XB-CLONE-3240402
Clone Name: IMAGE:7712739
|
Gene:
apob
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX970194 [+]
aggtgaggtgtcccttaaaaatcctgaacgcattcagtacaaaatcaactggagagaagaagcagctatggatgtacttaatggtttgaaagaaaggtta
ccaaagatttccaactccatccataattgtgtcaataaataccacaaggagcattttggaatggaaatcagtgaagcaggtctaaagatgaaggaaaatc
tccaggataaaattgcatcaacctacagatctactgccaatgagatcaacaacatagaatatcagttgcagtcaacagccagaggagccagtgacaaata
taatgaaatcgtcaacagtgcccaaaaatggtatcaagatccaaaggatatgtcaatgttcttgaatgatggtgaatataaagacaaagccattgaatta
gtacgcgcctaccaaaataagatgaaagacttgattgatgcagctattaaatttgtgaaaaacacaagattccagttgccaggacaaagcagaaagtata
caggtgaagagctatttaaccttggtatgaaaaatgtagcgagcaacattgatgcgttttttaaagccatgcaacaaatttatgatgcatgtgttcagta
tattaaccgcactgagcttaaaattcccggtactgaagtaacaatcaagggcaaagaaattacagatgcatgcaaaagctttagcgctaatctggaacga
atgaccaaaaaggccatacaggaccttcaaggcgtaagtctagaaagtgcactcaatcagcttaaaagttttatacaggtgatcactcagaaaacagtgg
atatgatcaagggctttgc
3' EST: CX970193 [+]aatgatgcaacctcattttaatgatgattttgcataatgcaaacaatgaaatgataatagttaaaaataatgccatcctataagaaacaatctttttatt
agcttacaataacgaaaacaaatttttgaattgttcacacaaaaacacacatttgagggacgtgtaaaccggttgttgctactgtacttgcaaacaacac
aaaagcctacgtttcagctatgccataatatacagcctgctcagtttttttgccagttccgaatgttctcaatgatctgctctgcagttagtttcccttc
ttgaagttgtctctcgataaactgctgcagctcttcccatttctgagaacctttgtcgatgccatccagtaccatggcgcgcgcaatttctgctcgtttg
cttataatgtcctctcttaaatgcgggatttcatcgaaggatttccagtcaaagggatgagggacttcaaccttaagttctcctttacgtgtggatatat
aggtcttcatttcttcagtggccgaattctgaatgctttggagaagctggttgacatactctatgaaagagttgtagttttggatgataatgtcaatgag
tcgacttgcctcggcaataagcttctcgtatgaatttacaaacaggttagatgtagcttgaagtttttcattaagaatcttattggcattttgtgaaagt
tgcctggcagttgcagaccacttagttactctatcttcaagatctattgaagccctccttaagagttctttgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: