ccacctctgacttgctctattgcctcatgctgccttttaagatagcttatcacttaaacggcaacaactggatttttggagaaaccatgtgccgggccat
gaccattttcttgtactttaacatgtactgctccattctgctcctgatgtgctttagcatcaaccgctacattgccattgtccatccatttatatacagg
agcttgccgaaagggacctatacggtactcgtgtgcctacttgtgtgtgccattgtgcttgtctacatgatcccatttttcataaccaggcaaacctatc
acataaaggagctgaagaatatcattacctgccatgatgtatacgacatctcccataatgcttttcagttctattacttcatatcaatggtggtctttgg
atttctgatcccattttctgtc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: