XB-CLONE-3240612
Clone Name: IMAGE:7711737
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Gene:
eef1g
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968175 [+]
tttcggtttgctgtcttgttaagttaaaaggctctggatgttggtacggatgtgaaaaccagactctgcgcgtattttagggggacagagatcacctcct
cctcatttgaagatcttgccctggttaaaggcctttcccacgtgcttgaagtctccctcccagcagaagtattctttgaccagggttttgcactcctcac
tattgctgtccagctttctccaggtgtaggattcatagtcaatttgccagtcttcagagagtgtgaatgcaaggtcctggcccctgaacacccacacgcc
ggagatggagctgtcattgttggtgccaaacaagatgacgctggcaaagccagtcttgcgcagtttgtctagacgctggaacatacctgtaattaagttg
cagctcatgaaggtttgtgtcagctcctcggggaatttgtattcagcgtaccagatggaccagccttccttgtcaaagtgctcccagaaataaggcagag
ctacagccagggtatcctcattagagtattttctcttaaactcatccattacaaaagagcttttaggtagatgtgcgtatgggtcctttgatttaggctc
tgctgctaaagccttttcagattcatccatatcatcctcaggggctggggcaggagcaggagctggtgttgccttcttcttttcctccttttcttttttt
ggctcctttgctggcttctctttcttgggagtttctttcttgggctgcatctcggcaaacttcttagcatcaaactgtgccatcttgtcacaaagcttga
cttctccccacacagcacggaattctggctgattcacacaggtcacaaaccaccttgtaacattaccc
3' EST: CX968174 [+]gccccatcaaggttgcctcctctcctccagaattccagtttggagtaacgaataagacatcagagttcctaaagaaattccccttaggcaaggtaccagc
atttgagggcaacgacggtttttgcctttttgagagcagcgccattgctcattatgtgggcaatgatgagctccgaggaaccaatcgtttacaccaagct
caggtcatccagtggctcagcttctcagacagtcacattgtccctccggccagcgcatgggtctttcccactttagggatcatgcaatacaataagcagg
ccacagaacaagccaaggaggagataaaggctgtgctgggtgttttagattctcatctgaagacacggacattcctagtgggcgagagggtcacgctggc
tgatatagcagttacttgctctcttctgtggctctataagcaggtcctggaaccatccttccgccagccatttggtaatgttacaaggtggtttgtgacc
tgtgtgaatcagccagaattccgtgctgtgttgggagaagtcaagctttgtgacaagatggcacagtttgatgctaagaagtttgccgagatgcagccca
agaaagaaactcccaagaaagagaagccagcaaaggagccaaaaaaagaaaaggaggaaaagaagaaggcaacaccagctcctgctcctgccccagcccc
tgaggatgatatggatgaatctganaaggctttagcagcagagcctaaatcaaaggacccatacgcacatctacctaaaagctcttttgtaatggatgag
tttaagagaaaatactctaatgaggataccctggctgtagctctgccttatttctggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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