XB-CLONE-3240344
Clone Name: IMAGE:7713093
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Gene:
slc13a2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971024 [+]
ctttttggtccctctctttcttctgcccctgcccctggtagtgcccaccaaggaggcaagctgcggctttgtgatcattgtgatggcgttattctggtgc
acagaagctcttcctctggccgtcactgcgctttttccagtcttgctttttccaatgatgggcatcatggattccacagcggtgtgcagccagtatctga
aagacacgaacatgctgttcattggcgggctgctggttgcgatatcggtggagaaatggaatttgcataaacgcattgccctaagagtgctgctgattgt
tggagttaaacctgccctcctgcttcttggattcatggtggtcacagccttcctttccatgtggatcagtaacacagccacaacggccatgatgatacct
attgcacaggctgtgctgcagcagcttcacacctcggaagagagagtggacaagccgtcagaagaaacaaaaaagaatgtgaatggaatggataatagca
tgtatgaatcagtattgccaaatgggaaaatgatcctggcagtagaaaacacttatgctactgtaaacgagggatttgagatgcaagatagtactaaaga
tcctgagccttccaagctagaaaaagaaagtattggcccaatagtgatagaacttgaggatgaaaaacaaaaggaagagaagcagaaacttagattggag
aagcacctgaagatctgcaaaggaatgacccttagtgtctgttattctgccagcattggtggaattgcaacattaactggcactaccccaaacttgtaat
gaaaggacaaatggacgagctctttcc
3' EST: CX971023 [+]ctgaaacctagggcacctagatatttcacccaatactgatagagccattaaaatgtttacattatttttcttaaataaatacaagtgagatacttgccct
gatacaaaaaattaagttcttagagagaactttttacactcttgcctgctgggtattgcccttattaacagtactattaactgataaggtccccaattaa
aagctttgcagagcagagtgagatgctaccttgactattctgtaggccggtagggtactcggcccatctgcttatagtcacagggtgggcacggcttgtg
cgtattcacggacactagagagtctgaactgtcaatctgtggattctggcagaagtcagtggggctgctgtaagatgccataaagaatcactgtttattg
ggctgatgggggactgtttgagtctcttggtagttgaaatgccagggtctattttgaattccagtctgaacctgtgccacatccaccactttatcatact
tctgaggctccttggcatccagttaaacacaactggccattaacagtccattacaaatgctaaggctgccctgtggagtttgcccaggatggaaatgtgc
ccaagtcgaacatgtaaaatccccagctgttaatggccagtgttatagtcagcacacccagaatgttgagcagtagtcctgcctttgccatgtcgataac
cttgagctgcccataagagaaggcaatggcattgggtggcgttgcaactggcagcataaaagccaaggaggccgatagtgtgcatggaagcatgatatac
agtgggttcagatggattgctttggccatggatgctaggatgggtaagaatagggttg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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