XB-CLONE-3240705
Clone Name: IMAGE:7710431
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Gene:
atp1a1
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tfg
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966036 [+]
ccaccattgtgctgcagggcaaggagcagcccttggacgaggaactgaaggatgctttccagaatgcctacctggagctggggggcctgggagagagagt
attaggtttctgccaccttgctttgtccgatgaccagttccctgatggatttcagtttgacacagaggaagtgaacttcccaacagagaatctttgcttc
gttgggctgatttccatgattgacccgccccgtgccgctgtgcctgatgccgtcggcaaatgccgaagtgctggaatcaaggttatcatggtgacaggtg
accacccaatcacagccaaggccattgctaagggagtgggaatcatctctgaaggcaatgaaacagtggaggatattgctgcccgcctcaacattccagt
caaccaggtgaaccccagagatgccaaggcctgtgtgatccacgggtcggacctgaaggacatggcacaagaacagatcgatgacatcctgaagcatcac
acagagatcgtctttgccagaacatcccctcagcagaagctcatcattgtggagggttgtcagcgacagggtgccattgtggcagtgacgggggatggtg
tcaacgactcccctgctctgaagaaggcagatattggtatcgctatgggcatcgctggttccgatgtgtccaagcaggcagccgacatgatcctgttgga
tgataactttgcttctatcgttactggagtagaggaaggtcgtctgatctttgataacctgaagaaatccatcgcctacaccctgaccagtaacatccca
gaaatcacacccttcctcatcttcatcgtcgccaacattcctctgcccttgggcaca
3' EST: CX966035 [+]ttgtacataatacctagtttaaactttaggacagatctaatttaacacatcagatcattaaaaacaagccaaataatgggggttgccatttgcattgtgt
catagttttgacaaggccggctagtgtcaaacacatttttgtatttgaatgtctactatagagagcaggtactatcaaaagcagaaaagaataatttggt
ctctacttgaattaaattacacaacatttagtgaaaagcaacagtgtgctgagaagcacactaagcttttcctctgatgtctgaaacatcagtacaattt
aagcataagattggaatctattgggatggatatagacctagcagccattgccttggcagtaatagtgagagtgacatttaccggtaaccaggtcctggct
gagcgtatccctgaccaaatggtaggcgattgcgcgcatatgggttagggccacttgggggtggggttacagtacttccaggggattgtgtaaatcctgc
cctaggctgatagctccctggttggcttggagccagtccaggttgggaaacaggaggcggattgtagtttgcaggctgagaggcttgtgtagtgtaagtc
tgtggagggtatcccccagcctggtactgctgagggggctgtgaagccatttgctgttgctgccctgggaaccctggagctggagtctgtgctgcttgct
gattgtaagctggatactgagctggttgaggagtagcttgctgattgtaaccagaatactgcatgccgtagggttgctgaggctgggctggagcttgggg
ctggccttgaggagctggataacccagcagctgctgtggctgtggatactgttgatatatctgtccctgctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: