XB-CLONE-3240701
Clone Name: IMAGE:7710251
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Gene:
zranb2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966113 [+]
tttgttttttttcctggattttacattttcatacagcttatgccaggaaacgcaaattcgggtttctactgaattgactttaacgccattaattaataga
aatatttgtaacttgccatataacagtaatttttttgttttacctgtgttttttgtatattgtcctgatgttccatacatttaaactgtcttcattttca
atcaataaagatccagctccagatcacacaggggatcttcatccccgagaaaaagatcttatgcaagctctcgatcatcttcatctccagagaggggcaa
gaagagaagtcgctctagatcctcatctggtgaccgcaaaaaaaagagatcaagatcacggtcaacagaaaggtttgggttactgtagaattagaaggga
aatttaattgtttagcaataaaacattgggaaattacttacctacttggtggttttttttttttttttttattatacattgtaacagcagactgcgaaag
gatcaaatgtccctttctgcccatgtactgctcagtggtatagtttccattatattaaattctgcccacccctctcagtaagcaaggcagtatggcaagc
atagttcatggtctctcaacactggaagtgactatatgatagtgtgctggcaaaatgtttgggtgggaaaacagttatgaagggccatattgtaaatgtg
ggataagggtttaatatttgtggtgaaagaattttacttctatagcctccaaagttttgcctagacaaatgttttgtgtgtt
3' EST: CX966112 [+]tttatttacacataactacagtaaatcctctaaactgtactatacgttatctaaacaaaaataatttgtttcagaacaaacgctcttatttgcttactgc
taaatgataaaaaattctcaccttgtcagaacagttttgtatactaagaagaacagactaacttctaacatctggttaagaagttttgcacatatttaaa
tagtagcgttgaaactgcaataggcacaattacgattacagtttgaaaaacagtacattcaggaaaaaagtacagaactaaagacatttcttattaagta
aactgatgaatagagagacttgaaagcaaagccaatttgaacccaaataattgtatctggtatttaaatagttagaattgaatttaactatggtactgtt
acattctcagtaaagaagtttactagttgcggcattttagatatgctaggattgcttccaatacctaatacttaccttgtaatactaatgaagaaagtga
acatttttctctaacacaagatttattttgtaatactttgcattttaagccataagaatagggtgacatgatcaaatacaacttcttccatttcagcagt
aatggaaaaagaacgcatttatttaaaatgggcagaattccaagaaactagatcagagagttattacttattaaaaaaaaaatatatatatattgcagca
caataggtgacaaacatcatccaagcattaatgataatattttaagtttctgtgtcttacggcttaaaatgctaattttaaattaggacctcactaatgc
tgtaactgggagag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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