XB-CLONE-3240684
Clone Name: IMAGE:7710452
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Gene:
dnajc11
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966511 [+]
aaaaattcttgggaagatatacacttttatatgaacccaccttatgagatcgcgggccctggccacaggttttcaatctaactggatgggcgggcttcaa
tcaggagaaaccacaaggggccatttaggaaaaagcagaaaactaacgtagaatatctgttaggttgtaagttctttgggataggaagctgcttatgttt
agcctttcaggattccaggcacttctgcttacctacccgctgatcagaaaggcttcccatccgtgctgctgtagctaagtgcttgagagatacaaacata
ttctgcatagatacaggatctacagactaaaataaaggaactcagggggacacaaacagattaaagtggatatttgtatgagggaaataaggaaaaacct
ggcgctagcacagcatggagagagaaatctcgatgggagctggatctgacggtagaaggagacaaggcagctataacagcagttggcatcaaaaataata
gggtgtatctatacttacacatatgtggaataagctccttcttgcacacgtgagtctattaatgcaatcattcacaaatgtctgacacaagtgtgcacag
ttctagactttgtgccctacctaccagtggcctgcaaacacgtgcctggcatgtccatgctcacagccgacagcaacacagagcatgtgcagagccactg
acaccaaagagagggccacgaatgacccgaagaatacagaaccatagtatgcttatatggagccatggatctttgctgttatacctttgggctgctgtag
taaaccttatgtataaccatttgtatcctgtatctggcctttacatt
3' EST: CX966510 [+]ttccatcaggcctgtagtatctaatactgccacatgggggcgcctgaagcaaatcattttacaagcaaaaatgatttgtatttattcgatcttatatttt
agtcctagtctttccttttttgttgcattttgtttgaaccaaggtaaagtaagaactcagggcacgtatttatgcacactatcatcatctgaacccacag
tttaattagggaattgtcaattatatatatatatataatgttgtgaaagtgcaacagtggggtgctgttggcattaatggcattgatcattatcctttat
aaaaagatgcattgccattagtctctgctccatatacaccctgtatttctgctgccatcatattttctataggggatacttgtctgtctcactggagggc
ctgaaccagacaaacctgcagcaggtttattttgtaacaccaatgccagagtgaaataaatcacatgtgaataaaacaggatagtttgaagtgatgtttt
taaacagactcttttccaacaaggaaggttacaactggagacttgtgtgccatggagctcactgagtgggttgccttatccagagcagccatttttccag
ttttatttgcatgctaaacccctccccctatagtgaattgtgtagtagcccatagttggcagtgagagcatactagaagcaggcgtctttctggaatcaa
ctccagccttgtgtctcacaggtaaagcacacgggtatgagccacacagagcacagccgtgattcacgacacatgttagtcacaaattactatccctgag
agtcattaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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