XB-CLONE-3240070
Clone Name: IMAGE:7715693
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Gene:
sorbs1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975625 [+]
ataaactaattactcgagcaatcaaaattgatgtgattttttttttacatggcaggaaaaatgatatggtacaatatatgacattgtaatcgtctatagg
aaattctgtgtactatagataacattcatgcacaaaaccatggggaaaaaactattttcaggcattgttcataatggtgctctaaatgctcaaatgcgcc
atcagtgttaatgggagtccgttgcctttattgctatctgaacagttataagggtgaagacacacagagctactggaggcagctacttgtcgaggttact
aaaatagacaatgctgatcatgtactaataattgtctctacgtgtgttttagcagaggcaattctcattattgtctatggcagggtattttctggcattt
agaagccatgacaagtggctgttactaagtggctctttgtgtcttcactctaaaataaagattattataaggtgattgctataagaagtagttgctgtat
ttgcaaattagaggggtggtggtacccattctgttttgagacaaaaggccattgattgcaaatatctccataatagtgacaatgcataattctggttggc
cccggacactcctttgctagaagcactgcttttgtgccttgagtgcacgtaggggcccttattctatgattatcacttagttggtgcctctcttataatc
tttgcatatcatctaaattgcctaagggacagatacggtaatataaactgaaatagaaggttttccctttngtgctctttcccttagcatgagagctcct
acagt
3' EST: CX975624 [+]gagaggtttggcataacaggatcattctaatcatttctgatttgaaacacggcctttttctattatgtcttgagtttctcagtttatcacacatatatat
atctattacattttataaggtggcctttttcaggtggtctcctctgactggacacccagctagtgatcttattggttatatgctgggcttgtgcctcatc
cagagggtgaagtgttctgataacaaacttacatccatagagttcaagctcttactgtaggagctctcatgctaaaggaaagagcaacaagctcttactg
taggagctctcatgctaaaggaaagagcaacaaagggaaaaccttctatttcagtttatattaccgtatctgtcccttaggcaatttagatgatatgcaa
agattataagagaggcaccaactaagtgataatcatagaataagggcccctacgtgcactcaaggcacaaaagcagtgcttctagcaaaggagtgtccgg
ggccaaccagaattatgcattgtcactattatggagatatttgcaatcaatggccttttgtctcaaaacagaatgggtaccaccacccctctaatttgca
aatacagcaactacttcttatagcaatcaccttataataatctttattttagagtgaagacacaaagagccacttagtaacagccacttgtcatggcttc
taaatgccagaaaataccctgccatagacaataatgagaattgcctctgctaanacacacgtagagacaattattagtacatgatcagcattgtctattt
tagtaacctcgacaagtagctgcctccagtagctctgtgtgtcttcacccttataactgntcagatagcaataaagggcaacgactcccattaacactga
tggcgcatttgagcatttagag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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