XB-CLONE-3240590
Clone Name: IMAGE:7711576
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Gene:
slc3a2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968382 [+]
gtgaagtacaagctacattcattaggcaagtatatgtgtgggtggaaagagggtgggctatctatcatgttttgaaccgatttacaaggtgtttaaccat
catctgttatgaattctgtttaagtagataataatattgcttttttcccacagattggagcaccacaaatgggccatatggcttctttggtcaacgaaaa
gctatttcgtgtgtaccagctgctcctcttcacactgccaggcacaccaatctcattgtacggggatgaaattggacttaaagaccttcctagcaagcct
gctcagtcttcaagacctaacatgcagtgggatgaagtttcagtggctgctaattccccacaaatcttatccgatgtaaatgcaaatgtcacatttaagg
tgagaatagctctagagctaaattacaatatgatgctgtattgatgttacaatgaaaaacactactgttacattcaacatcgactgtaatcaagtaagta
gcccctattgctcttgtgactgcttggaaggttgttgcacaacaggactggattcttgctgccttgttgttgccatgtatggccaacttggcctaagtag
aaccacattgaggattttagccacttcttattaccctgtcctcaccaaggcaagctgcatatattctgtggtgattctgacaatgttggcaagaaaagta
gctgtagataatcactgctgatatacctggaaggctcagcaagaagtatatagccaatttagattattgctagtgaaacaaaatgaggactgcagagcta
gcaggggcatttacagtcactggtctgctgggtgtcccaatgaacacataggtgctcatttatttgcact
3' EST: CX968381 [+]gaaacaaacactaactttattgaagtaggaattttttgctgaccagcaccatgttgcacaactgtaaccaaactttcccctccccaaatacacaagatcc
acagacattcatattcccctggctgcggcccatctctccatgctcagagggctaaaagtgccacagcctttaaaacatgaaatgacaacataaaaacaca
cctcagagcacgtgtatgacaaaactaagcagaaaacaacactccacttattgctcttaacgcgtttcagaaactgtttttaagtttttatcctctgaac
atgaaaaatatgaccgcttggcaaatataaatggcaggttacagtatatgccactgccacaaagggtagggatgtgttaaaaggtttaaagggaaggtta
acaacatgtgcttcttggcactggaaagaagggtcagcaagtggaatgtgtaagtgtttgcccctcacagggatgtagggggttatccactataagggta
tttaaggagtaaagcctctccagctcctaactgcaagctttttagggaaacactttccccttcttttcgttgggagctggaactcaacactactgtgccg
tgttctggcagctcttcgcctctgtcctttttcaaggagagttctacctctccctcatagttaaagttcaaggctgtcacatatcgctcattctggtccc
agctccttaggaaagctatggccttgtcactgttatacagtaatacaaattccccatgcaggagggatcgttctttcccacgcaggtcactcaatttcct
gtatatgttta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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