XB-CLONE-3240303
Clone Name: IMAGE:7713604
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Gene:
adss2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971965 [+]
cttgggttcttcgtgactttaacattctatatagtcttcagttagtatggcgtaacaaagcaatcagtcacgaggtggcgctaagtatatttataaatac
atagaatgagcgctaaagaaaagtcctggagagggtaggaggagtaggaagggaaagtggcattttgtatagaagcgtgtcaggtttgagagttctacag
gaagtagtgttataggttgctctgtacttctcccagcctttcggccatatcctttgtaagcttaacccactgtcagtaatcatgtcggcagaaaacggat
cgcctgggctgccaaacggcggagtatgctgcgccactgacagtggacgttgctccctcgtcgggaacaaagtcacagttgtgcttggggctcagtgggg
agacgaagggaagggtaaagttgtggatcttctggctcaggatgcggatatcgtgtgccgctgccagggaggaaacaatgcaggccacacggttgttgtg
gattcagttgaatatgattttcaccttttgccaagtggaatcattaatcaaaatgctattgctttcataggaaatggtgtagtaattcatctacctggct
tatttgaagaagcagagaaaaatttaaagaaagggcaaggtttggttggctgggagaagagattgtgtatctctgatagagctcacattgtgtttgactt
ccatcaggctgcagatggcatccaggagcaacaacggcaagaacaagctgggaaaaatttgggcacaacaaagaagggtattggaccagtctattcttca
aaagctgctaggagtgggctcagaatgtgtgatcttgtttctgacttcagtgaattctct
3' EST: CX971964 [+]cttgggttggaaccttaaactcttattcaataatttacaaatatagactgtctacccatttgttttgcacagtgcaagtattgcagtgcaatacatttta
aaacaaaatcattcacaccaattatgattgctttaaaaatatggatatttcctccccatacattacatgagttatgttaagaaatcaatagaaagatgta
aaaatgtgtaaaagaattctgattcagtctcacaacttgaaattgcagactgtcaacaaagttgtaccagatgcgcacatataggtactagggtgttttt
tttaattttatacagaaaaaatagccagttcaatgtgtgatggtcctttccaaaactgaacaaagaagtcactcagtctttaaagtactgccagtttaat
aaccagaccacaaatgtcctaatgcgatagaaaataaacagtaggacaattttgactgctttcttttactagattgtgaaaaagactaaataaattaggg
cataacaaaagatgaatctggaagtgatattttgttgcccatgcatttgttctctatgtacaagtgagaagctttatctgtagaatggtgtcatcttgag
gaggcagcctgtgcagtgtgcatcttcttagaacagctgaatcatcgactctctggattttccaacaccaatccatttaataggaattccaagttctaat
tctatatatcgaacatatttctttgcattttcaggcagctcttcnaatgttcgaacgttacatgtatctgtattccacccaggaagtgtctcatattcaa
cctctactctngtaagaactttctgattngct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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