XB-CLONE-3240173
Clone Name: IMAGE:7714236
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Gene:
cpb1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973404 [+]
aaaaagtgttccgtgtaattccacaaaatgctgaacatgttgaacttatcaaatctatggcccaaacagaagggcttgatttctggctccctgactcagc
acaacttgtagaacaaggaaaacgagctgattttcatgctgatggtcatgtctcatatgaggttcaggcccttctccagcaaagtgggatgccctatgaa
atattgattaatgatctgcaagatgccctagaaaagcagcgcgacagcaacatcagggctgttcacagttatgagaaatacaatgatcttgatacgatta
atgcctggtctgccaacattgctgctcagaacccaggacttgtgtctcgttcctcgataggaacctcatatcaaggccgtcctatttacttgctcaaggt
tggaaaaagcggtgcaaacaagaaagctgtattcatagactgtgggtttcatgctagggaatggatctcccctgctttctgccagtggtttgttaaagag
gctgtgagtgcatatggcgttgaatctgagtttacctctctgctcgacaacctggacatctatgttttgccagtattgaatgttgatggttacgtctaca
cctggaccactaaccgtatgtggaggaagactcgttcagctaaccccaacagcacttgcattggaactgacccaaacagaaactttaatgctggctggtg
cactgctggagcctcaacaagagcctgtgatgaaacatactgtggctctgctcctgaatctgaaccagagacaaaggctttggctaacttcatcccgcgc
aacattcccctgcaatcagggtaccttactatc
3' EST: CX973403 [+]aatgtatttgtgattcatttatttgatttcattatacaccaactggtgtttctaaaataattaagaaaactttcagacctaagattaaaaatattaaata
caaatggacatcacaagacctttagcactaagttagctttgaataacttcatttagcattgttctttatgtagctggcaatgtatttgacggccagcatg
gtttcttcacaggtaggtttaatctgagattcagggagggcaaaaccatatcgtcctgtgtcacggagctcaaatgtgtatgagaatttaacgcctgcgt
cataggcccagtcatctgatccacctgctgcaaggtagattgttgatcctccgggaccgtaagtgtacttggttttgtacagtgaagtcagactgttaac
tgctccctgagcaactgcattcagttcattgtgatctttagcaactgcataactgtaggaataggggaacagcaacatctggctataagaatggatagta
aggtaacccttgattgcaggaatgtttgcgcggatgaagttagccaaagcctttgtctctggttcagattcaggagcagagccacagtatgtttcatcac
aggctcttgttgaggctccagcagtgcaccagccagcattaaagtttctgtttgggtcagttccaatgcaagtgctgttggggttagctgaacgagtctt
cctccacatacggttagtggtccaggtgtagacgtaaccatcaacattcaatactggcaaaacatagatgtccaggttgtcgagcagagaggtaaactca
gaatcaacgccatatgcactcacagcctctttaacaaaccactggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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