XB-CLONE-3240332
Clone Name: IMAGE:7713020
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Gene:
actr2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971184 [+]
gggaaattcctcttggaagaatcgcacttggcactgtcttacagcaacagcaaatttattcactgcgcttctatagaaacgagcgcacaaaggaaaacaa
taaaacaaaagaaattcatagatagataggaattctctagattttgttggaataatcacaaacacatttgtaactttttaactgttctgataatttcttg
tcaatgagcttgttcccagtggaatgggtcacctgatcaagtgcttcattaaaaggcaaaaagaaaaaaagtccagcaattcccatgttacatggtgctg
tttgcagggctggatgaggcccacagcaaagaaggggggataaaaccatttaaatcagggcacctctggctgctgaagaactacatccccacaaaaccct
ggcagcctttagcagttgggtaagttcaacagcagctggagggccactgagttactatatgtaagtgatgcctaacaatgattatcacacaatatgggca
aaggacctccttcacactaatgctgcgccctaacaacagacatgactgtgtagaagtgtaccttctaagaatgaaacatggaaacaaaaaaaattaaaac
aaaaatttagattttataactaaagtgacagaaagcagcagagggatttgtaaattgttgtacaaaataaatcacaattaccgataactgcttaaaaaaa
aaatctagatttttttttgtgtacgagaaggctaatgtggaaaaaaggaaaaaaaaaagggtccatccc
3' EST: CX971183 [+]ccggtgccggcggaggagggaaacaacataatggacagccaaggcaggaaggtagtggtgtgcgacaacggcaccngggtttgtaaaatgtggatatgcc
ggctcaaactttccagagcacatcttcccggctttggttgggcgacctgtgatccgatctactgccaaagtgggaaacattgagattaaggatctgatgg
tcggtgatgaagccagcgagttgcgttcaatgctggaagtgaactaccccatggagaatggtatagtgcggaattgggatgatatgaagcacctttggga
ctatacctttggaccagaaaaactgaacatagacaccagagactgtaaaatcctgcttacagagccccccatgaatccaactaaaaaccgtgaaaagatt
gttgaggtaatgtttgagacctaccagttctccggtgtgtatgtagcaatacaggccgtactcacattatacgcacaaggtctcttgactggtgttgtgg
ttgattctggagatggtgtcacacacatctgccctgtgtacgaaggcttctctctgcctcacctcaccaggagactagatatcgcaggcagggatatcac
ccgctaccttataaagttgctcttactacgagggtatgctttcaaccactctgctgattttgagactgttcgtatgatcaaggagaagctttgctatgtg
ggatacaacattgaacaggagcagaaacttgcgctagagacaacagtccttgtggagtcttacacacttccagatggcagggttattaagttggtggaga
gcgttttgaggca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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