XB-CLONE-3240281
Clone Name: IMAGE:7713947
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Gene:
slc7a7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972377
[+]
gagattatggacatctcttctcatcattgaacccacaagccaagctgtcattgccatcacattttccaactatatgttgcagcctatcttcccttcttgt
ggatctccttatgctgctaatagactcattgcagcaggttgtatagcactccttacctttgtgaattgtgcctatgtaaaatgggggaccagggtccagg
atatcttcacttatgccaaagtgctagctctgattgctgtcattatagctggaattgtaaagatctgtcaaggtcattcagagaactttgaaaattcgtt
tgctggctcctcatacgatgttggtgacattgctcttgcattatattctgcacttttttcatactcaggatgggacacacttaactttgtaacagaggaa
ataaaaaacccagagaggaacctgccattggccattgggatttctatgccaatagtgacagtgatttacatccttaccaatgtggcctattacaccgtgc
tggatatcagggaaattcttgccagtgatgctgttgctgtgacctttgctgatcagatttttggcatatttaactggacgataccactggctgtcgcttt
gtcatgctttggtggcctcaatgcatctatccttgctgcatccaggctgttctttgtgggatctcgggagggccatcttcctgatgcactcagcatgatt
catgtagaaagatttacacccatacctgctcttctctttaatggtgcaatggccctggtgtacctctgtgttgaagatattttccagctcattaattact
acagtttcagttactggttatttgtgggactttctattgcgggacagctgtatctgcgctggaaacaccagaccgac
3' EST: CX972376
[+]ttttttgtgcttcatttgtttttaatagatattgcacttgattgtatgtttttttctttctttcctgaataactgttatcaacacaactgctttatttta
gatggattaactgtttcatccaggagcacaaaaacttcaaaaggttttttccttccactggcatagccaaattccacaataaataactttgatttgaagg
gaaataagaaatcaatagtgtagatggctcccctttcgtgttggattagccaggctcatattgctttacaacacaaggctggcccttagatcagaaagta
gttaattccttgctttggaaataataaaaggtttaatatttatgctattcaactattccctcccagagagaaatggtttgcccatgtccatccagagaac
tgctctgcagcaaccttgcaatagaaaggtagtttttttttcttttttaaaaaaggcacagtttatgcaataaattagtaaggcttcaggtttctttgag
tcagccatggaatggtggacagttctatcaatcactttttgagaagtctttttcctcttctgtatccatttcagttagtactgagtagcacagaatttgt
gtatatcgtgtcagagcacctgcagcacgctgaagacattgtggcttccgtttctcagaaacacgaaccagaaggaaatagactgggactcctgacaatg
caattcccactccaatcagagaatttattagatcactatacaggggtacagccaccaggaacacagtgcanagacagaatatgattgggaacactaggct
gagcttgagtggccttggtcggtctggttgtttccagcgcagatacagctgtccccgcatagaaag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: