XB-CLONE-3240717
Clone Name: IMAGE:7709960
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Gene:
acot12
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965740 [+]
catacaaaatcacagcttcattaataagaaaaaattcagtctttgtacaaatgcctcatttataataaagtgcaaaccgtgttcaagggtaaacattaat
cgttatcaaaattactgctacatagtaagtggcgatattctacaggagtctcctcaggatcagtagtgctgggagatcataacagtgttgtgtaaacctg
tgtcgtttctaggaatttaatgcaggaagaagccgtctcttcaatagaattggaccagccggtgagattctcttctacataaggcagaattcctggcgtc
agctgatttaagtaagaaacccggcagagatccttcccctggctgtgtatgagaaagcctgcacactgcacttcactcctcaggtattcagtggttggtg
gatgcgatgctagggtgaccgatctcatagctactacatatggatctccttgcttgcaggaataccttcgagacatcaggatcacaaagtccctggactt
actctgcttcacaggagggctggtaatgtgaaatattttatcatcctctccggctgtttctataacatcacagctcaaatagtgcatatcccagtttggc
cgcaatccaaggtcagacagcagaaaataagcttgttgcaactgaactttcacctgcatctccacttttatcgacaggatgtcattctcttcatgagtaa
atattctgatattgggcagggtgctgctcagctcccatccagtatttgcagccaggacagtcagcatggctatgttattgtagctgaggtaagcctggtt
gcctttatcccatgggtgagagacgggtctttcttgtttagttg
3' EST: CX965739 [+]ctatagggcgagaggccggctgcgctgccatattactatccctaaccagcgctaatactgcggttccacttactcccaggttacctgctgcctaattact
atcctcaaccagcgcaaatacttcgctgctccacttgccgctaagttaccggctgccccatttctatccccaaccggcgcttatactgcggctgcgctgc
cccattactataacctgccggcgctaatactgcggctgcgctgcacatagacatacatacaaactgaacagacgctactgccctgcactgcgattatagc
gccgcccctgcccttactcccatccctctcacatccccaggttgccctgcatgctgctgtctgggggagccccgaatgggctgggctgggttggggggcc
atggagagaatgctcagccgggtcagccctgagctctgggtacgagagaggagaggtgcagatgtgccacacagtgttcccctggcactccaaccaccgg
ggggagctcagtgctgggcagctcctcacatggattgataccactgcctgcctggcagctgaaaggcatgccggggtctcctgcgttacagcatctgtgg
atgatatccagattgaagagacagccagggttggacaaaatatctgcataaaagctaaagttaacagagctttcaacacaagcatggaggttggcattaa
agttgaaatagaagacccatttacaaatctacagaaacatgtctgtgcagccttttctacctatgtggcgaagccagctggttccaagaaggttttccta
aaaccagtgagacttcagtcttctcaggatcatctggaatacagcctggcagca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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